Qeas Gmeestho
DETERMINACION DE PROTEINA POR EL METODO DE KJELDAHL
1. Pesat con la mayor exactitud disponible 025¢-#8-g de la muestra en un papel filtro 0
vidrio reloj; transferir la muestra a un tubo de digestién Kjeldahl de 250 mL (que debe
estar perfectamente limpio y seco) y agregar 6 g catalizador Kjeldahl y 15 mL de H:SO.
concentrado.
2. Inclinar el tubo (teniendo en cuenta que la boquilla no se encuentre diigida al analista)
y calentar suavemente hasta que cese la produecién de espuma. Llevar a cabo la
digestién hasta que la muestra esté completamente clara, libre de materia orginica y de
vez en cuando girar el balén para recoger cualquier material carbonizado adherido a la
pared. 440°C:
Nota: en el laboratorio este procedimiento seré Uevado a cabo a través de placas de calefacciin
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