Phase 4

DEVELOPMENT OF NEW PRODUCTS

Preparado por
JADER MARTINEZ C.C 1113622394

GRUPO
216005_3

PRESENTADO A
ZAIN SANCHEZ

UNIVERSIDAD NACIONAL ABIERTA Y A DISTANCIA UNAD

CEAD PALMIRA
ESCUELA DE CIENCIAS BÁSICAS TECNOLOGÍA E INGENIERÍA
6/05/2019

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2. Questionnaire for the Report about the simulation performed:

a. According to the results the table on the concentration of micro-organisms in Conc

(log10 UFC/g), how long it is expected that the Brochothrix thermosphacta, extent a
concentration of 7.66 log CFU/g?

R=/ In 20 hours Brochothrix thermosphacta reaches a concentration of 7.66 log CFU / g

b. Specify the length of time in which maintains a constant concentration of microorganisms

in Conc (log10 CFU/g)?

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R=/ In 171.8 hours the microorganisms remain constant from 27.8 hours to 199.6 hours.

c. To maintain the conditions given, if it is the only change to lower the temperature of
20ºC to 10ºC, and 20ºC to 5ºC the number of Brochothrix thermosphacta will
increase in the number of UFC/g.

R=/ Changing the temperature from 20 ° C to 10 ° C will take longer to maintain a constant
concentration

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changing the temperature from 20 ° C to 5 ° C will take even longer to maintain a constant
concentration.
It is concluded that for the microorganisms to duplicate in a fast way it is necessary to
increase the temperature

d. According to the kinetic results in the case of Brochothrix thermosphacta at 20 ºC,

10ºC and 5ºC, and taking into account that the doubling time, corresponds to the time
required for the microorganisms under certain conditions to be multiplied by two;
Taking into account the above, how much time is required to double the number of
micro-organisms, in each of these three cases?. Analyze and conclude with
arguments the obtained data.

At 20 ºC it takes 11.2 hours to double the microorganisms:

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It can be observed that in 3.2 hours the concentration of microorganisms is 3.2 (log10 CFU

/g

It can be observed that in 14.4 hours the concentration of microorganisms is 6.41 (log10
CFU / g) bone are doubled

At 10 ºC it takes 30.4 hours to double the microorganisms:

It can be seen that in 1.6 hours the concentration of microorganisms is 3.02 (log10 CFU / g)

It can be seen that in 32 hours the concentration of microorganisms is 6.04 (log10 CFU / g)
bone are doubled

At 5 ºC it takes 72 hours to duplicate the microorganisms

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It can be seen that from the hour 0 the concentration of microorganisms is 3 (log10 CFU / g)

It can be observed that in 72 hours the concentration of microorganisms is 6 (log10 CFU / g)

bone are doubled

It is concluded that microorganisms take longer to duplicate at lower temperatures

3. Simulation. Proceed to perform the prediction for each one of the conditions of
the following table:

Take a screen shot of the step by step, the implementation and the results of the
prediction of each one of the conditions laid down in the table. Analyze and
conclude with arguments the obtained data.

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The data is entered to analyze the Bacillus cereus .

It is observed that in 3.2 hours the concentration of microorganisms is 3.25 (log10 CFU / g)

In 6.6 hours the concentration of microorganisms is doubled, these are 6.51 (log10 CFU / g)

In 20 hours the concentration of microorganisms is at a constant level, this is 7.61 (log10

CFU / g)

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Enter the data to analyze Clostridium botulinum

It is observed from 0 hours the concentration of microorganisms is 3 (log10 CFU / g)

In 20.4 hours the concentration of microorganisms is doubled, these are 6 (log10 CFU / g

In 25 hours the concentration of microorganisms is 7.09 (log10 CFU / g

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The data are entered to analyze the Listeria monocytogenes

It is observed from 4 hours the concentration of microorganisms is 3.37 (log10 CFU / g)

At 11.8 hours the concentration of microorganisms doubles, these are 6.7 (log10 CFU / g)

In 30 hours the concentration of microorganisms is at a constant level, this is 8.52 (log10

CFU / g

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Data is entered to analyze Staphylococcus aureus

It is observed from 1.2 hours the concentration of microorganisms is 3.02 (log10 CFU / g)

In 10 hours the concentration of microorganisms doubles, these are 6.05 (log10 CFU / g

In 40 hours the concentration of microorganisms is at a constant level, this is 8.09 (log10

CFU / g)

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 BIBLIOGRAPHY
Guide for the development of the practical component with technological support -
ComBase simulator recuperado de
https://campus08.unad.edu.co/ecbti47/mod/folder/view.php?id=1285

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