15167016
rips www uso nero esfE3500-77hate-shoet
USBiological Technical Data
Life Sciences
£3500-27
Clone Type
Polyclonal
Host
Goat
Isotype
los
Grade
Afinity Purified
‘Applications
E
Crossreactivity
ec
Shipping Temp
Blue lee
Storage Temp
20°C
Goat Anti-Escherichia coli, 0157:H7 (E. coli)
Escherichia coli (E. coli) is a Gram negative bacterium that is
‘commonly found in the lower intestine of warm-blooded
organisms (endotherms). Most E. coli strains are harmless, but
some, such as serotype 0157:H7, can cause serious food poisoning
in humans, and are occasionally responsible for costly product
recalls. The harmless strains are part of the normal flora of the gut,
and can benefit their hosts by producing vitamin K2, or by
preventing the establishment of pathogenic bacteria within the
intestine. €, coli are not always confined to the intestine, and their
ability to survive for brief periods outside the body makes them an
ideal indicator organism to test environmental samples for fecal
contamination. The bacteria can also be grown easily and its
genetics are comparatively simple and easily-manipulated or
duplicated through a process of metagenics, making it one of the
best-studied prokaryotic model organisms, and an important
species in biotechnology and microbiology. E. coli was discovered
by German pediatrician and bacteriologist Theodor Escherich in
1885, and is now classified as part of the Enterobacteriaceae family
of gamma-proteobacteria.
Applications
Suitable for use in ELISA. Other applications not tested,
Recommended Dilutions
‘Optimal dilutions to be determined by the researcher
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and
thawing, Store at-20°C Aliquots are stable for 12 months after receipt. For
maximum recovery of product, centrifuge the original val after thawing and
prior to removing the cap.
Immunogen
E.coli serotype O157:H7
Form
Supplied as a quid in PBS, 0.09% sodium azide
Purity
Purified by affinity chromatography.
Specificity
Recognizes E, coll O157:H7,
ipsshwwnw usiorelasbaciosf3500-27ala sheet 1215167016 tps uso neni esf3500-77hate-sheet
Intended for research use only. Not for use in human, therpeutic, or diagnostic applications.
References
General References:|1. "Escherichia coli 0157:H7", CDC Division of Bacterial and Mycotic Diseases, Retrieved on 2007-01-25.2. Vogt RL,
Dippold L (2008). “Escherichia coli 0187:47 outbreak associated with consumption of ground beet, June-july 2002", Public Health Rep
120 (2): 174-8, PMID 15842119.|3. Bentley R, Meganathan R (01 September 1982). "Biosynthesis of vitamin K {menaquinone) in
bacteria”. Microbiol Rev. 46 (3): 241-80, PMID 6127606, PMC: 281544, |4, Hudault S, Guignot, Servin AL uly 2001), “Escherichia coli
strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection, Gut 49 (1): 47-55,
ddoi10.1136/gut.a9.1.47, PMID 11413110.[5, Reid 6, Howard J, Gan 85 (September 2001). “Can bacterial interference prevent infection?”
Trends Microbiol. § (9): 424-8, doi:10.1016/50966-842x(01)02132-1, PMID 11553454.16. Feng P, Weagant S, Grant, M (2002-09-01).
“Enumeration of Escherichia coll and the Coliform Bacteria". Bacteriological Analytical Manual (8th ed). FOA/Center for Food Safety &
Applied Nutrition, Retrieved on 2007-01-25. 17. Thompson, Andrea (2007-06-04). "E. cli Thrives in Beach Sands", Live Science. Retrieved
‘0n 2007-12403.|8. "Escherichia". Taxonomy Browser. NCBI. Retrieved on 2007-11-30. |9. Kubitschek HE (01 January 1980). "Cell volume
Increase in Escherichia col after shifts to richer media". Bacteriol. 172 (1): 94-101, PMID 2403552. PMC: 208405. 10. Madigan MT,
Martinko JM (2006). Brock Biology of microorganisms (11th ed), Pearson, ISBN 0-13-196893-9.|11. Fotadar U, Zaveloff P, Terracio L
(2005). “Growth of Escherichia col at elevated temperatures". Basic Microbiol. 45 (5): 403-4. dol:10.1002/jobm.200410542. PMID
16187264, |12. Ingledew W, Poole RK (1984). "The respiratory chains of Escherichia col", Microbiol Rev, 48 (3): 222-71. PMID
66387427. 13. Darnton NC, Turner L, Rojevsky 5, Berg HC, On torque and tumbling in swimming Escherichia coli. Bacteriol. 2007 Mar:18
Briissow H, Canchaya C, Hardt WD (September 2004). "Phages and the evolution of bacterial pathogens: from genomic rearrangements
'28/MMBR.68.3,560-602.2008, PMID 15353570, PMC: 515249.
to lysogenic conversion”. Microbiol. Mol. Biol. Rev. 68 (3): 560-602. dot:10.
United States Biological | 4 Technology Way | Salem, MA.01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net
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