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Abstract p90 Ribosomal S6 Kinase (RSK) have emerged as attractive targets for the
design of anticancer drugs. 3D-QSAR (comparative molecular field analysis
(CoMFA) and comparative molecular similarity indices analysis (CoMSIA)) studies
were performed on a series of 4- (pyridine-3-yl) phenol as RSK inhibitors. The
CoMFA and CoMSIA models using 16 compound inhibitors in the training set gave
Q2 values of 0.597 and 0.563,and R2 values of 0.993 and 0.990, respectively. The
adapted alignment method with the suitable parameters resulted in reliable models.
The contour maps produced by the CoMFA and CoMSIA models were employed to
rationalize the key structural requirements responsible for the activity. Based on the
3D-QSAR and docking results, a set of new molecules with high predicted activities
were designed.
1. Introduction
p90 ribosomal S6 kinase is initially referred to as ribosomal S6 kinase
(S6K). The identification of two kinase protein kinases 85-90 kDa (S6KI and
S6KII) with biochemical purification causes cloning of cDNA to encode
homologous proteins which are then renamed p90 ribosomal S6 kinase. The
family of RSK proteins comprises a much needed serine/ trionin kinase group
downstream of the Ras-MAPK line and regulates various cellular processes such
as cell growth and motility, cell proliferation and cellular viability [1]. RSK
kinases have an unusual structure in which an N-terminal kinase responsible for
the phosphorylation of RSK’s described substrates is fused to a C-terminal kinase
that seems to be dedicated to activating the N-terminal RSK kinase. The current
understanding of the activation scheme suggests that ERK (MAPK)
phosphorylates and activates the C-terminal kinase, which then creates a
phosphorylated docking site for PDK1 to bind to and activate the N-terminal
kinase. This activation scheme has been accepted as canonical, but additional
activating phosphorylation events have been described and may transfer the strict
control of RSK activity from the MAP kinase signaling pathway to other
signaling pathways [2,3].
A Pyridine series of 4- (pyridine-3-yl) phenol with RSK inhibitory
activities were reported. These 4- (pyridine-3-yl) phenol, with excellent RSK
inhibitory activities. [4]. In the present study, three-dimensional quantitative
structure-activity relationship (3D-QSAR) methods along with used to explore the
structure-activity relationship (SAR) of these 4- (pyridine-3-yl) phenol. 3D-
QSAR methods, comparative molecular field analysis (CoMFA) and comparative
molecular similarity indices analysis (CoMSIA), were performed to foresee the
activities of these 4- (pyridine-3-yl) phenol and offered the regions where
interactive fields (steric, electrostatic, hydrophobic, hydrogen bond donor and
hydrogen bond acceptor fields) may increase or decrease the activity. These
developed models can help understanding the SAR of 4- (pyridine-3-yl) phenol
and can also serve as a valuable guide for the design of novel inhibitors with
robust potency. Furthermore, we have designed a number of new 4- (pyridine-3-
yl) phenol derivatives by utilizing the structure information obtained from the
CoMFA and CoMSIA models, which exhibit excellent predictive potencies.
Moreover, the predicted activities of these newly designed molecules may be
reliable.
R2
N
Table 1. The structures of the training and test set molecules.
N
OH
F F
OH
OH
2 F 0.050 7.301
N
OH
F F
3 F 0.060 7.2218
N
OH
F F
O
4* F O 0.13 6.8861
N
OH
F F
NH
5 F NH 2.1 5.6778
N
OH
F F
6 F 0.78 6.1079
N
OH
F F
7* F 0.032 7.4948
N
OH
F F
8 F 0.046 7.3372
O O
N
OH
F F
10 F N 0.005 8.301
N
H N
N NH
OH
F F
14 F 0.007 8.1549
N
OH
F F
O
O
15 F 0.008 8.0969
N
O OH
F F
O
N
16* F H N
H
0.020 7.699
N
OH
F F
NH NH
N N
17 F 0.006 8.2218
N
OH
N F F
N
N
N
18 F 0.005 8.301
N
OH
O F F
O
N
N
19 F 0.004 8.3979
N
O OH
F F
O
20 F N 0.080 7.0969
N
N
OH
F Cl Cl
F
21 Cl 4.8 5.3188
Table 2. The actual pIC50s, predicted pIC50s (Pred.) and their residuals (Res.) of the
training and test set molecules.
pIC50 Prediksi (µM)
Senyawa pIC50 (µM)
CoMFA Residual CoMSIA Residual
1* 6.7447 6.758 -0.0133 6.7422 0.0025
2 7.301 7.3982 -0.0972 7.2862 0.0148
3 7.2218 7.3126 -0.0908 7.4977 -0.2759
4* 6.8861 6.8864 -0.0003 6.9013 -0.0152
5 5.6778 5.7053 -0.0275 5.6856 -0.0078
6 6.1079 6.083 0.0249 6.0665 0.0414
7* 7.4948 7.457 0.0378 7.4699 0.0249
8 7.3372 7.2449 0.0923 7.406 -0.0688
9 8.3979 8.3551 0.0428 8.3535 0.0444
10 8.301 8.3043 -0.0033 8.3818 -0.0808
11* 7.7447 7.784 -0.0393 7.7836 -0.0389
12 8.3979 8.4429 -0.045 8.3292 0.0687
13 7.0269 6.7808 0.2461 6.9942 0.0327
14 8.1549 8.1679 -0.013 7.9425 0.2124
15 8.0969 8.0884 0.0085 8.1118 -0.0149
16* 7.699 7.684 0.015 7.6723 0.0267
17 8.2218 8.2688 -0.047 8.1833 0.0385
18 8.301 8.2658 0.0352 8.3686 -0.0676
19 8.3979 8.393 0.0049 8.3812 0.0167
20 7.0969 7.1539 -0.057 7.0584 0.0385
21 5.3188 5.3931 -0.0743 5.3114 0.0074
Test set molecules.
OH
(r2pred)= (SD-PRESS)/SD
In this equation, SD is the sum of the squared deviations between the inhibitory
activities of the test set and the mean activity of the training molecules and
PRESS is the sum of squared deviations between predicted and actual activity
values for each molecule in the test set [15]
3. Results and Discussion
3.1 CoMFA and CoMSIA Models
The statistical parameters for the CoMFA and CoMSIA models are given
in Table 3. For the CoMFA model, partial least squares (PLS) regression
produced a excellent cross-validated correlation coefficient (Q2) of 0.597 (>0.5)
with an optimized component of 6, which suggesting that the model is reliable
and it should be a useful tool for predicting the IC50 values. The non cross-
validated PLS analysis gave a high correlation coefficient (R2) of 0.993, F value
of 221.414 and a low standard error estimate (SEE) of 0.108. The contributions of
steric and electrostatic fields to this model were 0.513 and 0.487, respectively.
The external validation correlation coefficient (r2pred) value based on molecules of
the test set was 0.996 for the CoMFA model. The actual and predicted pIC50
values of the training set and test set by the model are given in Table 2. The
relationship between actual and predicted pIC50 of the training set and test set
compounds of the CoMFA model is illustrated in Figure 3a, where almost all
points are located on the diagonal line.
Figure 3. Graph of actual versus predicted pIC50 of the training set and the test set
using CoMFA (a) and CoMSIA (b).
♦ Training (a) ♦ Training (b)
♦ Test 9 ♦ Test
9
8.5 8.5
8
Predicted pIC50 CoMFA
For the CoMSIA model, the statistical parameters revealed that steric,
electrostatic, hydrophobic, hydrogen bond donor and acceptor features
significantly influence the activity of the inhibitors. The CoMSIA model gave a
cross-validated correlation coefficient (Q2) of 0.563 (>0.5) with an optimized
component of 3, which suggested that the model is reliable and should be a useful
tool for predicting the IC50 values. The non cross-validated PLS analysis gave a
high correlation coefficient (R2) of 0.99, F value of 153.383 and a low error
estimate (SEE) of 0.129. The contributions of steric, electrostatic, hydrophobic,
hydrogen bond donor and acceptor fields were 0.162, 0.343, 0.170, 0.228 and
0.096, respectively. The predictive correlation coefficient (r2pred) value based on
molecules of the test set was 0.996 for the CoMSIA model. The actual and
predicted pIC50 values and residual values for training set and test set compounds
are given in Table 2. The graph of actual activity versus predicted pIC50 of the
training set and test set is illustrated in Figure 3b, where almost all points are
located on the diagonal line.
The electrostatic field contour maps of CoMFA and CoMSIA are shown
in Figure 5a and b, respectively. Compound 18 was selected as a reference
molecule again. The electrostatic field is indicated by blue and red contours,
which demonstrate the regions where electron-donating group and electron-
withdrawing group would be favorable, respectively. For CoMFA, the blue
contours near the position of R2 show that subtituents with electropositive
charges can increase activity. This explains why the compounds 1 (R2 = p-
cresol), 2 (R2 = p-cresol), and 3 (R2 = toluene) with IC50 values respectively
(0.018 μM), (0.050 μM), and (0.060 μM) has a lower activity than the compound
18 (R2=1-methyl-4-p-tolylpiperazine) having greater electropositive properties
with IC50 (0.005 μM) activity and the compound 19 (R1=F and R2=4-p-
tolylmorpholine) which has an IC50 value (0.004 μM). For CoMSIA, Blue
contours show subtituents with this electropositive charge will increase activity
and red contours show subtituents with electronegative charges will increase
activity. This may explain that compound 18 has better activity than compounds
1-8, 11, and 20-21.
(a) (b)
(a) (b)
(c)
Figure 6. Contour maps of CoMSIA analysis in combination with
compound 18. Hydrophobic fields (a), the yellow and white contours
(80% and 20% contributions) indicate favorable and unfavorable
hydrophobic groups; Hydrogen bond donor contour map (b), the cyan
and purple contours (80% and 20% contributions) indicate favorable
and unfavorable hydrogen bond donor groups; Hydrogen bond
acceptor contour map (c), the magenta and red contours (80% and
20% contributions) indicate favorable and unfavorable hydrogen bond
acceptor groups. Compound 18 is depicted in ball and stick
representation, colored by atom type (white C, blue N, red O, cyan H).
CoMSIA analysis on the magenta contour bond acceptor area around the
benzene R2 substituent of the hydrogen bond acceptor indicates a potential
increase in activity while the red contour indicates a potential decrease in activity
as in Figure 6b. CoMSIA analysis on the hydrogen contour hydrogen donor donor
field around the OH substituent of the hydrogen bond donor has the potential to
increase activity while the purple contour area indicates the hydrogen bond donor
substituent decreases the activity as shown in compound 18 in figure 6c.
Table 4. Structure, predicted pIC50 values, and surflex-dock total score of the
newly designed molecules.
OH
F F
N
N R2
R1
Hydrogen bond
Hydrogen bond binding region OH binding region
R1 R1
R2
N
Hydrogen bond
binding region Bulky, electron-donating, hydrophobic, and
hydrophilic, and substituent favoured region
Hydrogen bond binding region
8.8
8.7
CoMFA
8.6
Predict pIC50 by CoMFA and CoMSIA
8.5 CoMSIA
8.4
8.3
8.2
8.1
8
7.9
7.8
7.7
HA5
HA1
HA2
HA3
HA4
HA6
HA7
HA8
HA9
18
HA10
HA11
HA12
HA13
HA14
HA15
Compound
12
HA28
18
HA16
HA17
HA18
HA19
HA20
HA21
HA22
HA23
HA24
HA25
HA26
HA27
HA29
HA30
Compound
4. Conclusion
Physico-chemical pretread that affects breast anticancer activity of
pyridine derived compounds as inhibitors p90 Ribosomal S6 Kinase II (RSK II) is
a steric, electrostatic and hydrophobic parameter based on contour map
interpretation. There are 11 other design compounds that are predicted to have
better activity than the experimental 18 compounds based on the amount of pIC50
CoMFA and CoMSIA predictions obtained.
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