RAW DATA (enter the pH of the buffers used in the experiments)

Wavelength buffer 1 buffer 2 buffer 3 buffer 4

 Normalized to (λ = nm):
(copy and paste the data for the wavelength you want to mormalize to)

NORMALIZED DATA
Wavelength buffer 1 buffer 2 buffer 3 buffer 4
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
 0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0

SPECTRAL DIFFERENCE PLOT (determine the analytical wavelengths - maximum positive

Wavelength buffer 1 buffer 2 buffer 3 buffer 4
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0
0 0 0 0 0

(copy and paste the data for the analytical wavelengths)

pH λmax λmin Absolute(λmax)

buffer 1 0
buffer 2 0
buffer 3 0
buffer 4 0
buffer 5 0
buffer 6 0
buffer 7 0
buffer 8 0
buffer 9 0
buffer 10 0
buffer 11 0
buffer 12 0
periments)
buffer 5 buffer 6 buffer 7 buffer 8 buffer 9 buffer 10

Paste the UV data here

buffer 5 buffer 6 buffer 7 buffer 8 buffer 9 buffer 10
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
 0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0

engths - maximum positive and negative deviations - graphically)

buffer 5 buffer 6 buffer 7 buffer 8 buffer 9 buffer 10
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
 0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0

Absorbance difference
wavelengths)

Absolute(λmin) Sum = total absorbance

0 0
Total absorbance difference vs pH
0 0 pKa Determination
0 0
0 0 3
0 0
0 0 2
0 0
0 0
1
0 0
0 0
0 0 0
0 0 2 5 8 11
pH
buffer 11 buffer 12 Citation:

Substituent effect on the basicity (pKa) of aryl guanidi

C. Dardonville, B.A. Caine, M. Navarro de la Fuente, G.
New J. Chem. 2017, DOI: 10.1039/C7NJ02497E

Reference:
"Rapid Determination of Ionization Constants (pKa) by
C.H. Ríos Martínez, C. Dardonville
ACS Med. Chem. Lett.. 2013, 4, 142-145.
http://pubs.acs.org/articlesonrequest/AOR-TV3vU6DJ3
http://hdl.handle.net/10261/82654

Raw dat

12

10
Absorbance

2
4

0
250 300 350
Waveleng
buffer 11 buffer 12
0 0
0 0
0 0
0 0
0 0
0 0
0 0
Norm
0 0
0 0
12
0 0
0 0
0 0
0 0
10
0 0
Absorbance

0 0
0 0
0 0 8
0 0
0 0
0 0
0 0 6
0 0
0 0
0 0
0 0 4
0 0
0 0
0 0
0 0 2
0 0
0 0
0 0
0 0 0
0 0 250 300 350
 0
250 300 350
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
 0 0
0 0
0 0
0 0
0 0
0 0

buffer 11 buffer 12
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0 Spectral dif
0 0
0 0
0 0 3.5
0 0
0 0 3
0 0
0 0 2.5
0 0
0 0 2
Absorbance

0 0
0 0 1.5
0 0
0 0 1
0 0
0 0 0.5
0 0
0 0 0
0 0
0 0 -0.5
0 0
-1

-1.5
 0

-0.5

0 0 -1
0 0
0 0 -1.5
0 0
0 0 -2
0 0
0 0 -2.5
0 0
0 0 -3
0 0
0 0 -3.5
250 270 290 310 330 350 370
0 0
0 0 Waveleng
318
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
 0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0

bsorbance difference vs pH
pKa Determination

5 8 11 14
pH
y (pKa) of aryl guanidines and 2-(arylimino)imidazolidines: correlations of pH-metric and UV
arro de la Fuente, G. Martín Herranz, B. Corrales Mariblanca, P. L. A. Popelier
/C7NJ02497E

n Constants (pKa) by UV Spectroscopy Using 96-Well Microtiter Plates”

uest/AOR-TV3vU6DJ386KArNmw9jF

Raw data
350 400 450 500
Wavelength (nm)
Normalized data

350 400 450 500

Wavelength (nm)
350 400 450 500
Wavelength (nm)
Spectral difference plot

Column
B

Column
C

Column
D

Column
E

Column
F

Column
G

Column
H

Column
I

Column
J

Column
K

Column
 Column
H

Column
I

Column
J

Column
K

Column
L

Column
M

330 350 370 390 410 430 450 470 490

Wavelength (nm)
ons of pH-metric and UV-metric values with predictions from gas-phase ab initio bond lengt
Popelier
500
500
Column
B

Column
C

Column
D

Column
E

Column
F

Column
G

Column
H

Column
I

Column
J

Column
K

Column
 Column
H

Column
I

Column
J

Column
K

Column
L

Column
M

490
initio bond lengths
RAW DATA (pH of buffer solutions used in the experiment)
Wavelength 2.68 5.09 6.34 6.8
230 0.82695 0.80767 0.82671 0.90198
232 1.12480 1.14879 1.03912 1.05687
234 1.16201 1.15358 1.10788 0.99585
236 1.15271 1.10659 1.11211 0.98860
238 1.13032 1.02602 1.07619 0.96851
240 1.07511 0.95419 1.02150 0.96331
242 1.01048 0.91548 0.95313 0.92803
244 0.95152 0.88226 0.90023 0.88983
246 0.85931 0.79934 0.81676 0.82251
248 0.80954 0.76093 0.77135 0.78414
250 0.75468 0.72052 0.71405 0.74027
252 0.67688 0.64988 0.64401 0.67513
254 0.64472 0.62793 0.61167 0.65108
256 0.60512 0.59010 0.57197 0.60954
258 0.57903 0.56956 0.54652 0.58300
260 0.57415 0.56885 0.54160 0.57465
262 0.57715 0.57474 0.54124 0.57350
264 0.59316 0.59424 0.55632 0.58750
266 0.62044 0.61828 0.57831 0.60745
268 0.64492 0.64107 0.59974 0.62879
270 0.67525 0.67189 0.62573 0.65287
272 0.70231 0.69671 0.64886 0.67617
274 0.72416 0.71658 0.66767 0.69599
276 0.74843 0.73670 0.68534 0.71571
278 0.76875 0.76062 0.70612 0.73480
280 0.79163 0.77846 0.72397 0.75311
282 0.80491 0.79102 0.73642 0.76653
284 0.81677 0.80342 0.74647 0.77819
286 0.82649 0.81176 0.75340 0.78479
288 0.82964 0.81613 0.75730 0.78956
290 0.83268 0.82056 0.76088 0.79321
292 0.84456 0.81912 0.76002 0.79476
294 0.83120 0.81595 0.75826 0.79417
296 0.82782 0.81452 0.75530 0.79265
298 0.82343 0.81081 0.75158 0.78984
300 0.81653 0.80379 0.74608 0.78468
302 0.80709 0.79609 0.73868 0.77739
304 0.79488 0.78525 0.72830 0.76891
306 0.78311 0.77498 0.71838 0.75877
308 0.76794 0.76030 0.71024 0.74672
310 0.75049 0.74457 0.69230 0.73254
312 0.73310 0.72663 0.67655 0.71882
314 0.71214 0.70976 0.65869 0.70205
316 0.68849 0.68503 0.63766 0.68129
318 0.66615 0.66510 0.61990 0.66374
320 0.64328 0.64279 0.60051 0.64340
322 0.61621 0.61658 0.57498 0.61937
324 0.59234 0.59465 0.55533 0.59866
326 0.56708 0.56703 0.53278 0.57780
328 0.54460 0.54400 0.51254 0.55585
330 0.52348 0.52243 0.49243 0.53754
332 0.49729 0.49738 0.46932 0.51438
334 0.47547 0.47517 0.44879 0.49458
336 0.45346 0.45104 0.42871 0.47501
338 0.43038 0.42906 0.40609 0.45299
340 0.40942 0.40740 0.38828 0.43607
342 0.38903 0.38660 0.37067 0.41762
344 0.36706 0.36575 0.35100 0.39751
346 0.33914 0.34046 0.32742 0.37433
348 0.32135 0.32191 0.31189 0.35926
350 0.30397 0.30363 0.29371 0.34277
352 0.28741 0.28779 0.28106 0.33075
354 0.26742 0.26908 0.26498 0.31412
356 0.25276 0.25501 0.25076 0.30102
358 0.23788 0.24024 0.23721 0.28941
360 0.22036 0.22197 0.22327 0.27399
362 0.20668 0.20951 0.21190 0.26347
364 0.19236 0.19644 0.19842 0.25223
366 0.17642 0.18066 0.18423 0.23702
368 0.16425 0.16914 0.17385 0.22605
370 0.15010 0.15336 0.16165 0.21373
372 0.14074 0.14347 0.15192 0.20394
374 0.12716 0.13438 0.14057 0.19428
376 0.11720 0.12280 0.13258 0.18531
378 0.10792 0.11336 0.12390 0.17618
380 0.09847 0.10280 0.11402 0.16523
382 0.08814 0.09517 0.10529 0.15655
384 0.08115 0.08561 0.09751 0.14893
386 0.08135 0.07756 0.08988 0.13980
388 0.06569 0.07220 0.08366 0.13114
390 0.05711 0.06414 0.07652 0.12313
392 0.05220 0.05819 0.07019 0.11685
394 0.04495 0.05134 0.06395 0.10920
396 0.04420 0.04560 0.05837 0.10329
398 0.03561 0.04058 0.05383 0.09601
400 0.03035 0.03439 0.04840 0.08868
402 0.02631 0.03131 0.04493 0.08266
404 0.02248 0.02719 0.03928 0.07697
406 0.01946 0.02483 0.03580 0.07275
408 0.01689 0.02071 0.03204 0.06664
410 0.02991 0.01864 0.02832 0.06235
412 0.01314 0.01666 0.02584 0.05845
414 0.01232 0.01490 0.02311 0.05391
416 0.00969 0.01396 0.02085 0.05061
418 0.00829 0.01244 0.01827 0.04592
420 0.00787 0.01107 0.01741 0.04365
422 0.00711 0.00986 0.01473 0.03925
424 0.00639 0.00972 0.01304 0.03737
426 0.00750 0.00926 0.01230 0.03396
428 0.00692 0.00892 0.01114 0.03185
430 0.00585 0.00826 0.00897 0.02969
432 0.00523 0.00759 0.00844 0.02675
434 0.00471 0.00713 0.00698 0.02535
436 0.00446 0.00695 0.00534 0.02364
438 0.00436 0.00641 0.00216 0.02103
440 0.00411 0.00574 0.00184 0.01905
442 0.00344 0.00511 -0.00056 0.01676
444 0.00397 0.00517 -0.00073 0.01598
446 0.00397 0.00458 -0.00243 0.01426
448 0.00376 0.00526 -0.00294 0.01322
450 0.00355 0.00443 -0.00347 0.01145
452 0.00394 0.00386 -0.00358 0.01040
454 0.00312 0.00406 -0.00510 0.00880
456 0.00340 0.00377 -0.00448 0.00870
458 0.00315 0.00340 -0.00577 0.00711
460 0.00294 0.00355 -0.00799 0.00639
462 0.00341 0.00318 -0.00668 0.00615
464 0.00273 0.00321 -0.00651 0.00475
466 0.00310 0.00260 -0.00804 0.00450
468 0.00304 0.00241 -0.00895 0.00423
470 0.00235 0.00199 -0.00877 0.00353
472 0.00272 0.00248 -0.00956 0.00409
474 0.00201 0.00193 -0.00800 0.00269
476 0.00310 0.00338 -0.00347 0.00437
478 0.00290 0.00269 -0.00383 0.00311
480 0.01830 0.00186 -0.00338 0.00307
482 0.00312 0.00259 -0.00394 0.00327
484 0.00349 0.00196 -0.00373 0.00273
486 0.00735 0.00225 -0.00209 0.00266
488 0.00494 0.00181 -0.00421 0.00228
490 0.00318 0.00206 -0.00385 0.00192
492 0.00288 0.00224 -0.00418 0.00169
494 0.01847 0.00201 -0.00357 0.00259
496 0.01158 0.00158 -0.00449 0.00134
498 0.00138 0.00150 -0.00444 0.00155
500 0.00332 0.00148 -0.00445 0.00120
 500 0.00332 0.00148 -0.00445 0.00120
(normalized to zero for λ = 500 nm)

NORMALIZED DATA
Wavelength 2.68 5.09 6.34 6.8
230 0.82363 0.80619 0.83116 0.90078
232 1.12148 1.14731 1.04357 1.05567
234 1.15869 1.1521 1.11233 0.99465
236 1.14939 1.10511 1.11656 0.9874
238 1.127 1.02454 1.08064 0.96731
240 1.07179 0.95271 1.02595 0.96211
242 1.00716 0.914 0.95758 0.92683
244 0.9482 0.88078 0.90468 0.88863
246 0.85599 0.79786 0.82121 0.82131
248 0.80622 0.75945 0.7758 0.78294
250 0.75136 0.71904 0.7185 0.73907
252 0.67356 0.6484 0.64846 0.67393
254 0.6414 0.62645 0.61612 0.64988
256 0.6018 0.58862 0.57642 0.60834
258 0.57571 0.56808 0.55097 0.5818
260 0.57083 0.56737 0.54605 0.57345
262 0.57383 0.57326 0.54569 0.5723
264 0.58984 0.59276 0.56077 0.5863
266 0.61712 0.6168 0.58276 0.60625
268 0.6416 0.63959 0.60419 0.62759
270 0.67193 0.67041 0.63018 0.65167
272 0.69899 0.69523 0.65331 0.67497
274 0.72084 0.7151 0.67212 0.69479
276 0.74511 0.73522 0.68979 0.71451
278 0.76543 0.75914 0.71057 0.7336
280 0.78831 0.77698 0.72842 0.75191
282 0.80159 0.78954 0.74087 0.76533
284 0.81345 0.80194 0.75092 0.77699
286 0.82317 0.81028 0.75785 0.78359
288 0.82632 0.81465 0.76175 0.78836
290 0.82936 0.81908 0.76533 0.79201
292 0.84124 0.81764 0.76447 0.79356
294 0.82788 0.81447 0.76271 0.79297
296 0.8245 0.81304 0.75975 0.79145
298 0.82011 0.80933 0.75603 0.78864
300 0.81321 0.80231 0.75053 0.78348
302 0.80377 0.79461 0.74313 0.77619
304 0.79156 0.78377 0.73275 0.76771
306 0.77979 0.7735 0.72283 0.75757
308 0.76462 0.75882 0.71469 0.74552
310 0.74717 0.74309 0.69675 0.73134
312 0.72978 0.72515 0.681 0.71762
314 0.70882 0.70828 0.66314 0.70085
316 0.68517 0.68355 0.64211 0.68009
318 0.66283 0.66362 0.62435 0.66254
320 0.63996 0.64131 0.60496 0.6422
322 0.61289 0.6151 0.57943 0.61817
324 0.58902 0.59317 0.55978 0.59746
326 0.56376 0.56555 0.53723 0.5766
328 0.54128 0.54252 0.51699 0.55465
330 0.52016 0.52095 0.49688 0.53634
332 0.49397 0.4959 0.47377 0.51318
334 0.47215 0.47369 0.45324 0.49338
336 0.45014 0.44956 0.43316 0.47381
338 0.42706 0.42758 0.41054 0.45179
340 0.4061 0.40592 0.39273 0.43487
342 0.38571 0.38512 0.37512 0.41642
344 0.36374 0.36427 0.35545 0.39631
346 0.33582 0.33898 0.33187 0.37313
348 0.31803 0.32043 0.31634 0.35806
350 0.30065 0.30215 0.29816 0.34157
352 0.28409 0.28631 0.28551 0.32955
354 0.2641 0.2676 0.26943 0.31292
356 0.24944 0.25353 0.25521 0.29982
358 0.23456 0.23876 0.24166 0.28821
360 0.21704 0.22049 0.22772 0.27279
362 0.20336 0.20803 0.21635 0.26227
364 0.18904 0.19496 0.20287 0.25103
366 0.1731 0.17918 0.18868 0.23582
368 0.16093 0.16766 0.1783 0.22485
370 0.14678 0.15188 0.1661 0.21253
372 0.13742 0.14199 0.15637 0.20274
374 0.12384 0.1329 0.14502 0.19308
376 0.11388 0.12132 0.13703 0.18411
378 0.1046 0.11188 0.12835 0.17498
380 0.09515 0.10132 0.11847 0.16403
382 0.08482 0.09369 0.10974 0.15535
384 0.07783 0.08413 0.10196 0.14773
386 0.07803 0.07608 0.09433 0.1386
388 0.06237 0.07072 0.08811 0.12994
390 0.05379 0.06266 0.08097 0.12193
392 0.04888 0.05671 0.07464 0.11565
394 0.04163 0.04986 0.0684 0.108
396 0.04088 0.04412 0.06282 0.10209
398 0.03229 0.0391 0.05828 0.09481
400 0.02703 0.03291 0.05285 0.08748
402 0.02299 0.02983 0.04938 0.08146
404 0.01916 0.02571 0.04373 0.07577
406 0.01614 0.02335 0.04025 0.07155
408 0.01357 0.01923 0.03649 0.06544
410 0.02659 0.01716 0.03277 0.06115
412 0.00982 0.01518 0.03029 0.05725
414 0.009 0.01342 0.02756 0.05271
416 0.00637 0.01248 0.0253 0.04941
418 0.00497 0.01096 0.02272 0.04472
420 0.00455 0.00959 0.02186 0.04245
422 0.00379 0.00838 0.01918 0.03805
424 0.00307 0.00824 0.01749 0.03617
426 0.00418 0.00778 0.01675 0.03276
428 0.0036 0.00744 0.01559 0.03065
430 0.00253 0.00678 0.01342 0.02849
432 0.00191 0.00611 0.01289 0.02555
434 0.00139 0.00565 0.01143 0.02415
436 0.00114 0.00547 0.00979 0.02244
438 0.00104 0.00493 0.00661 0.01983
440 0.00079 0.00426 0.00629 0.01785
442 0.00012 0.00363 0.00389 0.01556
444 0.00065 0.00369 0.00372 0.01478
446 0.00065 0.0031 0.00202 0.01306
448 0.00044 0.00378 0.00151 0.01202
450 0.00023 0.00295 0.00098 0.01025
452 0.00062 0.00238 0.00087 0.0092
454 -0.0002 0.00258 -0.00065 0.0076
456 0.00008 0.00229 -0.00003 0.0075
458 -0.00017 0.00192 -0.00132 0.00591
460 -0.00038 0.00207 -0.00354 0.00519
462 0.00009 0.0017 -0.00223 0.00495
464 -0.00059 0.00173 -0.00206 0.00355
466 -0.00022 0.00112 -0.00359 0.0033
468 -0.00028 0.00093 -0.0045 0.00303
470 -0.00097 0.00051 -0.00432 0.00233
472 -0.0006 0.001 -0.00511 0.00289
474 -0.00131 0.00045 -0.00355 0.00149
476 -0.00022 0.0019 0.00098 0.00317
478 -0.00042 0.00121 0.00062 0.00191
480 0.01498 0.00038 0.00107 0.00187
482 -0.0002 0.00111 0.00051 0.00207
484 0.00017 0.00048 0.00072 0.00153
486 0.00403 0.00077 0.00236 0.00146
488 0.00162 0.00033 0.00024 0.00108
490 -0.00014 0.00058 0.0006 0.00072
492 -0.00044 0.00076 0.00027 0.00049
494 0.01515 0.00053 0.00088 0.00139
496 0.00826 0.0001 -0.00004 0.00014
498 -0.00194 0.00002 0.00001 0.00035
 500 0 0 0 0

SPECTRAL DIFFERENCE PLOT (the analytical wavelengths determined graphically are

Wavelength 2.68 5.09 6.34 6.8
230 0 -0.01744 0.00753 0.07715
232 0 0.02583 -0.07791 -0.06581
234 0 -0.00659 -0.04636 -0.16404
236 0 -0.04428 -0.03283 -0.16199
238 0 -0.10246 -0.04636 -0.15969
240 0 -0.11908 -0.04584 -0.10968
242 0 -0.09316 -0.04958 -0.08033
244 0 -0.06742 -0.04352 -0.05957
246 0 -0.05813 -0.03478 -0.03468
248 0 -0.04677 -0.03042 -0.02328
250 0 -0.03232 -0.03286 -0.01229
252 0 -0.02516 -0.0251 0.00037
254 0 -0.01495 -0.02528 0.00848
256 0 -0.01318 -0.02538 0.00654
258 0 -0.00763 -0.02474 0.00609
260 0 -0.00346 -0.02478 0.00262
262 0 -0.00057 -0.02814 -0.00153
264 0 0.00292 -0.02907 -0.00354
266 0 -0.00032 -0.03436 -0.01087
268 0 -0.00201 -0.03741 -0.01401
270 0 -0.00152 -0.04175 -0.02026
272 0 -0.00376 -0.04568 -0.02402
274 0 -0.00574 -0.04872 -0.02605
276 0 -0.00989 -0.05532 -0.0306
278 0 -0.00629 -0.05486 -0.03183
280 0 -0.01133 -0.05989 -0.0364
282 0 -0.01205 -0.06072 -0.03626
284 0 -0.01151 -0.06253 -0.03646
286 0 -0.01289 -0.06532 -0.03958
288 0 -0.01167 -0.06457 -0.03796
290 0 -0.01028 -0.06403 -0.03735
292 0 -0.0236 -0.07677 -0.04768
294 0 -0.01341 -0.06517 -0.03491
296 0 -0.01146 -0.06475 -0.03305
298 0 -0.01078 -0.06408 -0.03147
300 0 -0.0109 -0.06268 -0.02973
302 0 -0.00916 -0.06064 -0.02758
304 0 -0.00779 -0.05881 -0.02385
306 0 -0.00629 -0.05696 -0.02222
308 0 -0.0058 -0.04993 -0.0191
310 0 -0.00408 -0.05042 -0.01583
312 0 -0.00463 -0.04878 -0.01216
314 0 -0.00054 -0.04568 -0.00797
316 0 -0.00162 -0.04306 -0.00508
318 0 0.00079 -0.03848 -0.00029
320 0 0.00135 -0.035 0.00224
322 0 0.00221 -0.03346 0.00528
324 0 0.00415 -0.02924 0.00844
326 0 0.00179 -0.02653 0.01284
328 0 0.00124 -0.02429 0.01337
330 0 0.00079 -0.02328 0.01618
332 0 0.00193 -0.0202 0.01921
334 0 0.00154 -0.01891 0.02123
336 0 -0.00058 -0.01698 0.02367
338 0 0.00052 -0.01652 0.02473
340 0 -0.00018 -0.01337 0.02877
342 0 -0.00059 -0.01059 0.03071
344 0 0.00053 -0.00829 0.03257
346 0 0.00316 -0.00395 0.03731
348 0 0.0024 -0.00169 0.04003
350 0 0.0015 -0.00249 0.04092
352 0 0.00222 0.00142 0.04546
354 0 0.0035 0.00533 0.04882
356 0 0.00409 0.00577 0.05038
358 0 0.0042 0.0071 0.05365
360 0 0.00345 0.01068 0.05575
362 0 0.00467 0.01299 0.05891
364 0 0.00592 0.01383 0.06199
366 0 0.00608 0.01558 0.06272
368 0 0.00673 0.01737 0.06392
370 0 0.0051 0.01932 0.06575
372 0 0.00457 0.01895 0.06532
374 0 0.00906 0.02118 0.06924
376 0 0.00744 0.02315 0.07023
378 0 0.00728 0.02375 0.07038
380 0 0.00617 0.02332 0.06888
382 0 0.00887 0.02492 0.07053
384 0 0.0063 0.02413 0.0699
386 0 -0.00195 0.0163 0.06057
388 0 0.00835 0.02574 0.06757
390 0 0.00887 0.02718 0.06814
392 0 0.00783 0.02576 0.06677
394 0 0.00823 0.02677 0.06637
396 0 0.00324 0.02194 0.06121
398 0 0.00681 0.02599 0.06252
400 0 0.00588 0.02582 0.06045
402 0 0.00684 0.02639 0.05847
404 0 0.00655 0.02457 0.05661
406 0 0.00721 0.02411 0.05541
408 0 0.00566 0.02292 0.05187
410 0 -0.00943 0.00618 0.03456
412 0 0.00536 0.02047 0.04743
414 0 0.00442 0.01856 0.04371
416 0 0.00611 0.01893 0.04304
418 0 0.00599 0.01775 0.03975
420 0 0.00504 0.01731 0.0379
422 0 0.00459 0.01539 0.03426
424 0 0.00517 0.01442 0.0331
426 0 0.0036 0.01257 0.02858
428 0 0.00384 0.01199 0.02705
430 0 0.00425 0.01089 0.02596
432 0 0.0042 0.01098 0.02364
434 0 0.00426 0.01004 0.02276
436 0 0.00433 0.00865 0.0213
438 0 0.00389 0.00557 0.01879
440 0 0.00347 0.0055 0.01706
442 0 0.00351 0.00377 0.01544
444 0 0.00304 0.00307 0.01413
446 0 0.00245 0.00137 0.01241
448 0 0.00334 0.00107 0.01158
450 0 0.00272 0.00075 0.01002
452 0 0.00176 0.00025 0.00858
454 0 0.00278 -0.00045 0.0078
456 0 0.00221 -0.00011 0.00742
458 0 0.00209 -0.00115 0.00608
460 0 0.00245 -0.00316 0.00557
462 0 0.00161 -0.00232 0.00486
464 0 0.00232 -0.00147 0.00414
466 0 0.00134 -0.00337 0.00352
468 0 0.00121 -0.00422 0.00331
470 0 0.00148 -0.00335 0.0033
472 0 0.0016 -0.00451 0.00349
474 0 0.00176 -0.00224 0.0028
476 0 0.00212 0.0012 0.00339
478 0 0.00163 0.00104 0.00233
480 0 -0.0146 -0.01391 -0.01311
482 0 0.00131 0.00071 0.00227
484 0 0.00031 0.00055 0.00136
486 0 -0.00326 -0.00167 -0.00257
 488 0 -0.00129 -0.00138 -0.00054
490 0 0.00072 0.00074 0.00086
492 0 0.0012 0.00071 0.00093
494 0 -0.01462 -0.01427 -0.01376
496 0 -0.00816 -0.0083 -0.00812
498 0 0.00196 0.00195 0.00229

500 0 0 0 0

Compound:
(N-(3-chloro-4-nitrophenyl)-4,5-dihydro-1H-imidazol-2-amine acetate (13a)

(copy and paste the data for the analytical wavelengths)

λmax λmin
pH 380 290 Absolute(λmax)
2.68 0 0 0
5.09 0.00617 -0.01028 0.00617
6.34 0.02332 -0.06403 0.02332
6.8 0.06888 -0.03735 0.06888
6.97 0.12959 -0.06683 0.12959
7.59 0.29281 -0.13109 0.29281
8.22 0.62671 -0.26764 0.62671
8.75 0.53767 -0.31873 0.53767
9.04 0.68606 -0.37121 0.68606
9.57 0.83339 -0.41787 0.83339
10.51 0.92646 -0.41661 0.92646
12.24 0.86648 -0.46358 0.86648
 6.97 7.59 8.22 8.75 9.04 9.57 10.51
1.02156 0.97215 0.65562 0.46754 0.56306 0.61867 0.79777
1.16293 1.05947 0.89756 0.61826 0.67269 0.80342 0.89136
1.19746 1.07541 0.97548 0.77128 0.82850 0.90064 0.94733
1.18291 1.09506 0.99588 0.83128 0.89936 0.93677 0.99586
1.14776 1.07754 1.01938 0.86730 0.93889 0.97206 1.02826
1.09599 1.05475 1.01864 0.88887 0.95800 0.98735 1.06042
1.02925 1.00965 1.00381 0.89009 0.95671 0.98863 1.07605
0.97981 0.97789 0.98334 0.88124 0.94455 0.97602 1.05679
0.88985 0.91767 0.94032 0.85119 0.91960 0.96225 1.01417
0.84270 0.88007 0.91182 0.83598 0.89563 0.94844 0.99910
0.78485 0.83434 0.87809 0.83137 0.86800 0.92248 0.96179
0.71385 0.77058 0.83020 0.77238 0.81750 0.88377 0.91145
0.67976 0.73977 0.78995 0.74307 0.78237 0.85049 0.88598
0.63659 0.69511 0.73297 0.70377 0.73482 0.80146 0.84179
0.60741 0.65938 0.68642 0.66741 0.69013 0.75227 0.79464
0.59272 0.63934 0.65086 0.63981 0.65675 0.71147 0.75611
0.58962 0.63124 0.63464 0.62797 0.64232 0.67836 0.73440
0.59437 0.62306 0.61039 0.60029 0.60834 0.62890 0.68070
0.60366 0.62281 0.59300 0.58070 0.58434 0.58722 0.63500
Paste the UV 0.62683
0.60839 data here 0.58437 0.56675 0.55762 0.55217 0.60038
0.62562 0.63466 0.57169 0.55066 0.53298 0.51278 0.55837
0.64483 0.64265 0.56638 0.53818 0.51435 0.48180 0.52645
0.66356 0.65097 0.55748 0.53028 0.50527 0.45818 0.49971
0.68306 0.65657 0.54557 0.51844 0.48430 0.43696 0.47195
0.70073 0.66458 0.53845 0.50766 0.47047 0.41973 0.44790
0.71234 0.67585 0.53813 0.50721 0.46360 0.41484 0.43182
0.72404 0.68220 0.53112 0.50299 0.45753 0.40992 0.41861
0.73881 0.68900 0.53021 0.50104 0.45526 0.40363 0.41167
0.74815 0.69444 0.52874 0.50255 0.45526 0.40622 0.40886
0.75746 0.69747 0.52745 0.52226 0.45808 0.41099 0.40965
0.76683 0.70406 0.54567 0.52625 0.46388 0.41768 0.41487
0.77420 0.70874 0.53431 0.51603 0.47047 0.42696 0.42232
0.78169 0.70928 0.53820 0.52031 0.47756 0.43535 0.43181
0.78089 0.71289 0.54361 0.52785 0.48603 0.44804 0.44421
0.77814 0.71489 0.54646 0.53485 0.49257 0.45565 0.45493
0.77488 0.71499 0.55476 0.54228 0.50289 0.46072 0.46723
0.77157 0.71278 0.55897 0.54521 0.51222 0.47200 0.48120
0.76365 0.71263 0.56626 0.55257 0.52230 0.48850 0.49703
0.75499 0.71188 0.57000 0.56564 0.52978 0.49962 0.51124
0.74465 0.70916 0.57568 0.56441 0.53845 0.51187 0.52630
0.73193 0.70529 0.58216 0.57019 0.54806 0.52762 0.54350
0.72320 0.69781 0.58614 0.57468 0.55626 0.54002 0.55634
0.70546 0.69194 0.58945 0.57889 0.56392 0.55384 0.57406
0.68814 0.68297 0.59528 0.58220 0.57486 0.57079 0.59374
0.67292 0.67514 0.59791 0.58582 0.58265 0.58423 0.60981
0.65417 0.66415 0.60111 0.58851 0.59177 0.59812 0.62545
0.63364 0.65411 0.60417 0.59322 0.60146 0.61563 0.64616
0.61550 0.64426 0.60830 0.59617 0.61020 0.63014 0.66370
0.59551 0.63252 0.61477 0.59918 0.62095 0.64702 0.67948
0.57765 0.62196 0.61736 0.60222 0.62878 0.66072 0.69810
0.56114 0.61408 0.62426 0.60776 0.64052 0.67940 0.71688
0.54015 0.60184 0.63114 0.61137 0.65152 0.69743 0.74180
0.52235 0.59115 0.63528 0.61116 0.66364 0.71271 0.75984
0.50564 0.58208 0.63930 0.61449 0.67376 0.72964 0.77897
0.48684 0.57331 0.64784 0.62026 0.68332 0.74828 0.80133
0.47113 0.56359 0.65668 0.63070 0.69646 0.76476 0.82043
0.45583 0.55429 0.66197 0.63355 0.70498 0.78154 0.83918
0.44038 0.54425 0.66915 0.64507 0.71777 0.80015 0.86174
0.41649 0.52712 0.66168 0.63515 0.71291 0.79901 0.85746
0.40308 0.51714 0.66537 0.64058 0.72298 0.81397 0.87416
0.38848 0.51017 0.67601 0.65126 0.73597 0.83320 0.90081
0.37773 0.50574 0.68510 0.65189 0.74626 0.85006 0.91904
0.36423 0.49636 0.69430 0.65769 0.76018 0.86779 0.93838
0.35391 0.49186 0.70091 0.66247 0.77013 0.88132 0.95675
0.34247 0.48474 0.71074 0.66635 0.77846 0.89593 0.97102
0.32997 0.47735 0.71558 0.66848 0.78676 0.91022 0.98809
0.31944 0.47026 0.72101 0.67408 0.79516 0.92141 1.00216
0.30936 0.46202 0.72297 0.67462 0.79835 0.92894 1.01056
0.29640 0.45293 0.72524 0.67485 0.80112 0.93492 1.01801
0.28652 0.44646 0.72277 0.67585 0.80317 0.94135 1.02595
0.27487 0.43761 0.72297 0.67210 0.80555 0.94424 1.02808
0.26540 0.42909 0.72470 0.67055 0.80435 0.94787 1.03447
0.25616 0.42166 0.72167 0.66549 0.80392 0.94544 1.03414
0.24730 0.41372 0.71943 0.66434 0.79995 0.94704 1.03295
0.23813 0.40461 0.71609 0.65657 0.79429 0.94230 1.03131
0.22904 0.39375 0.70581 0.64844 0.78694 0.93473 1.02373
0.21886 0.38249 0.69951 0.63895 0.77776 0.92556 1.01404
0.21003 0.37259 0.68696 0.62934 0.76604 0.91441 1.00078
0.20068 0.36383 0.67421 0.61736 0.75376 0.90158 0.98846
0.19218 0.35130 0.66236 0.60457 0.73948 0.88426 0.97171
0.18250 0.33889 0.64558 0.58848 0.72243 0.86517 0.94931
0.17484 0.32710 0.62958 0.57782 0.70567 0.84516 0.92889
0.16550 0.31251 0.61119 0.55906 0.68387 0.81998 0.90146
0.15841 0.30183 0.59232 0.53862 0.66210 0.79743 0.87702
0.15107 0.28921 0.57399 0.52137 0.64009 0.77187 0.84700
0.14169 0.27609 0.54906 0.49921 0.61470 0.74124 0.81616
0.13387 0.26362 0.52803 0.47991 0.59136 0.71397 0.78184
0.12603 0.25035 0.50352 0.45862 0.56428 0.68188 0.74824
0.12000 0.23969 0.48433 0.44028 0.54152 0.65496 0.72099
0.11161 0.22530 0.45594 0.41759 0.51333 0.62065 0.68228
0.10487 0.21234 0.43236 0.40213 0.48718 0.58956 0.64903
0.10112 0.20262 0.41008 0.37807 0.46406 0.56162 0.61732
0.09279 0.18949 0.38303 0.35498 0.43578 0.52691 0.58016
0.08718 0.17825 0.35947 0.33484 0.41015 0.49619 0.54643
0.08047 0.16543 0.33363 0.31249 0.38158 0.46231 0.50779
0.07555 0.15549 0.31241 0.29416 0.35786 0.43368 0.47632
0.07037 0.14358 0.28857 0.27274 0.33241 0.40177 0.44147
0.06493 0.13419 0.26695 0.25438 0.30853 0.37331 0.41054
0.06109 0.12503 0.24574 0.23614 0.28613 0.34553 0.37860
0.05636 0.11586 0.22592 0.21931 0.26375 0.31870 0.34948
0.05369 0.10553 0.20281 0.20014 0.23992 0.28955 0.31701
0.04838 0.09753 0.18463 0.18466 0.22164 0.26616 0.29121
0.04438 0.08849 0.16447 0.16923 0.20043 0.24193 0.26453
0.04103 0.08203 0.15075 0.15724 0.18479 0.22270 0.24287
0.03731 0.07494 0.13623 0.14346 0.16746 0.20179 0.22004
0.03422 0.06711 0.11940 0.13068 0.15084 0.18206 0.19767
0.03088 0.06179 0.10736 0.12033 0.13819 0.16623 0.17836
0.02834 0.05590 0.09384 0.10920 0.12405 0.14820 0.16052
0.02540 0.05038 0.08276 0.09952 0.11243 0.13404 0.14372
0.02362 0.04528 0.07262 0.09149 0.10050 0.12099 0.12928
0.02175 0.04107 0.06272 0.08335 0.09109 0.10879 0.11615
0.01943 0.03661 0.05319 0.07429 0.08047 0.09613 0.10194
0.01772 0.03320 0.04619 0.06866 0.07279 0.08654 0.09107
0.01670 0.03001 0.03793 0.06208 0.06514 0.07652 0.08061
0.01437 0.02882 0.03084 0.05702 0.05773 0.06860 0.07183
0.01317 0.02491 0.02602 0.05338 0.05032 0.06020 0.06246
0.01286 0.02337 0.02248 0.04811 0.04673 0.05524 0.05603
0.01152 0.02051 0.01609 0.04342 0.04028 0.04776 0.04845
0.01070 0.01856 0.01162 0.03954 0.03565 0.04263 0.04168
0.01026 0.01727 0.00843 0.03571 0.03170 0.03636 0.03677
0.00923 0.01558 0.00436 0.03265 0.02801 0.03254 0.03108
0.00867 0.01406 0.00147 0.03036 0.02495 0.02928 0.02710
0.00754 0.01188 -0.00191 0.02812 0.02155 0.02519 0.02322
0.00769 0.01271 -0.00272 0.02678 0.01978 0.02329 0.01811
0.00623 0.01096 -0.00630 0.02475 0.01784 0.01995 0.01733
0.00600 0.01002 -0.00738 0.02274 0.01574 0.01805 0.01262
0.00534 0.00968 -0.00812 0.02245 0.01427 0.01536 0.01334
0.00503 0.00816 -0.01059 0.02069 0.01230 0.01403 0.01132
0.00581 0.00824 -0.01146 0.01936 0.01132 0.01262 0.00950
0.00518 0.00770 -0.01277 0.01897 0.01002 0.01118 0.00773
0.00486 0.00732 -0.01351 0.01757 0.00922 0.01005 0.00689
0.00460 0.00674 -0.01398 0.01683 0.00758 0.00824 0.00436
0.00454 0.00698 -0.01440 0.01710 0.00789 0.00763 0.00495
0.00414 0.00616 -0.01551 0.01624 0.00634 0.00707 0.00376
0.00397 0.00592 -0.01605 0.01516 0.00596 0.00645 0.00238
0.00430 0.00579 -0.01605 0.01562 0.00573 0.00619 0.00212
0.00430 0.00579 -0.01605 0.01562 0.00573 0.00619 0.00212

6.97 7.59 8.22 8.75 9.04 9.57 10.51

1.01726 0.96636 0.67167 0.45192 0.55733 0.61248 0.79565
1.15863 1.05368 0.91361 0.60264 0.66696 0.79723 0.88924
1.19316 1.06962 0.99153 0.75566 0.82277 0.89445 0.94521
1.17861 1.08927 1.01193 0.81566 0.89363 0.93058 0.99374
1.14346 1.07175 1.03543 0.85168 0.93316 0.96587 1.02614
1.09169 1.04896 1.03469 0.87325 0.95227 0.98116 1.0583
1.02495 1.00386 1.01986 0.87447 0.95098 0.98244 1.07393
0.97551 0.9721 0.99939 0.86562 0.93882 0.96983 1.05467
0.88555 0.91188 0.95637 0.83557 0.91387 0.95606 1.01205
0.8384 0.87428 0.92787 0.82036 0.8899 0.94225 0.99698
0.78055 0.82855 0.89414 0.81575 0.86227 0.91629 0.95967
0.70955 0.76479 0.84625 0.75676 0.81177 0.87758 0.90933
0.67546 0.73398 0.806 0.72745 0.77664 0.8443 0.88386
0.63229 0.68932 0.74902 0.68815 0.72909 0.79527 0.83967
0.60311 0.65359 0.70247 0.65179 0.6844 0.74608 0.79252
0.58842 0.63355 0.66691 0.62419 0.65102 0.70528 0.75399
0.58532 0.62545 0.65069 0.61235 0.63659 0.67217 0.73228
0.59007 0.61727 0.62644 0.58467 0.60261 0.62271 0.67858
0.59936 0.61702 0.60905 0.56508 0.57861 0.58103 0.63288
0.60409 0.62104 0.60042 0.55113 0.55189 0.54598 0.59826
0.62132 0.62887 0.58774 0.53504 0.52725 0.50659 0.55625
0.64053 0.63686 0.58243 0.52256 0.50862 0.47561 0.52433
0.65926 0.64518 0.57353 0.51466 0.49954 0.45199 0.49759
0.67876 0.65078 0.56162 0.50282 0.47857 0.43077 0.46983
0.69643 0.65879 0.5545 0.49204 0.46474 0.41354 0.44578
0.70804 0.67006 0.55418 0.49159 0.45787 0.40865 0.4297
0.71974 0.67641 0.54717 0.48737 0.4518 0.40373 0.41649
0.73451 0.68321 0.54626 0.48542 0.44953 0.39744 0.40955
0.74385 0.68865 0.54479 0.48693 0.44953 0.40003 0.40674
0.75316 0.69168 0.5435 0.50664 0.45235 0.4048 0.40753
0.76253 0.69827 0.56172 0.51063 0.45815 0.41149 0.41275
0.7699 0.70295 0.55036 0.50041 0.46474 0.42077 0.4202
0.77739 0.70349 0.55425 0.50469 0.47183 0.42916 0.42969
0.77659 0.7071 0.55966 0.51223 0.4803 0.44185 0.44209
0.77384 0.7091 0.56251 0.51923 0.48684 0.44946 0.45281
0.77058 0.7092 0.57081 0.52666 0.49716 0.45453 0.46511
0.76727 0.70699 0.57502 0.52959 0.50649 0.46581 0.47908
0.75935 0.70684 0.58231 0.53695 0.51657 0.48231 0.49491
0.75069 0.70609 0.58605 0.55002 0.52405 0.49343 0.50912
0.74035 0.70337 0.59173 0.54879 0.53272 0.50568 0.52418
0.72763 0.6995 0.59821 0.55457 0.54233 0.52143 0.54138
0.7189 0.69202 0.60219 0.55906 0.55053 0.53383 0.55422
0.70116 0.68615 0.6055 0.56327 0.55819 0.54765 0.57194
0.68384 0.67718 0.61133 0.56658 0.56913 0.5646 0.59162
0.66862 0.66935 0.61396 0.5702 0.57692 0.57804 0.60769
0.64987 0.65836 0.61716 0.57289 0.58604 0.59193 0.62333
0.62934 0.64832 0.62022 0.5776 0.59573 0.60944 0.64404
0.6112 0.63847 0.62435 0.58055 0.60447 0.62395 0.66158
0.59121 0.62673 0.63082 0.58356 0.61522 0.64083 0.67736
0.57335 0.61617 0.63341 0.5866 0.62305 0.65453 0.69598
0.55684 0.60829 0.64031 0.59214 0.63479 0.67321 0.71476
0.53585 0.59605 0.64719 0.59575 0.64579 0.69124 0.73968
0.51805 0.58536 0.65133 0.59554 0.65791 0.70652 0.75772
0.50134 0.57629 0.65535 0.59887 0.66803 0.72345 0.77685
0.48254 0.56752 0.66389 0.60464 0.67759 0.74209 0.79921
0.46683 0.5578 0.67273 0.61508 0.69073 0.75857 0.81831
0.45153 0.5485 0.67802 0.61793 0.69925 0.77535 0.83706
0.43608 0.53846 0.6852 0.62945 0.71204 0.79396 0.85962
0.41219 0.52133 0.67773 0.61953 0.70718 0.79282 0.85534
0.39878 0.51135 0.68142 0.62496 0.71725 0.80778 0.87204
0.38418 0.50438 0.69206 0.63564 0.73024 0.82701 0.89869
0.37343 0.49995 0.70115 0.63627 0.74053 0.84387 0.91692
0.35993 0.49057 0.71035 0.64207 0.75445 0.8616 0.93626
0.34961 0.48607 0.71696 0.64685 0.7644 0.87513 0.95463
0.33817 0.47895 0.72679 0.65073 0.77273 0.88974 0.9689
0.32567 0.47156 0.73163 0.65286 0.78103 0.90403 0.98597
0.31514 0.46447 0.73706 0.65846 0.78943 0.91522 1.00004
0.30506 0.45623 0.73902 0.659 0.79262 0.92275 1.00844
0.2921 0.44714 0.74129 0.65923 0.79539 0.92873 1.01589
0.28222 0.44067 0.73882 0.66023 0.79744 0.93516 1.02383
0.27057 0.43182 0.73902 0.65648 0.79982 0.93805 1.02596
0.2611 0.4233 0.74075 0.65493 0.79862 0.94168 1.03235
0.25186 0.41587 0.73772 0.64987 0.79819 0.93925 1.03202
0.243 0.40793 0.73548 0.64872 0.79422 0.94085 1.03083
0.23383 0.39882 0.73214 0.64095 0.78856 0.93611 1.02919
0.22474 0.38796 0.72186 0.63282 0.78121 0.92854 1.02161
0.21456 0.3767 0.71556 0.62333 0.77203 0.91937 1.01192
0.20573 0.3668 0.70301 0.61372 0.76031 0.90822 0.99866
0.19638 0.35804 0.69026 0.60174 0.74803 0.89539 0.98634
0.18788 0.34551 0.67841 0.58895 0.73375 0.87807 0.96959
0.1782 0.3331 0.66163 0.57286 0.7167 0.85898 0.94719
0.17054 0.32131 0.64563 0.5622 0.69994 0.83897 0.92677
0.1612 0.30672 0.62724 0.54344 0.67814 0.81379 0.89934
0.15411 0.29604 0.60837 0.523 0.65637 0.79124 0.8749
0.14677 0.28342 0.59004 0.50575 0.63436 0.76568 0.84488
0.13739 0.2703 0.56511 0.48359 0.60897 0.73505 0.81404
0.12957 0.25783 0.54408 0.46429 0.58563 0.70778 0.77972
 0.12173 0.24456 0.51957 0.443 0.55855 0.67569 0.74612
0.1157 0.2339 0.50038 0.42466 0.53579 0.64877 0.71887
0.10731 0.21951 0.47199 0.40197 0.5076 0.61446 0.68016
0.10057 0.20655 0.44841 0.38651 0.48145 0.58337 0.64691
0.09682 0.19683 0.42613 0.36245 0.45833 0.55543 0.6152
0.08849 0.1837 0.39908 0.33936 0.43005 0.52072 0.57804
0.08288 0.17246 0.37552 0.31922 0.40442 0.49 0.54431
0.07617 0.15964 0.34968 0.29687 0.37585 0.45612 0.50567
0.07125 0.1497 0.32846 0.27854 0.35213 0.42749 0.4742
0.06607 0.13779 0.30462 0.25712 0.32668 0.39558 0.43935
0.06063 0.1284 0.283 0.23876 0.3028 0.36712 0.40842
0.05679 0.11924 0.26179 0.22052 0.2804 0.33934 0.37648
0.05206 0.11007 0.24197 0.20369 0.25802 0.31251 0.34736
0.04939 0.09974 0.21886 0.18452 0.23419 0.28336 0.31489
0.04408 0.09174 0.20068 0.16904 0.21591 0.25997 0.28909
0.04008 0.0827 0.18052 0.15361 0.1947 0.23574 0.26241
0.03673 0.07624 0.1668 0.14162 0.17906 0.21651 0.24075
0.03301 0.06915 0.15228 0.12784 0.16173 0.1956 0.21792
0.02992 0.06132 0.13545 0.11506 0.14511 0.17587 0.19555
0.02658 0.056 0.12341 0.10471 0.13246 0.16004 0.17624
0.02404 0.05011 0.10989 0.09358 0.11832 0.14201 0.1584
0.0211 0.04459 0.09881 0.0839 0.1067 0.12785 0.1416
0.01932 0.03949 0.08867 0.07587 0.09477 0.1148 0.12716
0.01745 0.03528 0.07877 0.06773 0.08536 0.1026 0.11403
0.01513 0.03082 0.06924 0.05867 0.07474 0.08994 0.09982
0.01342 0.02741 0.06224 0.05304 0.06706 0.08035 0.08895
0.0124 0.02422 0.05398 0.04646 0.05941 0.07033 0.07849
0.01007 0.02303 0.04689 0.0414 0.052 0.06241 0.06971
0.00887 0.01912 0.04207 0.03776 0.04459 0.05401 0.06034
0.00856 0.01758 0.03853 0.03249 0.041 0.04905 0.05391
0.00722 0.01472 0.03214 0.0278 0.03455 0.04157 0.04633
0.0064 0.01277 0.02767 0.02392 0.02992 0.03644 0.03956
0.00596 0.01148 0.02448 0.02009 0.02597 0.03017 0.03465
0.00493 0.00979 0.02041 0.01703 0.02228 0.02635 0.02896
0.00437 0.00827 0.01752 0.01474 0.01922 0.02309 0.02498
0.00324 0.00609 0.01414 0.0125 0.01582 0.019 0.0211
0.00339 0.00692 0.01333 0.01116 0.01405 0.0171 0.01599
0.00193 0.00517 0.00975 0.00913 0.01211 0.01376 0.01521
0.0017 0.00423 0.00867 0.00712 0.01001 0.01186 0.0105
0.00104 0.00389 0.00793 0.00683 0.00854 0.00917 0.01122
0.00073 0.00237 0.00546 0.00507 0.00657 0.00784 0.0092
0.00151 0.00245 0.00459 0.00374 0.00559 0.00643 0.00738
0.00088 0.00191 0.00328 0.00335 0.00429 0.00499 0.00561
0.00056 0.00153 0.00254 0.00195 0.00349 0.00386 0.00477
0.0003 0.00095 0.00207 0.00121 0.00185 0.00205 0.00224
0.00024 0.00119 0.00165 0.00148 0.00216 0.00144 0.00283
-0.00016 0.00037 0.00054 0.00062 0.00061 0.00088 0.00164
-0.00033 0.00013 0 -0.00046 0.00023 0.00026 0.00026
 0 0 0 0 0 0 0

etermined graphically are λmax = 380 nm and λmin = 290 nm)

6.97 7.59 8.22 8.75 9.04 9.57 10.51
0.19363 0.14273 -0.15196 -0.37171 -0.2663 -0.21115 -0.02798
0.03715 -0.0678 -0.20787 -0.51884 -0.45452 -0.32425 -0.23224
0.03447 -0.08907 -0.16716 -0.40303 -0.33592 -0.26424 -0.21348
0.02922 -0.06012 -0.13746 -0.33373 -0.25576 -0.21881 -0.15565
0.01646 -0.05525 -0.09157 -0.27532 -0.19384 -0.16113 -0.10086
0.0199 -0.02283 -0.0371 -0.19854 -0.11952 -0.09063 -0.01349
0.01779 -0.0033 0.0127 -0.13269 -0.05618 -0.02472 0.06677
0.02731 0.0239 0.05119 -0.08258 -0.00938 0.02163 0.10647
0.02956 0.05589 0.10038 -0.02042 0.05788 0.10007 0.15606
0.03218 0.06806 0.12165 0.01414 0.08368 0.13603 0.19076
0.02919 0.07719 0.14278 0.06439 0.11091 0.16493 0.20831
0.03599 0.09123 0.17269 0.0832 0.13821 0.20402 0.23577
0.03406 0.09258 0.1646 0.08605 0.13524 0.2029 0.24246
0.03049 0.08752 0.14722 0.08635 0.12729 0.19347 0.23787
0.0274 0.07788 0.12676 0.07608 0.10869 0.17037 0.21681
0.01759 0.06272 0.09608 0.05336 0.08019 0.13445 0.18316
0.01149 0.05162 0.07686 0.03852 0.06276 0.09834 0.15845
0.00023 0.02743 0.0366 -0.00517 0.01277 0.03287 0.08874
-0.01776 -0.0001 -0.00807 -0.05204 -0.03851 -0.03609 0.01576
-0.03751 -0.02056 -0.04118 -0.09047 -0.08971 -0.09562 -0.04334
-0.05061 -0.04306 -0.08419 -0.13689 -0.14468 -0.16534 -0.11568
-0.05846 -0.06213 -0.11656 -0.17643 -0.19037 -0.22338 -0.17466
-0.06158 -0.07566 -0.14731 -0.20618 -0.2213 -0.26885 -0.22325
-0.06635 -0.09433 -0.18349 -0.24229 -0.26654 -0.31434 -0.27528
-0.069 -0.10664 -0.21093 -0.27339 -0.30069 -0.35189 -0.31965
-0.08027 -0.11825 -0.23413 -0.29672 -0.33044 -0.37966 -0.35861
-0.08185 -0.12518 -0.25442 -0.31422 -0.34979 -0.39786 -0.3851
-0.07894 -0.13024 -0.26719 -0.32803 -0.36392 -0.41601 -0.4039
-0.07932 -0.13452 -0.27838 -0.33624 -0.37364 -0.42314 -0.41643
-0.07316 -0.13464 -0.28282 -0.31968 -0.37397 -0.42152 -0.41879
-0.06683 -0.13109 -0.26764 -0.31873 -0.37121 -0.41787 -0.41661
-0.07134 -0.13829 -0.29088 -0.34083 -0.3765 -0.42047 -0.42104
-0.05049 -0.12439 -0.27363 -0.32319 -0.35605 -0.39872 -0.39819
-0.04791 -0.1174 -0.26484 -0.31227 -0.3442 -0.38265 -0.38241
-0.04627 -0.11101 -0.2576 -0.30088 -0.33327 -0.37065 -0.3673
-0.04263 -0.10401 -0.2424 -0.28655 -0.31605 -0.35868 -0.3481
-0.0365 -0.09678 -0.22875 -0.27418 -0.29728 -0.33796 -0.32469
-0.03221 -0.08472 -0.20925 -0.25461 -0.27499 -0.30925 -0.29665
-0.0291 -0.0737 -0.19374 -0.22977 -0.25574 -0.28636 -0.27067
-0.02427 -0.06125 -0.17289 -0.21583 -0.2319 -0.25894 -0.24044
-0.01954 -0.04767 -0.14896 -0.1926 -0.20484 -0.22574 -0.20579
-0.01088 -0.03776 -0.12759 -0.17072 -0.17925 -0.19595 -0.17556
-0.00766 -0.02267 -0.10332 -0.14555 -0.15063 -0.16117 -0.13688
-0.00133 -0.00799 -0.07384 -0.11859 -0.11604 -0.12057 -0.09355
0.00579 0.00652 -0.04887 -0.09263 -0.08591 -0.08479 -0.05514
0.00991 0.0184 -0.0228 -0.06707 -0.05392 -0.04803 -0.01663
0.01645 0.03543 0.00733 -0.03529 -0.01716 -0.00345 0.03115
0.02218 0.04945 0.03533 -0.00847 0.01545 0.03493 0.07256
0.02745 0.06297 0.06706 0.0198 0.05146 0.07707 0.1136
0.03207 0.07489 0.09213 0.04532 0.08177 0.11325 0.1547
0.03668 0.08813 0.12015 0.07198 0.11463 0.15305 0.1946
0.04188 0.10208 0.15322 0.10178 0.15182 0.19727 0.24571
0.0459 0.11321 0.17918 0.12339 0.18576 0.23437 0.28557
0.0512 0.12615 0.20521 0.14873 0.21789 0.27331 0.32671
0.05548 0.14046 0.23683 0.17758 0.25053 0.31503 0.37215
0.06073 0.1517 0.26663 0.20898 0.28463 0.35247 0.41221
0.06582 0.16279 0.29231 0.23222 0.31354 0.38964 0.45135
0.07234 0.17472 0.32146 0.26571 0.3483 0.43022 0.49588
0.07637 0.18551 0.34191 0.28371 0.37136 0.457 0.51952
0.08075 0.19332 0.36339 0.30693 0.39922 0.48975 0.55401
0.08353 0.20373 0.39141 0.33499 0.42959 0.52636 0.59804
0.08934 0.21586 0.41706 0.35218 0.45644 0.55978 0.63283
0.09583 0.22647 0.44625 0.37797 0.49035 0.5975 0.67216
0.10017 0.23663 0.46752 0.39741 0.51496 0.62569 0.70519
0.10361 0.24439 0.49223 0.41617 0.53817 0.65518 0.73434
0.10863 0.25452 0.51459 0.43582 0.56399 0.68699 0.76893
0.11178 0.26111 0.5337 0.4551 0.58607 0.71186 0.79668
0.11602 0.26719 0.54998 0.46996 0.60358 0.73371 0.8194
0.119 0.27404 0.56819 0.48613 0.62229 0.75563 0.84279
0.12129 0.27974 0.57789 0.4993 0.63651 0.77423 0.8629
0.12379 0.28504 0.59224 0.5097 0.65304 0.79127 0.87918
0.12368 0.28588 0.60333 0.51751 0.6612 0.80426 0.89493
0.12802 0.29203 0.61388 0.52603 0.67435 0.81541 0.90818
0.12912 0.29405 0.6216 0.53484 0.68034 0.82697 0.91695
0.12923 0.29422 0.62754 0.53635 0.68396 0.83151 0.92459
0.12959 0.29281 0.62671 0.53767 0.68606 0.83339 0.92646
0.12974 0.29188 0.63074 0.53851 0.68721 0.83455 0.9271
0.1279 0.28897 0.62518 0.53589 0.68248 0.83039 0.92083
0.11835 0.28001 0.61223 0.52371 0.67 0.81736 0.90831
0.12551 0.28314 0.61604 0.52658 0.67138 0.8157 0.90722
0.12441 0.27931 0.60784 0.51907 0.66291 0.80519 0.8934
 0.12166 0.27243 0.59675 0.51332 0.65106 0.79009 0.87789
0.11957 0.26509 0.58561 0.50181 0.63651 0.77216 0.85771
0.11323 0.25516 0.56749 0.48212 0.61549 0.75036 0.83402
0.11448 0.25113 0.55775 0.47346 0.60207 0.73339 0.81259
0.11036 0.24327 0.53808 0.45656 0.58194 0.70802 0.78701
0.10658 0.23484 0.52109 0.4413 0.56264 0.68479 0.75673
0.10257 0.2254 0.50041 0.42384 0.53939 0.65653 0.72696
0.09956 0.21776 0.48424 0.40852 0.51965 0.63263 0.70273
0.09374 0.20594 0.45842 0.3884 0.49403 0.60089 0.66659
0.07398 0.17996 0.42182 0.35992 0.45486 0.55678 0.62032
0.087 0.18701 0.41631 0.35263 0.44851 0.54561 0.60538
0.07949 0.1747 0.39008 0.33036 0.42105 0.51172 0.56904
0.07651 0.16609 0.36915 0.31285 0.39805 0.48363 0.53794
0.0712 0.15467 0.34471 0.2919 0.37088 0.45115 0.5007
0.0667 0.14515 0.32391 0.27399 0.34758 0.42294 0.46965
0.06228 0.134 0.30083 0.25333 0.32289 0.39179 0.43556
0.05756 0.12533 0.27993 0.23569 0.29973 0.36405 0.40535
0.05261 0.11506 0.25761 0.21634 0.27622 0.33516 0.3723
0.04846 0.10647 0.23837 0.20009 0.25442 0.30891 0.34376
0.04686 0.09721 0.21633 0.18199 0.23166 0.28083 0.31236
0.04217 0.08983 0.19877 0.16713 0.214 0.25806 0.28718
0.03869 0.08131 0.17913 0.15222 0.19331 0.23435 0.26102
0.03559 0.0751 0.16566 0.14048 0.17792 0.21537 0.23961
0.03197 0.06811 0.15124 0.1268 0.16069 0.19456 0.21688
0.02913 0.06053 0.13466 0.11427 0.14432 0.17508 0.19476
0.02646 0.05588 0.12329 0.10459 0.13234 0.15992 0.17612
0.02339 0.04946 0.10924 0.09293 0.11767 0.14136 0.15775
0.02045 0.04394 0.09816 0.08325 0.10605 0.1272 0.14095
0.01888 0.03905 0.08823 0.07543 0.09433 0.11436 0.12672
0.01722 0.03505 0.07854 0.0675 0.08513 0.10237 0.1138
0.01451 0.0302 0.06862 0.05805 0.07412 0.08932 0.0992
0.01362 0.02761 0.06244 0.05324 0.06726 0.08055 0.08915
0.01232 0.02414 0.0539 0.04638 0.05933 0.07025 0.07841
0.01024 0.0232 0.04706 0.04157 0.05217 0.06258 0.06988
0.00925 0.0195 0.04245 0.03814 0.04497 0.05439 0.06072
0.00847 0.01749 0.03844 0.0324 0.04091 0.04896 0.05382
0.00781 0.01531 0.03273 0.02839 0.03514 0.04216 0.04692
0.00662 0.01299 0.02789 0.02414 0.03014 0.03666 0.03978
0.00624 0.01176 0.02476 0.02037 0.02625 0.03045 0.03493
0.0059 0.01076 0.02138 0.018 0.02325 0.02732 0.02993
0.00497 0.00887 0.01812 0.01534 0.01982 0.02369 0.02558
0.00455 0.0074 0.01545 0.01381 0.01713 0.02031 0.02241
0.00361 0.00714 0.01355 0.01138 0.01427 0.01732 0.01621
0.00235 0.00559 0.01017 0.00955 0.01253 0.01418 0.01563
-0.01328 -0.01075 -0.00631 -0.00786 -0.00497 -0.00312 -0.00448
0.00124 0.00409 0.00813 0.00703 0.00874 0.00937 0.01142
0.00056 0.0022 0.00529 0.0049 0.0064 0.00767 0.00903
-0.00252 -0.00158 0.00056 -0.00029 0.00156 0.0024 0.00335
 -0.00074 0.00029 0.00166 0.00173 0.00267 0.00337 0.00399
0.0007 0.00167 0.00268 0.00209 0.00363 0.004 0.00491
0.00074 0.00139 0.00251 0.00165 0.00229 0.00249 0.00268
-0.01491 -0.01396 -0.0135 -0.01367 -0.01299 -0.01371 -0.01232
-0.00842 -0.00789 -0.00772 -0.00764 -0.00765 -0.00738 -0.00662
0.00161 0.00207 0.00194 0.00148 0.00217 0.0022 0.0022

0 0 0 0 0 0 0

ine acetate (13a)

al wavelengths)
A b s o rb a n c e d iffe re n c e

Absolute(λmin) Sum = total absorbance

0 0
0.01028 0.01645
0.06403 0.08735
0.03735 0.10623
Total absorbance difference vs p
0.06683 0.19642 pKa Determination
0.13109 0.4239
0.26764 0.89435 2
0.31873 0.8564
0.37121 1.05727
0.41787 1.25126
0.41661 1.34307 1
0.46358 1.33006

0
2 3 4 5 6 7 8 9 10
pH
 12.24
0.02005
0.54804
0.73220
0.87257
0.90028
0.92045
0.93171
0.91944
0.90777
0.89915 Raw data
0.87221
0.84313 1.20000
0.82001
0.77882
0.73648
1.00000
0.70087
0.67688
Absorbance

0.62408
0.58634 0.80000
0.55379
0.51553
0.48058 0.60000
0.45434
0.42759
0.40851 0.40000
0.39424
0.38256
0.37382
0.20000
0.36385
0.36362
0.37699
0.38266 0.00000
0.39032 250 300 350 400
0.39509
0.41375 -0.20000 Wavelength (nm)
0.41642
0.42843
0.44643
0.46592
0.47592
0.49739
0.51120
0.52641
0.54409
0.56041
0.57607
0.59504
0.61150
0.62258
0.64681
0.66608
0.68668
0.70610
0.71889
0.74601
0.76277
0.78224
0.80430
0.80424
0.82204
0.83790
0.86291
0.88059
0.89748
0.91317
0.92716
0.94483
0.95277
0.96317
0.96901
0.97488
0.97955
0.97955
0.98244
0.97967
0.97284
0.96572
0.95424
0.94085
0.92372
0.90619
0.88704
0.86307
0.83924
0.81065
0.78295
0.75606
0.72280
0.69521
0.66063
0.62867
0.59964
0.56379
0.53240
0.49623
0.46648
0.43362
0.40395
0.37369
0.34569
0.31399
0.29044
0.26487
0.24420
0.22215
0.20085
0.18112
0.16562
0.14999
0.13563
0.12268
0.10929
0.09708
0.08884
0.07969
0.06997
0.06504
0.05699
0.05142
0.04522
0.04061
0.03692
0.03172
0.03011
0.02741
0.02426
0.02154
0.02020
0.01921
0.01713
0.01442
0.01410
0.01401
0.01136
0.01130
0.01121
0.01121

12.24
0.00884
0.53683
0.72099
0.86136
0.88907
0.90924
0.9205
0.90823 Normalized
0.89656
0.88794 1.2
0.861
0.83192
0.8088 1
0.76761
Absorbance

0.72527
0.68966
0.66567 0.8
0.61287
0.57513
0.54258 0.6
0.50432
0.46937
0.44313
0.4
0.41638
0.3973
0.38303
0.37135 0.2
0.36261
0.35264
0.35241
0
0.36578 250 300 350
0.37145
0.37911
0.38388 -0.2 Wavelen
0.40254
0.40521
0.41722
0.43522
0.45471
0.46471
0.48618
0.49999
0.5152
0.53288
0.5492
0.56486
0.58383
0.60029
0.61137
0.6356
0.65487
0.67547
0.69489
0.70768
0.7348
0.75156
0.77103
0.79309
0.79303
0.81083
0.82669
0.8517
0.86938
0.88627
0.90196
0.91595
0.93362
0.94156
0.95196
0.9578
0.96367
0.96834
0.96834
0.97123
0.96846
0.96163
0.95451
0.94303
0.92964
0.91251
0.89498
0.87583
0.85186
0.82803
0.79944
0.77174
0.74485
0.71159
0.684
0.64942
0.61746
0.58843
0.55258
0.52119
0.48502
0.45527
0.42241
0.39274
0.36248
0.33448
0.30278
0.27923
0.25366
0.23299
0.21094
0.18964
0.16991
0.15441
0.13878
0.12442
0.11147
0.09808
0.08587
0.07763
0.06848
0.05876
0.05383
0.04578
0.04021
0.03401
0.0294
0.02571
0.02051
0.0189
0.0162
0.01305
0.01033
0.00899
0.008
0.00592
0.00321
0.00289
0.0028
0.00015
0.00009
 0

12.24
-0.81479
-0.58465
-0.4377
-0.28803
-0.23793
-0.16255
-0.08666
-0.03997
0.04057
0.08172
0.10964
0.15836
0.1674
0.16581 Spectral difference
0.14956
0.11883
0.09184 1
0.02303
-0.04199
-0.09902
-0.16761
-0.22962
-0.27771
Absorbance

-0.32873 0.5
-0.36813
-0.40528
-0.43024
-0.45084
-0.47053
-0.47391
-0.46358 0
-0.46979
-0.44877
 0

-0.44062
-0.41757
-0.408
-0.38655
-0.35634 -0.5
-0.32508
-0.29991
-0.26099
-0.22979
-0.19362
-0.15229
-0.11363 -1
-0.0751
250 270 290 310 330 350 370 390
-0.02906 Wavelength (nm)
0.01127
318 318 nm
0.04761
0.09432
0.13471
0.1815
0.22274
0.25754
0.30774
0.34546
0.38532
0.42935
0.45721
0.4928
0.52604
0.56761
0.60528
0.63683
0.6674
0.69891
0.73026
0.75252
0.77886
0.79687
0.81689
0.83092
0.8445
0.85735
0.86386
0.86648
0.86969
0.8652
0.85161
0.85014
0.84119
 0.82695
0.81023
0.78715
0.76715
0.74471
0.72186
0.69243
0.66786
0.63585
0.59087
0.57861
0.54358
0.51482
0.48005
0.45072
0.41862
0.38967
0.3583
0.33088
0.30025
0.27732
0.25227
0.23185
0.2099
0.18885
0.16979
0.15376
0.13813
0.12398
0.11124
0.09746
0.08607
0.07755
0.06865
0.05914
0.05374
0.04637
0.04043
0.03429
0.03037
0.02631
0.02182
0.01912
0.01662
-0.00193
0.01053
0.00882
0.00397
 0.0043
0.00335
0.00333
-0.01235
-0.00811
0.00203

rbance difference vs pH
Ka Determination

6 7 8 9 10 11 12 13
pH
 Raw data

350 400 450 500

Wavelength (nm)
Normalized data

350 400 450 500

Wavelength (nm)
Spectral difference plot

Column
B
Column
C
Column
D
Column
E
Column
F
Column
G
Column
H
Column
I
Column
J
Column
K
Column
L
Column
 G
Column
H
Column
I
Column
J
Column
K
Column
L
Column
M

350 370 390 410 430 450 470 490

Wavelength (nm)
318 nm 400
500
Column
B
Column
C
Column
D
Column
E
Column
F
Column
G
Column
H
Column
I
Column
J
Column
K
Column
L
Column
G
Column
H
Column
I
Column
J
Column
K
Column
L
Column
M