dilutions absorbance # of bacteria

Original E.coli 0.066 52,800,000

1/2 0.029 23,200,000
1/4 0.017 13,600,000
1/8 0.009 7,200,000
1/16 0.003 2,400,000

1OD = 8.00E+08 celulas/ml

FORMULA
# bacteria = absorbance * 1 OD

Numero de bacterias
60,000,000

50,000,000

40,000,000

30,000,000

20,000,000

10,000,000

0
0.000 0.010 0.020 0.030 0.040 0.050 0.060 0.070
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