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Hybridoma
Hybridoma
Monoclonal antibodies are antibodies derived from a single clone of plasma cell. All
have the same antigenic specificity i.e. they are produced against a single epitope of
the antigen
• Inject a mouse with antigen of desired epitope – mouse splenic B cells are
activated against this antigen – wait for an interval and harvest these splenic
B cells
2 – MYELOMA CELLS:
3 – FUSION:
• Mouse splenic B cells and mutated myeloma cells fused in polyethylene glycol
broth
1. Unfused splenic cells: they can grow – but they are not immortal – do not
survive long
2. Unfused myeloma cells: they cannot grow – they lack HGPRT – cannot
perform salvage pathway
• Medium containing the hybridoma cells is diluted and dispersed into multi well
plates
• Diluted to such an extent that each well contains only one cell
THERAPEUTIC USES:
DIAGNOSTIC USES: