Histones and

Histone Modifications
Histone Lysine Methylation Pathway

Transcriptionally Inactive Transcriptionally Active

Chromatin Chromatin

NU PTF
Me

RF
NU PTF

B Me
Me

RF
B Me
Me Me

Me
Me
n
Pericentric Heterochromatin tio
M

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4m latio
H

h
hy

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4K hyla

la
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M K y
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H
M 1 9h V

M 1 9h V

M 1 9h V

M 1 9h V

n
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HP SU

HP SU

3 U

HP SU

Transcription Initiation
S

em
3

D
HP
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Methylation Methylation Me
Me
Me
Me
H3K9me H3K36me
Demethylation Demethylation

Pericentric Heterochromatin
Inactive X Chromosome
Rb-Mediated Repression M
et
H hy
hy me n

D 3K lat
7 tio

Transcription Elongation
3K hyla

em 7 io
et 9m n
n
t

tio
PC C1

PC C1

PC C1

PC C1

hy e
2
M
PR

PR

PR

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M

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Me Me

Me Me

Inactive X Chromosome
Hox Gene Repression Anti-Silencing

Methylation Degree: + Mono † Di ‡ Tri

H4K20me H3K9me ESET/KMT1E (†,‡) JMJD2B/KDM4B (†,‡) PHF8/KDM7B (+,†) NSD3/KMT3F (+,†) JARID1B/KDM5B (†,‡) JMJD1B/KDM2B (+,†)
Methylases: Methylases:
SET8/KMT5A (+) CLLD8/KMT1F (†,‡) JMJD2C/KDM4C (†,‡) UTX/KDM6A (†,‡) NSD2/KMT3G (+,†) JARID1C/KDM5C (†,‡) JMJD2A/KDM4A (†,‡)
PRDM3/KMT8E (+)
NSD1/KMT3B (+,†) Demethylases: JMJD2D/KDM4D (†,‡) JMJD3/KDM6B (†,‡) SET7/KMT7 (†) JARID1D/KDM5D (†,‡) JMJD2B/KDM4B (†,‡)
PPRDM16/KMT8F (+) AOF1/KDM1B (+,†)
ASH1L/KMT2H (+,†,‡) KDM4E/KDM4DL (†,‡) H3K4me SMYD3/KMT3E (†,‡) H3K36me JMJD2C/KDM4C (†,‡)
G9a/EHMT2/KMT1C (+,†) JMJD1A/KDM3A (+,†) Methylases: Methylases:
SUV420H1/KMT5B (†,‡) H3K27me ASH1L/KMT2H (+,†,‡) JMJD2D/KDM4D (†,‡)
EHMT1/KMT1D (+,†) JMJD1B/KDM3B (+,†) Methylases: MLL1/KMT2A (+,†,‡) NSD1/KMT3B (+,†)
SUV420H2/KMT5C (†,‡) PRDM9/KMT8B (‡) KDM4DL/KDM4E (†,‡)
ASH1L/KMT2H (+,†,‡) JMJD1C/KDM3C (+,†) Ezh2/KMT6 (+,†,‡) MLL2/KMT2B (+,†,‡) SMYD2/KMT3C (+,†)
NSD2/KMT3G (+,†) Demethylases: H3K79me
PRDM2/KMT8A (+,†,‡) JHDM1D/KDM7A (+,†) NSD2/KMT3G (+,†,‡) MLL3/KMT2C (+) LSD1/KDM1A (+,†) NSD2/KMT3G (+,†) Methylases:
Demethylases: PRDM8/KMT8D (†) NSD3/KMT3F (†,‡) MLL4/KMT2D (+) SET2/KMT3A (‡) DOTIL/KMT4 (+,†,‡)
JHDM1D/KDM7A (+,†) PHF8/KDM7B (+,†) AOF1/KDM1B (+,†)
SUV39H1/KMT1A (†,‡) JMJD2A/KDM4B (†,‡) Demethylases: SET1A/KMT2F (+,†,‡) JARID1A/KDM5A (†,‡) Demethylases: Demethylases:
PHF8/KDM7B (+,†) JHDM1D/KDM7A (+,†) JMJD1A/KDM2A (+,†) ???
SUV39H2/KMT1B (†,‡) SET1B/KMT2G (+,†,‡)

For pathway key and background please visit:

www.cellsignal.com/histone
 Key Products for Histones and Histone Modifications
Visit www.cellsignal.com/histonelist for full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY

40
#12349 Histone H2A (D6O3A) Rabbit mAb WB, IF-IC, ChIP H, M, R, Mk, Z, (Hm, B, Dg) Ubiquityl-Histone H2A (Lys119)
35 (D27C4) XP Rabbit mAb #8240
®

% of total input chromatin

#2576 Acetyl-Histone H2A (Lys5) Antibody WB, IP, IHC-P H, M, R, Mk 30
Normal Rabbit
#8240 Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb WB, IP, ChIP H, M, R, Mk 25 IgG #2729
#7631 Histone H2A.X (D17A3) XP® Rabbit mAb WB, IHC-P, IF-IC H, M, R, Mk 20
15
#9718 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Mk
10
#2718 Histone H2A.Z Antibody WB, IP, IF-IC H, M, R, Mk, Z, (C, X, B) 5
#12364 Histone H2B (D2H6) Rabbit mAb WB, IHC-P, ChIP H, M, R, Mk, (C, Z, B) 0
HoxA1 HoxA2 GAPDH α Satellite
#12799 Acetyl-Histone H2B (Lys5) (D5H1S) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, (Hm, C, Z, B, Hr)
Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb
#9083 Acetyl-Histone H2B (Lys15) (D8H1) XP® Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk, (Z, B, Pg, Hr) #8240: Chromatin IPs were performed with cross-linked chromatin
#5546 Ubiquityl-Histone H2B (Lys120) (D11) XP® Rabbit mAb WB, ChIP H, M, R, Mk from 4 x 106 NCCIT cells and either 10 µl of #8240 or 2 µl of
Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin
#4499 Histone H3 (D1H2) XP® Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Mk, (Hm, C, Dm, X, Z, B) IP Kit (Magnetic Beads) #9003 and primers to the designated loci.
#4620 Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) ChIP H, M, (R, Hm, C, Dm, X, Z, B)
#9706 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb WB, IF-F, IF-IC, F H, M, R
#9701 Phospho-Histone H3 (Ser10) Antibody WB, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Mk, Dm, Z, Sc, (X) 45
40 Acetyl-Histone H3 (Lys9)
#9713 Phospho-Histone H3 (Ser28) Antibody WB, IP, IF-F, IF-IC, F H, M, Hm, Dm, (R, C, X, Z, B)

% of total input chromatin

35 (C5B11) Rabbit mAb #9649
#9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP H, M, R, Mk, Z, Ce, (Sc) 30
Normal Rabbit
25 IgG #2729
#9677 Acetyl-Histone H3 (Lys9/Lys14) Antibody WB, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, Ce, (Z)
20
#7627 Acetyl-Histone H3 (Lys14) (D4B9) Rabbit mAb WB, IP, ChIP H, M, R, Mk, (Hm, Dm, X, Z, Pg, Sc, Hr) 15
#13998 Acetyl-Histone H3 (Lys18) (D8Z5H) Rabbit mAb WB, IP, IHC-P, F, ChIP H, M, R, Mk, Sc, (Hm, Pg) 10
5
#8173 Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb WB, IF-IC, ChIP H, M, R, Mk, (Hm, X, Z, Hr) 0
RPL30 GAPDH MyoD1 α Satellite
#5326 Mono-Methyl-Histone H3 (Lys4) (D1A9) XP Rabbit mAb ®
WB, IF-IC, ChIP H, M, R, Mk, (Dm)
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649:
#14186 Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk, (X, Z, Pg, Sc, Hr) Chromatin IPs were performed with cross-linked chromatin from
#14111 Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb WB, IP, ChIP H, M, R, Mk, (Hm, B) 4 x 106 HeLa cells and either 10 μl of #9649 or 2 μl of Normal
Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit
#12522 Mono-Methyl-Histone H3 (Lys79) (D5X1S) Rabbit mAb WB, ChIP H, M, R, Mk
(Magnetic Beads) #9003 and primers to the designated loci.
#9725 Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb WB, IP, IHC-P, IF-IC, ChIP H, M, R, Mk
#4658 Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk, (Dm, X, Z, B, Pg, Sc, Ce)
#9728 Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb WB, IP, IF-IC, F, ChIP H, M, R, Mk A 20
Tri-Methyl-Histone H3 (Lys27)
18 (C36B11) Rabbit mAb #9733
#2901 Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb WB, IHC-P, IF-IC H, M, R, Mk
16
#5427 Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb WB, ChIP H, M, R, Mk Normal Rabbit
% of total input chromatin 14 IgG #2729
#5327 Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb WB, IP, IF-IC, ChIP H, M, R, Mk 12
#9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb WB, IHC-P, IF-IC, F, ChIP H, M, R, Mk, Dm, Sc, (X, Z) 10
#13969 Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk, (B) 8

#9733 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP H, M, R, Mk, (X, Z) 6
4
#4909 Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb WB, ChIP H, M, R, Mk, (Hm, C, Dm, X, Z, B)
2
#9763 Tri-Methyl-Histone H3 (Lys36) Antibody WB, IHC-P, IF-IC H, M, R, Mk
0
GAPDH RPL30 MyoD1 MYT-1
#4473 Pan-Methyl-Histone H3 (Lys9) (D54) XP Rabbit mAb ®
WB, IP, IF-IC, F, ChIP H, M, R, Mk, (C, Dm, X, Z, B, Pg, Sc, Ce)
#13919 Histone H4 (D2X4V) Rabbit mAb WB, IHC-P, IF-IC H, M, R, Mk, (Hm, C, B) B
#8647 Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb WB, IP, IHC-P, IF-IC, ChIP H, M, R, Mk, (C, Dm, X, Z, B, Pg, Hr, Ce)
#2594 Acetyl-Histone H4 (Lys8) Antibody WB H, M, R, Mk, (Ce)
#13944 Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb WB, IP, IF-IC, F, ChIP H, R, Mk, (C, Dm)
#13534 Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk
#9724 Mono-Methyl-Histone H4 (Lys20) Antibody WB H, M, R, Mk, (Dm, X, Z, B, Pg, Hr, Ce)
#5737 Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb WB, ChIP H, M, R, Mk, (X, B, Pg)

RELATED KITS AND ASSAYS

#9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
#9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) Tri-Methyl Histone H3 (Lys27) (C36B11) Rabbit mAb #9733:
Chromatin IPs (A) were performed with cross-linked chromatin
#7253 PathScan® Total Histone H3 Sandwich ELISA Kit
from 4 x 106 HeLa cells and either 10 µl of #9733 or 2 µl of
#7866 PathScan® Tri-Methyl-Histone H3 (Lys27) Sandwich ELISA Kit Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin
#9927 Acetyl-Histone H3 Antibody Sampler Kit IP Kit (Magnetic Beads) #9003 and primers to the designated loci.
IF analysis (B) of HeLa cells using #9733 (green). Actin filaments
15FLYHISM_REFM099ENG_00

have been labeled with DyLight™ 554 Phalloidin #13054 (red).

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry
/ -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all
species expected / ( ) 100% sequence homology

© 2015 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, PathScan, SimpleChIP, and XP are trademarks of Cell Signaling Technology, Inc. Patent No. 5,675,063. All other trademarks are the property of their respective owners.

For Research Use Only. Not For Use In Diagnostic Procedures.

www.cellsignal.com/histone