Water is a very important part of life.

In determining the quality of water water quality

standards in Indonesia have been established, both quality standards for drinking water, industrial
water, etc. The purpose of this practicum is to be able to know the influence of media types on the
number of colonies, be able to determine growth rate and doubling time of colony growth, and be
able to compare the radius of microbial development with disinfectants with different
concentrations using water samples from Tugu Muda.
Natural waters have dynamic properties and a continuous flow of energy. This occurs as
long as the system inside does not get disturbances or obstacles, including in the form of pollution.
According to the regulation of the Minister of Health of the Republic of Indonesia, there are several
requirements for clean water and drinking water, including biological, physical and chemical
requirements. Microbial colonies have several properties that are divided into 2, namely general
properties and special properties. There are several factors that affect microbes, namely regulating,
medium, the influence of light, mechanical, chemical as well as pH. Calculation of colonies formed
can use SPC as well as manual calculations.
The materials used in this practicum are water samples from the University of Diponegoro,
aquadest, media, and disinfectants. The tools used are beaker glass, petridish, erlenmeyer, stirrer,
electric stove, and drop pipette. Before starting the water check, a preliminary step is taken, namely
preparing the media to be used and dividing the media into petridihs evenly. Colony testing was
done by preparing the media, sprinkling samples and incubating 2 days. Whereas for the
disinfecting test, it was done by preparing the media, dripping disinfectants and incubating 2 days.
Based on the water examination practicum data, the media with the most growth of the
colonies is PDA media. Carbohydrate-rich PDAs have the highest growth rates. The higher the
concentration of disinfectant the further the radius of the development of microorganisms.
The advice from this lab is to experiment with a device that was sterilized first. Check in
calculating the number of colonies, and make sure the sample is dropped on PDA media or so that
it is still semi-solid.