LEMBAR PERHITUNGAN DATA

1. Sebelum Inkubasi
F = 16,5 ml
a. Variabel 1
M = 12 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16,5−12) 𝑥 𝑥
5 5
= x 2,5
20
= 11,25 mg/ml
b. Variabel 2
M = 14,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16,5−14,5) 𝑥 𝑥
5 5
= x 2,5
20
= 5 mg/ml
c. Variabel 3
M = 13,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16,5−13,5) 𝑥 𝑥
5 5
= x 2,5
20
= 7,5 mg/ml
d. Variabel 4
M = 14,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16,5−14,5) 𝑥 𝑥
5 5
= x 2,5
20
= 5 mg/ml
2. Setelah Inkubasi
F = 16 ml
a. Variabel 1
 t = 10 menit
 M = 13 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−13) 𝑥 𝑥
5 5
= x 2,5
20
= 7,5 mg/ml
 t = 25 menit
 M = 12,6 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−12,6) 𝑥 𝑥
5 5
= x 2,5
20
= 8,5 mg/ml
 t = 40 menit
 M = 12 ml
 𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−12) 𝑥 𝑥
5 5
= x 2,5
20
= 10 mg/ml
 t = 55 menit
 M = 10,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−10,5) 𝑥 𝑥
5 5
= x 2,5
20
= 13,75 mg/ml
 t = 70 menit
 M = 10 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−10) 𝑥 𝑥
5 5
= x 2,5
20
= 15 mg/ml
b. Variabel 2
 t = 10 menit
 M = 13,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−13,5) 𝑥 𝑥
5 5
= x 2,5
20
= 6,25 mg/ml
 t = 25 menit
 M = 11,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−11,5) 𝑥 𝑥
5 5
= x 2,5
20
= 11,25 mg/ml
 t = 40 menit
 M = 11 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−11) 𝑥 𝑥
5 5
= x 2,5
20
= 12,5 mg/ml
 t = 55 menit
 M = 9,8 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−9,8) 𝑥 𝑥
5 5
= x 2,5
20
= 15,5 mg/ml
 t = 70 menit
 M = 9,2 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
 20 25
(16−9,2) 𝑥 𝑥
5 5
= x 2,5
20
= 17 mg/ml
c. Variabel 3
 t = 10 menit
 M = 12,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−12,5) 𝑥 𝑥
5 5
= x 2,5
20
= 8,75 mg/ml
 t = 25 menit
 M = 11,8 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−11,8) 𝑥 𝑥
5 5
= x 2,5
20
= 10,5 mg/ml
 t = 40 menit
 M = 11,2 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−11,2) 𝑥 𝑥
5 5
= x 2,5
20
= 12 mg/ml
 t = 55 menit
 M = 9,5 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−9,5) 𝑥 𝑥
5 5
= x 2,5
20
= 16,25 mg/ml

 t = 70 menit
 M = 9,3 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−9,3) 𝑥 𝑥
5 5
= x 2,5
20
= 16,75 mg/ml
d. Variabel 4
 t = 10 menit
 M = 14 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−14) 𝑥 𝑥
5 5
= x 2,5
20
= 5 mg/ml
 t = 25 menit
 M = 13,2 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
 20 25
(16−13,2) 𝑥 𝑥
5 5
= x 2,5
20
= 7 mg/ml
 t = 40 menit
 M = 12,8 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−12,8) 𝑥 𝑥
5 5
= x 2,5
20
= 8 mg/ml
 t = 55 menit
 M = 11,2 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−11,2) 𝑥 𝑥
5 5
= x 2,5
20
= 12 mg/ml
 t = 70 menit
 M = 10,8 ml
𝑉𝑡𝑜𝑡𝑎𝑙 𝑉𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀) 𝑥 𝑥
𝑉𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉𝑑𝑖𝑎𝑚𝑏𝑖𝑙
C = x 2,5
𝑉 𝑡𝑜𝑡𝑎𝑙
20 25
(16−10,8) 𝑥 𝑥
5 5
= x 2,5
20
= 13 mg/ml
3. Aktivitas Enzim
a. Variabel 1
 t = 10 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
11,25−7,5 1000
= 10 x 180
= 2,083 unit/ml menit
 t = 25 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
11,25−8,5 1000
= 25 x 180
= 0,61 unit/ml menit
 t = 40 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
11,25−10 1000
= 40 x 180
= 0,174 unit/ml menit
 t = 55 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
13,75−11,25 1000
= x 180
55
= 0,25 unit/ml menit
 t = 70 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
15−11,25 1000
= 70 x 180
= 0,298 unit/ml menit
b. Variabel 2
 t = 10 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
6,25−5 1000
= 10 x 180
= 0,694 unit/ml menit
 t = 25 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
11,25−5 1000
= 25 x 180
= 1,38 unit/ml menit
 t = 40 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
12,5−5 1000
= 40 x 180
= 1,042 unit/ml menit
 t = 55 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
15,5−5 1000
= 55 x 180
= 1,06 unit/ml menit
 t = 70 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
17−5 1000
= 70 x 180
= 0,952 unit/ml menit
c. Variabel 3
 t = 10 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
8,75−7,5 1000
= 10 x 180
= 0,694 unit/ml menit
 t = 25 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
10,5−7,5 1000
= 25 x 180
= 0,6 unit/ml menit
 t = 40 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
12−7,5 1000
= 40 x 180
= 0,625 unit/ml menit
 t = 55 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
16,25−7,5 1000
= 55 x 180
= 0,883 unit/ml menit
 t = 70 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
16,75−7,5 1000
= 70
x 180
 = 0,734 unit/ml menit
d. Variabel 4
 t = 10 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
5−5 1000
= 10 x 180
= 0 unit/ml menit
 t = 25 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
7−5 1000
= 25 x 180
= 0,4 unit/ml menit
 t = 40 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
8−5 1000
= 40 x 180
= 0,416 unit/ml menit
 t = 55 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
12−5 1000
= 55 x 180
= 0,7 unit/ml menit
 t = 70 menit
𝐶𝑠𝑒𝑡𝑒𝑙𝑎ℎ−𝐶𝑠𝑒𝑏𝑒𝑙𝑢𝑚 1000
AE = x
𝑇 𝐵𝑀
13−5 1000
= x
70 180
= 0,635 unit/ml menit
 DATA PENDUKUNG
a. Sebelum inkubasi
F = 16,5 ml
Data Analisa
Sebelum Inkubasi t0
M 12 ml
Variabel 1
C 11,25 ml
M 14,5 ml
Variabel 2
C 5 ml
M 13,5 ml
Variabel 3
C 7,5 ml
M 14,5 ml
Variabel 4
C 5 ml

b. Setelah inkubasi
F = 16 ml

Data Analisa
Setelah Inkubasi t 10 menit t 25 menit t 40 menit t 55 menit t 70 menit
M 13 ml 12,6 ml 12 ml 10,5 ml 10 ml
13,75
C 7,5 mg/ml 8,5 mg/ml 10 mg/ml 15 mg/ml
Variabel mg/ml
1 2,083 0,61 0,174 0,25 0,298
AE unit/ml unit/ml unit/ml unit/ml unit/ml
menit menit menit menit menit
M 13,5 ml 11,5 ml 11 ml 9,8 ml 9,2 ml
6,25 11,25 12,5 15,5 17
C
Variabel mg/ml mg/ml mg/ml mg/ml mg/ml
2 0,694 1,38 1,042 1,06 0,952
AE unit/ml unit/ml unit/ml unit/ml unit/ml
menit menit menit menit menit
M 12,5 ml 11,8 ml 11,2 ml 9,5 ml 9,3 ml
8,75 10,5 16,25 16,75
C 12 mg/ml
Variabel mg/ml mg/ml mg/ml mg/ml
3 0,694 0,6 0,625 0,883 0,734
AE unit/ml unit/ml unit/ml unit/ml unit/ml
menit menit menit menit menit
 M 14 ml 13,2 ml 12,8 ml 11,2 ml 10,8 ml
13
C 5 mg/ml 7 mg/ml 8 mg/ml 12 mg/ml
Variabel mg/ml
4 0,416 0,635
0 unit/ml 0,4 unit/ml 0,7 unit/ml
AE unit/ml unit/ml
menit menit menit
menit menit