APPENDICES

COMPUTATION FOR OSMOSIS

 SOLUTION A: NaCl solution (2M): Need 10 mL of 1M NaCl
C₁V₁ = C₂V₂
(2M)(V₁) = (1M)(10 mL)
2M V₁ = 10 M mL
2M 2
V₁ = 5 mL = 5 mL NaCl
+ 5mL H₂O

 SOLUTION B: NaCl solution (1M) : Need 10 mL of 0.1 M NaCl

C₁V₁ = C₂V₂
(1 M)(V₁) = (0.1 M)(10 mL)
1M V₁ = 1 M mL
1M 1
V₁ = 1 mL = 1 mL NaCl
+ 9 mL H₂O

COMPUTATION FOR DIALYSIS

 SOLUTION 1: 60 mL of 5% NaCl ; MW NaCl = 58g
C₁V₁ = C₂V₂
(2M) (V₁) = (5%) (60mL)
5% = 5g × 1,000 mL × 1 mol = 0.86 M
100 mL 1L 58g
(2M) (V₁) = (0.86M) (60 mL)
2M V₁ = 51.6 M mL
2M 2M
V₁ = 25.8 mL NaCl
+ 34.2 mL H₂O
 SOLUTION 3: 30 mL of 40% sucrose solution
40% = 5g × 30 = 12 g
100 mL
 ACTIVITY A: CHARACTERISTICS OF CELLS: SACCHAROMYCES CEREVISIAE AS MODEL
ORGANISM

HAS BEEN BOILED FOR

5-10 MINS

YEAST SUSPENSION A YEAST SUSPENSION B

1 DROP FOR EACH

SUSPENSION + 1 DROP
OF METHYLENE BLUE IN YEAST SUSPENSION A: YEAST SUSPENSION B:
A SLIDE PALER DARKER

BEFORE STORED INSIDE THE

LOCKER FOR 24 HRS AFTER 24 HRS
 ACTIVITY B: OSMOSIS

20 SECONDS 40 SECONDS 60 SECONDS

YEAST WITH SOLUTION A

20 SEC 40 SECONDS 60 SECONDS

ONDS

YEAST WITH H₂O

40 SECONDS 60 SECONDS
20 SEC

ONDS

YEAST WITH SOLUTION B

ACTIVITY C: DIALYSIS