Microbiology

Study Guide - Lab Exam #2

Know:
Answers to questions in the lab assignment schedule

For Aerotolerance
Media/apparatus used to test for aerotolerance
What enzymes aerobic microbes must produce to tolerate oxygen and why

For Phenol Red Broth (Carbohydrate Fermentation).

3 different carbohydrates we tested for
How to interpret results as shown on table 5-2 & fig. 5-4
Enzymes microbes must produce to breakdown either lactose or sucrose (see fig. 5-3)

For Methyl Red and Voges-Proskauer

What each of these tests are used (or designed) for
Examples of mixed acid fermentation by-products
Reagents used to produce a color change & interpretation of results (table 5-4)
Know Figures 5-3, 5-4, 5-5, 5-7, 5-10 & 5-11

SIM, Citrate, Lysine/Iron & Nitrate Agars

Purpose of each test and interpretation of results
Reagents used to test for positives and by-products they react with
What motility means and how a positive looks for motility
How to detect hydrogen sulfide gas (H2S) production
Review LIA handout
Know Figures 5-21, 5-23, 5-24, 5-25, 5-26, 5-26, 5-27 & LIA on Blackboard (under Labs)

Starch & Gelatin Hydrolysis, Catalase, Oxidase, Urease and Lipase Test
Know what tests are used for, what enzymes positive organisms produce and what they are
used for, how you determine a positive reaction (ie. Addition of indicators) and how each
positive looks.
Know figures 5-14, 5-15, 5-19, 5-20, 5-39, 5-40, 5-41, 5-45, 5-49, 5-50, 5-52, 5-53, 5-54, 5-
55, 5-56, 5-57 & 5-58