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    Asadulhaq Ali Hamidi
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    AOCS Method

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    AOCS Cd 1d-92

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    AOCS Method

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
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    1K views3 pages

    AOCS CD 1d-92

    Uploaded by

    Asadulhaq Ali Hamidi
    AOCS Method

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 3
    SAMPLING AND ANALYSIS OF COMMERCIAL FATS AND OILS LOSS AOCS Official Method Cd 14-92 Respproved 2009 lodine Value of Fats and Oils Cyclohexane-Acetic Acid Method DEFINITION ‘The iodine valuc is a measure ofthe unsaturation of fas and oils and is expressed in terms of the number of centigrams of iodine absorbed per gram of test sample (% iodine absorbed), SCOPE Applicable to all normal fats and oils with iodine values in the range of 18-165 that do not contain conju- gated double bonds (sce Notes, 1). APPARATUS. 1. Ghass-stoppered iodine flasks —500 mL. 2. Glassstoppered volumetric flasks—1000 mL, for preparing standard solutions. 3, Pipee—25 mL, for accurately dispensing 25.0 mL of Wijs solution. 4, Volumetie dispenser—20 mL, I mL adjustability, for 10% potassium iodide (KI) solution. 5. Volumetric dispenser—2 mL, | ml. adjustability fr starch solution. 6. Volumetric dispenser—50 ml, 1 mL adjustability, for distilled water. Repeater pipet—wich filing flask, 20 ml. for cyclohexane. 8. Analytical balance—accurate to * 0.0001 g. 9. Magnetic stirrer. 10. Filter paper—Whatman no. 541, or equivalent. 1, Beakers—50 mL. 12, Hot air oven. 13, Timer. REAGENTS, 1. Wijssolution—sce Notes, 2 and Caution. 2: Potassium iodide (KI) solution—100 g/l. (10% solution), prepared by dissolving 100 g of reagent grade KI in 1000 ml. of deionized water. 3. Cyclohexane—reagent grade (see Notes, 3and Caution). 4, Glacial acetic acid—reagent grade (sce Notes, Cation). 5. Reagent for diluting test sample—prepared by mixing cyclohexane and glacial acetic acid, 1:1, v/v. The absence of oxidizable iatter in the reagent is verified by shaking 10 mL. of the reagent with 1 mL of saturated aqueous potassium dichromate solution and 2 ml. of concentrated sulfuric acid; no green coloration should appear 6, Hydrochloric acid—reagent grade (see Notes, Caution). 7. Soluble starch solution—recently prepared, tested for sensitivity (see Notes, 4). Make a paste with 1 g of natura, soluble starch (see Notes, 5) and a small amount of cold distilled water. Add, while stirring, co 100 mL. of boiling water. Tes for sensitiviry—Place 5 ml. of starch solution in 100 mL. of water and add 0.05 ml. of freshly prepared 0.1 M KI solu- tion and one drop of 50 ppm chlorine solution made by diluting ! mL of a commercial 5% sodium hypochlorite (NaC) solution to 1000 mL. The deep blue color produced must be discharged by 0.05 ml. of 0.1 N sodium thiosulfate. 8. Potassium dichromate—reagent grade. The potassium dichromate is finely ground and dried to constanc weight at about 110°C before using (see Notes, Caution). Sodium thiosulfate (Na,S,0,'5H,O)—0.1 M, accurately standardized, prepared from reagent-grade Na, Notes, 6). ‘Standardization of sodium chiosulfate—Weigh 0.16-.22 g of finely ground and dried potassium dichromate into a 500 ml. flask or bottle by difference from a weighing bore. Dissolve in 25 ml. of water, add 5 ml. of concentrated hydro- chlotic acid, 20 ml. of potassium iodide solution and rotate to mix. Allow to stand for 5 min, and then add 100 mL. of distilled water. Titrate with sodium ehiosulfae solution, shaking continuously until the yellow color has almost disappeared. ‘Add 1-2 mL of starch indicator and continue the titration, adding the thiosulfate solution slowly until the blue color just

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