12/26/2018 GENETICALLY MODIFIED (GM) CROP: PROBLEM AND PROSPECT What is GM (Genetically Moditied) CROP? Developing crop of conferring or deleting 8 specific target trat to the host crop by the insertion of defined DNA sequences (trom any souree). Whatarethe alt ‘approach? 06 with elassieal Breeding Alorovacterurn the sallbom gram negative Bacteria Jcrowmcaut. ‘Causal agent Acrotacnvom neta Useful for development of oH crops. Aagrobacterium...general features] +Gram-negative sNon-sporing +Rod-shaped ‘Closely related to Rhizobium How a GH crops developed? Insertion of desired ONA and marker gone into 3 particular region (-ONA border ofa sal-bome Agrobacterium called engineered Agrobacterium Infection of plant (host by engineered ‘Rgrobacterim Growing of complete pant from infected leaves Intoaprestandaraed Tissue culture media, ‘Screening hardening and testing of desired plant Feld evaluation with sit monitoringPlant Transformation * Gene of Interest dentifiction, cloning and sequencing) + Standardastion of regenartion media and hormone forte desired host pant + Insertion of gene of intrest into the FONA, border Agrobactertum (aso tome actor. * Cocuttivation of Agrobacterium culture and plant par (expan) + Regnerationof plant onthe pre-standardieed regeneration media * Selection of plant with gene of intrest! + Fle testing for checking of desirabie performance BENEFITS TO THE SOCIETY * Increased productivity of food ane ted crops ‘Decreased human heath hazards ‘Reuces polation of the environment stnereased nutetive value with preter ap precision “Value action for users end users (consumers) 12/26/201812/26/2018 Currently approved genetically modiid pants have ‘bean developed to express one or more a the following basic phenetypie charactorsties a) Fatty ack composition b)Amino acid composition ©) Forty restoration Herc oierance {e)insecttolrance 1 Malo story ‘9)Meaied colours ‘n)Niotine eution 4) Delayed ripening 1) Virus resistance ISSUES FREQUENTLY RAISED AGAINST GM (CROPSIFOODS, ‘Te potential for gone transfer from genetically ‘modifies plants to gut micro loa and mammatian col, ‘The safety of aniiotle resistance gene as marker Potential allergenicity of foods ‘oe puna dears hel crags tn ‘Paredh tay best ae ese ast