Biological Effects of the Vibe Machine on Human DNA in-vitro Glen Rein, Ph.D. ‘Quantum Biology Research Lab Dix Hillls, NY Peter Moscow, Ph.D. Irene Mosvold F.EMA. Holistic Consultants Louisville, KY Introduction: The Vibe machine is currently being investigated for its biomedical healing effects. The Vibe generates plasma waves since its inert gases are electrically activated by an EM pulse. Plasma waves generated by Priore were efficacious in treating a variety of animal diseases including cancer (Riviere, 1964). Plasma waves were also used by Rife and reported to treat a variety of diseases as well as to kill microorganisms in-vitro (Rife, 1953). Several commercially available plasma generating devices are now on the market, but relatively few have been tested using contemporary scientific methods. Plasma waves can be considered a type of non-classical EM field (Rein, 2004), because they have properties that can not be explained by Maxwell's equations. Some evidence indicates that classical EM fields of the same frequency, generated in the absence of a plasma tube, are significantly less biologically active (Bare, 1997). Other forms of non-classical EM fields have also been shown to be biologically active using both in-vitro and in-vivo test methods (Rein, 2001). The Quantum Biology Research Lab utilizes in-vitro biological assays to measure the biological effects of non-classical EM fields generated from both subtle energy devices (Rein, 1998) and healers (Rein Laskow). Of the various methods investigated it was discovered that DNA is particularly sensitive to non-classical EM fields. Thus, in addition to classical EM fields (Semin, 1995), non-classic EM fields resonate with DNA producing conformational changes (winding and unwinding) in the secondary structure of the double-helix (Rein, 1994a, 1995, 1996). More recently a highly-sensitive DNA rewinding assay was developed taking dynamic measurements (every 10 seconds) after prior denaturation (separation) of the two strands making up the DNA helix. Using the new assay method it was demonstrated that bio-eneray from healing arts practitioners slowed down the rate of rewinding (Rein, 2003). It was therefore of interest to determine whetherthe energy fields generated from the Vibe machine could resonate with human DNA and have similar effects to healing arts practitioners. Goals of this Research Project 1.Obtain scientific evidence that the Vibe can produce measurable and reproducible biological effects on DNA using state-of-the-art scientific methodology. 2. Compare the effects of the Vibe on DNA with those obtained using the same assay with healing arts practitioners. 3. Compare in real-time physical measures of DNA rewinding with energetic measures using a radionics device The Experimental Approach In these experiments the biological system being influenced by the EM fields from the Vibe is purified human DNA suspended in a natural ionic environment. Utilizing the well known fact that heat shock causes DNA strands to unwind (Marmur, 1961), the Quantum Biology Research Lab developed a sensitive assay which involves measuring the kinetics of rewinding following heat shock. It is well known that heat causes unwinding of the two strands of the double-helix (Thomas, 1995). As the DNA cools, it recovers by rewinding back into an intact double helix (Marmur, 1961). This renaturation process of rewinding can be monitored by measuring the absorption of UV light as a function of the cooling temperature or increasing time (Thomas, 195). Since the absorption of light decreases as DNA rewinds, a curve is obtained with a negative slope. The larger the negative number of the slope, the faster the DNA rewinds. During the rewinding process, hydrogen bonds reform to connect the two strands. Thus DNA rewinding is directly related to the number of hydrogen bonds. Since the quantum properties of the hydrogen bond have been previously described (Tuckerman et al, 1977), rewinding of DNA can be considered a measure of the quantum properties of DNA. Experimental Methods Three types of experiments were conducted in this study. The control experiments were done first in the presence of ambient EM fields! but in the absence of any man-made EM fields. Vibe experiments involved exposing DNA to the Vibe's energy field at the very end of the heat-induced unwinding. The third set of experiments correlated typical rewinding as measured in a spectrophotometer with energetic measures at a distance using radionic equipment. The specific protocol that was followed involved making a stock solution (0.4mg/mi) of human placental DNA (Sigma Chemical Co., St. Louis) in deionized water. The stock solution was diluted to 0.03mg/ml in water and heat shocked (80°C for five minutes). Immediately after heat treatment the DNA wasgently transferred to a quartz cuvette and then placed inside the spectrophotometer. For Vibe treatment experiments, the beaker containing hot water and DNA was placed four feet from the Vibe machine at a height corresponding to the level of the inert gas tubes. The Vibe was switched on for one or two 30 second cycles at the end of the 5 minute heating period. Then the sample was immediately transferred to the spectrophotometer to begin the rewinding measurements. Control experiments followed the same procedure in the absence of a Vibe treatment. A third set of experiments were done in collaboration with Peter Moscow and Irene Mosvold. Nine aliquots (small samples) of the DNA stock solution were transferred to 9 separate tested tubes and diluted with water as described above. Each sample was labeled 1-9 and photographed. The samples remained in New York and the photographs were sent to the HPC Ltd Clinic in Kentucky. The photographs were used as a witness to energetically connect to the physical DNA samples which were being heat shocked in NY. In addition a live telephone line was used to connect the two experiments in real time. Peter began the radionic measurements using the Harmonic Translator at time zero when rewinding of the physical DNA began. In this way radionic and spectrophotometric measurements were synchronized in real time. For all experiments, the conformation of DNA was measured with state-of-the art equipment using a UV-visible diode array spectrophotometer (Hewlett Packard 8451A) to quantify the amount of UV light absorbed (Thomas, 1995). Absorption measurements were taken at 260nm every 10 seconds over the course of thirty minutes. This rewinding curves were used for both quantitative (see below) and qualitative analysis. Qualitative analysis was also done by obtaining pattern information from the dynamic behavior over longer time periods. Quantitative analysis of the data involved measuring the initial slope during the first few minutes of rewinding. The slope in this region corresponds to the initial recovery rate, classically used by biochemists in studying enzyme kinetics. The slopes were calculated using IBM Excel software. The light grey irregular line in Figure 1 is a plot of the raw absorption data collected by the spectrophotometer as a function of time after heat shock. The solid black line is the computer generated best-fit calculation of the slope. The slope was calculated for each separate experiment and then compared statistically using a two sample t-test, (assuming equal variance). For statistical analyses of this data a total of 10 control experiments, 4 Vibe experiments and 6 control experiments, were used.