Initial conditios

Flow rate 2,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 100 %Acetonitrile 0

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 17,57 8,50

Acetophenone 21,79 10,53

Propiophenone 56,51 27,32

Ketoprofen 134,92 65,24

benzophenone 237,54 114,86

Flow rate 2,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 75 %Acetonitrile 25
Compound name: tR(Retention time, σ total (s)
min)
3-nitrophenol 2,45 1,17

Acetophenone 2,89 1,39

Propiophenone 6,66 3,19

Ketoprofen 9,07 4,35

benzophenone 21,30 10,21

Flow rate 2,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 50 %Acetonitrile 50

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 0,74 1,17

Acetophenone 0,79 1,39

Ketoprofen 1,07 3,19

Propiophenone 1,20 4,35

benzophenone 2,35 10,21

Flow rate 6,0 Injection 3,0 Column 100
(ml/min) Volume(μL) Lenght
% Water 100 %Acetonitrile 0

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 5,43 3,39

Acetophenone 6,95 4,33

Propiophenone 18,06 11,24

Ketoprofen 42,55 26,49

benzophenone 75,37 46,93

Flow rate 6,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 75 %Acetonitrile 25

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 0,78 0,50

Acetophenone 0,93 0,59

Propiophenone 2,14 1,31

Ketoprofen 2,89 1,77

benzophenone 6,78 4,13

Flow rate 6,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 50 %Acetonitrile 50

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 0,24 0,22

Acetophenone 0,26 0,22

Propiophenone 0,39 0,28

Ketoprofen 0,35 0,26

benzophenone 0,75 0,46

Flow rate 2,0 Injection 6,0 Column 100
(ml/min) Volume(μL) Lenght
% Water 100 %Acetonitrile 0

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 16,31 7,82

Acetophenone 20,87 10,01

Propiophenone 54,18 25,99

Ketoprofen 127,65 61,24

benzophenone 226,12 108,48

Flow rate 2,0 Injection 6,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 75 %Acetonitrile 25
Compound name: tR(Retention time, σ total (s)
min)
3-nitrophenol 2,34 1,15

Acetophenone 2,81 3,07

Propiophenone 6,43 3,07

Ketoprofen 8,68 4,14

benzophenone 20,35 9,69

Flow rate 2,0 Injection 6,0 Column 100

(ml/min) Volume(μL) Lenght
% Water 50 %Acetonitrile 50

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 0,74 0,43

Acetophenone 0,79 0,45

Propiophenone 1,18 0,61

Ketoprofen 1,05 0,55

benzophenone 2,27 1,09

Flow rate 2,0 Injection 3,0 Column 200
(ml/min) Volume(μL) Lenght
% Water 100 %Acetonitrile 0

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 35,16 12,02

Acetophenone 43,59 14,90

Propiophenone 113,04 38,65

Ketoprofen 269,85 92,26

benzophenone 475,08 162,44

Flow rate 2,0 Injection 3,0 Column 200

(ml/min) Volume(μL) Lenght
% Water 75 %Acetonitrile 25

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 4,90 1,66

Acetophenone 5,79 1,96

Propiophenone 13,33 4,52

Ketoprofen 18,15 6,16

benzophenone 42,61 14,44

Flow rate 2,0 Injection 3,0 Column 200

(ml/min) Volume(μL) Lenght
% Water 50 %Acetonitrile 50

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 1,49 0,51

Acetophenone 1,59 0,53

Propiophenone 2,41 0,81

Ketoprofen 2,15 0,72

benzophenone 4,70 1,56

Flow rate 2,0 Injection 3,0 Column 100
(ml/min) Volume(μL) Lenght
Time 10 %B 50

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 4,96 1,25

Propiophenone 7,58 1,37

benzophenone 10,25 1,32

Flow rate 2,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
Time 10 %B 75
Compound name: tR(Retention time, σ total (s)
min)
3-nitrophenol 4,06 0,95

Propiophenone 5,81 1,00

benzophenone 7,50 0,95

Flow rate 2,0 Injection 3,0 Column 100

(ml/min) Volume(μL) Lenght
Time 10 %B 95

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 3,60 0,82

Propiophenone 4,99 0,85

benzophenone 6,28 0,81

Flow rate 2,0 Injection 3,0 Column 100
(ml/min) Volume(μL) Lenght
Time 10 %B 95

Compound name: tR(Retention time, σ total (s)

min)
3-nitrophenol 3,51 0,80

Propiophenone 4,83 0,82

benzophenone 6,05 0,79