LNCAP CLONE FGC - RPMI 1640 Modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM

sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate + 10% soro fetal bovino.

SHSY5Y - 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium Modified to contain
Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate,
and 1500 mg/L sodium bicarbonate and F12 Medium + 10% soro fetal bovino.

A549 - F-12K Modified to contain 2 mM L-glutamine and 1500 mg/L sodium bicarbonate + 10% soro
fetal bovino.

Jurkat, clone E6-1 - RPMI-1640 Medium Modified to contain 2 mM L-glutamine, 10 mM HEPES, 1

mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate + 10% soro fetal
bovino.

Molt-4 - RPMI-1640 Medium Modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium

pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate + 10% soro fetal bovino.

K562 - RPMI-1640 Medium Modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium

pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate + 10% soro fetal bovino.

IMR-90 - Eagle's Minimum Essential Medium Modified to contain Earle's Balanced Salt Solution, non-
essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate
+ 10% soro fetal bovino.

MCF-7 - RPMI-1640 Medium Modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium

pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate + 20% soro fetal bovino.

- HeLa - DMEM + non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and

1500 mg/L sodium bicarbonate + 10% soro fetal bovino.

- SCC-25 - A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium
containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM
sodium pyruvate and supplemetned with 400 ng/ml hydrocortisone and 10% fetal bovine
serum

- C3A - DMEM + non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and

1500 mg/L sodium bicarbonate + 10% fetal bovine serum

- HT-29 - DMEM + 2 mM L-glutamine, and 1500 mg/L sodium bicarbonate + 10% fetal
bovine serum