TCVN TIEU CHUAN QUOCGIA TCVN 10482 : 2014 ISO 22959 : 2009 Xudt ban [an 4 DAU MG DONG VAT VA THU VAT - XAC BINH HYDROCACBON THOM DA VONG BANG SAC KY PHUC CHAT CHO-NHAN TRUC TIEP VA SAC KY LONG HIEU NANG CAO (HPLC) CO DETECTOR HUYNH QUANG Animal and vegetable fats and oils — Determination of polycyclic aromatic hydrocarbons by on-line donor-acceptor complex chromatography and HPLC with fluorescence detection HA NOI - 2014TCVN 10482:2014 Loi néi dau TCVN 10482:2014 hoan toan turong durong vai ISO 22959:2009; TCVN 10482:2014 do Ban ky thuat tiéu chudn quéc gia TCVNITC/F2 Déu mé déng vat va thuc vat bién soan, Téng cuc Tiéu chudn Bo luéng Chat lrong thdm dinh, B6 Khoa hoc va Céng nghé- céng bé.TCVN 10482:2014 Loi gidi thigu Cc hydrocacbon thom da véng (PAH) dtrga tao thanh trong suét qué tinh nhiét phan nhw qué tinh dét chay kh6ng hoan toan cdc cht hu co ho&c cdc chdt cd ngudn géc tram tich (dau khodng). Dau m6 thu phdm cé thé bj nhi&m ban do 6 nhiém méi trurémg va/hoac cdc bute xir ly truéc Khi tinh luyén. Céc PAH trong ddu ma la méi qh’ ‘rigal ‘vé sire khde do c6 kha nding gay ung thu. Ham lung khac nhau ola céc PAH 68 due tim théy trong dau thye phdm thd. Qué trinh tinh luygn dau (khé mii, tay trang, xi ly than) theo cdc aidu kién thich hop Iam gidm ham lung cdc PAH riéng ré dén miro microgram trén kilogram. C4c phuong php phan tich PAH trong dau mo thye vat da durge biét dén bao gém cdc quy trinh tach chiét khé va phirc tap, quy trinh tinh sach a8 téch cac PAH ¢6 ham lugng thdp. V6i phuong phap s4c ki phtrc cht cho nhan (DACC) thi PAH 06 thé durge chiét ra khdi céc chdt nén mu khdc nhau. Céc PAH Id céc phan tir cho electron (-electron) va sy tong tac manh cla c&c PAH v6i mét phan tir nhan electron trong pha tinh lam gir lai c4¢ PAH va riza giai (phan I6n) cae thanh phan khdc trong dau. Ti@u chuan nay quy dinh phurong phap tryc tiép ty dong 48 xc dinh c&c PAH trong dau mé thy phdm, phuong phap nay c6 thé d& dang sir dung nhu phuong phdp phan tich thong dung. Phuong phap nay gdm cé cbt DACC tinh sach ghép di LC-LC véi c6t phan tich ding cho qua trinh tach chiét. Ce PAH durge dinh lugng bang detector huynh quang. ‘So sdnh véi cdc phuong phdp cdi thl phuong phdp ty dgng trc tiép nay lam gidm dang ké ham long dung mdi si dung va tiét kiém thdi gian dang ké. Lam sach ct DACC nhanh va tién hanh trong suét qua trinh chay HPLC mu truréc 46. Téng thdi gian phan tich mt mau khodng 90 min, so voi cdc phuong phép truyén théng can tir 8 h dén 10 h. Ngoai ra, hé théng o6 thé chay lién tye trong ngay. Cudi cing, hao hyt ce PAH dé bay hoi trong suét qué trinh bay hoi dung méi dug loai triv. Giéi han dinh Ivong 0,1 pa/kg cia cdc PAH riéng [6 thu dugc bang phuong phap DACC ty déng higu chinh cac hé sé thu hdi c6 thd khéng hoan toan tir cdc mAu higu chudn dugc xv ly tong ty nhu mu phan tich. He théng nay sir dung thiét bi HPLC thong thureng.