INSTALLATION

Table of contents
General environment . . . . . . . . . . . . . . . . . . . . . . . 2
Celly 70 components . . . . . . . . . . . . . . . . . . . . . . . 3
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Instrument preparation . . . . . . . . . . . . . . . . . . . . . . 5
Access to fluidics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Control of fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Reagents and electric connections . . . . . . . . . . . . . . 8
Diluent (white connector). . . . . . . . . . . . . . . . . . . . . . . . . . .10
Lysing agent (green connector) . . . . . . . . . . . . . . . . . . . . . .10
Rinsing solution (blue connector) . . . . . . . . . . . . . . . . . . . . .10
Drain (red connector) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
<F8> SETUP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
<F1> Reference values . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
<F2> Date and time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
<F3> Result Printout. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
<F4> RS232 settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
<F5> Reagents and waste . . . . . . . . . . . . . . . . . . . . . . . . . .19
<F4> Other Set Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
Distributor SETUP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Prime . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Starting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
 1 General environment
Celly 70 should be installed on a clean and stable the factory). Reagent temperature is important for
benchtop, free of exposition to direct sunlight, good operation (do not store reagents in a cold
draft (draught) or dust. Celly 70 works at ambient area). Keep Celly 70 apart from all intense sources
Temperature (18 to 26°C). Reagents should be of electrical interferences like centrifuges,
stored between 8°C 6[46°F], and 30°C [86°F] and motors,...
used from 18° [64°F] to 26°[79°F]; note that White
Keep around Celly 70 at least 20 cm free to insure
Blood Cell Differential is compromised above 26°C
proper ventilation. Also take care to not obstruct air
[79°F] (electronic setting being adjusted according
circulation below instrument.
to ambient temperature : 20~22°C [68~72°F] at
☞ Attention Celly 70 must be operated only by qualified and trained operators. Take seriously blood sam-
ples handling. To avoid any accidental exposition to pathogen agents as HIV or hepatisis virus,
it is recommended to wear blouse, gloves and even safety mask and eyeglasses.

☞ Warning Never put any vial filled with liquid on the top of the instrument

☞ Warning To move instrument, place hands under chassis (not under front cover). To avoid backache,
maintain back straight and it is highly recommended to move instrument with two people.

☞ Important Hematology analysers are very sensitive to the quality of power supply. It is mandatory to con-
nect Celly 70 on a grounded plug and to UPS (Uninterrupted Power Supply) if stability of main
voltage is not satisfactory
 2 Celly 70 components

2.1 Fluidics

• Turret • WBC Metering Tube

• Dilution Cuvette assembly • Internal Vacuum/Pressure Reservoir
• Liquid syringes • Waste Pump
• Vacuum/Pressure syringe • Waste Detector
• Counting Chambers
2.2 Hardware

• 486 mother board • 2 serial ports RS232 to SIL and optionnal ther-
• Specific hematology electronic boards mic printer
• 6"1/4 color LCD TFT display • Parallel port for optional printer
2.3 Power supply

• 115 or 230 V (autocommutation), 200VA, 50 or • Fuses : 2 x 2A for 230 V, 2 x 4A for 115 V (fast)
60 Hz
☞ Attention If commutation from 115 to 230 V is automatic, control fuse value before this operation
 3 Unpacking
This picture shows how is installed Celly 70 in its
housing without external body. The first step is to
remove the top
 4 Instrument preparation
Remove protection films (right door, screen and
turret). For access to turret, remove front cover :

Pull the front cover

straight and progressive-
4.1 Access to fluidics

To have access to holding pins together

fluidics, open the
right plexiglass
door, then remove
the shield that
propects
chambers : it must
be removed
straight, the three

4.2 Control of fluidics

After open black door located on the right side and re-
all move all the clips from electovalves. Keep them in a
con- safe place, they can be usefull if instrument is
nec- stopped for long time. Check if no tube is bent and
tions, if all of them are correctly fit in valves. For help, re-
before fer to celly 70 fluidic schematics, page 73
switch
ing
ON,
4.3 Package

It is recommended to conserve original packaging

in case of transportation of instrument.
 5 Reagents1 and electric connections
30 centimeters of space
20 cm (8") can be saved by450using 5 liters
mm (17.7")

420 mm (16.5")

450 mm(17.7")
Container shown to
compare encombrance.
In such case, consump-
tion of HEMAREF II con-
siderably increases
)
2"
(3
m
0m
80

Keep space below instru-

Weight 25 kg

Installation with reagents on

the floor. Recommended.
For rinse solution, 5 liters con-
tainers as shown. Two
advantages :
- different size of con-
tainers -> decrease
risk of error
FIGURE 1. Installation Celly 70 (front) - lower consumption,

All connectors are located at the back of the analyzer :

1. For reagents reference codes, refer to reagents appendix
• Power cable All tubes, color coded, are located at the back of the
• Keyboard cable analyzer. Reagents containers can be installed on
• Printer cable (optional) parallel or serial the level benchtop or on the floor (recommended,
• RS232 Communication Cable (2, optional for refer to diagram). All Hycel reagents are compati-
LIS or DPU printer) ble with all Celly 70 parts.

DB 9 male (COM 2 : serial printer DPU or LIS)

DB 9 female (VGA CRT screen ; not used)
Not used
DB 25 (parallel printer)

DB 9 male (COM 1: LIS or serial printer DPU)

Keyboard
Diluent (white)
Fuse (2A for 230V, 4A for 115 V)
Rinse (blue)
Power switch

Lyse (green)
Power chord

Air syringe purge(yellow)

Waste (red)

Distance between back of

Celly 70 and wall must be
20 cm (8") or more
nt
Dilue

Rinse
Waste

FIGURE 2. Installation Celly 70 (back)

5.1 Diluent (white connector)

Tygon Tubing: 2 x 4mm diameters (3/32" x 5/32"), 70 analyzer. HEMATON PLUS must be used in or-
1.5m (4.9 feet) length der to produce a white blood cell differential analy-
sis.
HEMATON PLUS : azide-free isotonic solution for
diluting whole blood specimen with Celly
5.2 Lysing agent (green connector)

Tygon Tubing : 1.5 x 3mm diameters (1/16" x 1/8"),
0.50m (1.8 feet) length.
CELLYSE : it rapidly breaks down red blood cell
membrane, freeing hemoglobin and reducing the
size of cellular debris to a level that does not inter-
fere with white blood cell analysis. In addition, it
contains a cyanide radical that combines with the
free hemoglobin to form stable cyanmethemoglo-
☞ Warning Agitate bottle to remove water drops condensated on wall (especially in case of high tempera-
tures)
bin. Its absorbance is directly proportional to the
hemoglobin concentration over the clinically useful
range.
CELLYSE must be used in order to produce a
white blood cell differential analysis. This reagent
is stable for 30 days at room temperature after
opening.

5.3 Rinsing solution (blue connector)

Tygon Tubing: 2 x 4mm diameters (3/32" x 5/32"), counting cycle and provides rinse solution in the
1.5m (4.9 feet) length. aperture at the completion of the cycle and for shut-
down procedure. This rinsing solution is compati-
HEMAREF II is highly concentrated enzyme
ble with the plastic parts of the counter.
based, azide-free, isotonic cleaning detergent
solution. It is used in the metering tube during the
5.4 Drain (red connector)

Tygon Tubing: 2 x 4mm diameters (3/32" x 5/32"), Residues in the waste must be treated according to
1.5m (4.9 feet) length. local legislation. (Voir Cyanide waste elimination,
page A - 1)
Connect red marked tubing to fitting located on the
back of Celly 70. Screw the tap located at the other
side on the top of waste container.
 6 <F8> SETUP
Switch on the ON/OFF button at the back of the
analyzer, on the down left side.
Ver
Before doing anything, it is necessary to customize
instrument (laboratory header, printer and exter-
nal connection, reference values etc.)
Switch the printer on, if any. After program load-
ing, a first menu showing containers levels is dis-
played.
Press [ESC] key for access to MAIN MENU and
<F8> for access to settings.
To have access to SETUP, press <F8> in MAIN
MENU or move cursor with arrows keys to select
(highlight) SETUP menu and valid with <Enter>
key
6.1 <F1> Reference values

Reference values are already set. But it may be nec-

essary to adapt to local conditions. Also, each labo-
ratory should establish its own reference table.

Ver

☞ Tip Only one reference table is available. If necessary, it is possible to use veterinary mode to cre-
ate different «human species» (male, female, baby etc.)

Results out of these ranges are : - red in the results file (DATALOG)
- flagged with an arrow ↑ (high) or ↓ (low) in run
menu
 - printed in bold and underlined.

Ver
- Move the cursor with arrows keys or with - Edit new value and validate with <ENTER>
<ENTER> key. - Leave Edition with <ESC>
☞ Notes It is highly recommended to print references table
Value are displayed in International 1 system units
 <F1> let the access (with service password) to fine
settings :

Minimum
number of cells
MCHC is between
for WBC differential is
33 and 34
set at 1K/µL. Under
and a value >
this value, there is no
38 is impossible :
differential (not
system alert
enough cells for good
accuracy) Parameters
for interfer-
ences between
«small» RBC and
«big» Plts

L M G
(D2)

Ver WBC
(D3)

When there is a valley between low

(D4)
(D1)

and high threshold for mids cells,

the threshold is positionned at
50 100 200 300 400 the lowest point of the valley.
This adjustment can be modi-
fied a posteriori, «manually»
6.2 <F2> Date and time

Date and Time edition.

Format DD/MM or MM/DD depends of

setting (see date format, page 19)

Ver

6.3 <F3> Result Printout

Printout are in black and white. For color print-

outs the transfer of results with histograms to host
computer is necessary. In such case, management
of printout is done by LIS
Ver
• Epson LX and LQ type, Canon BJ 200, 210, 240,
250, 300 (Epson compatible)
Epson Stylus C80 or compatible
Hewlett Packard printers emulating PCL3, with
parallel port (HP 5650, 5652)
Serial thermic printer DPU 414, 40 columns
• 11" (28 cm) or 12" (30.5 cm) paper size (except
for thermic printer)
• Data are automatically1 printed after each run
or not.
1. It is not recommended to select automatic printout AND
automatic transfer in the same time (interest ?). This is
possible but as these proccesses are not fast, there is not
enough time to transfer data to 2 peripherials during dilution
cycle (30 sec.). If transfer is too long, it will be interrupted to
not delay next count.
• Reference ranges are printed or not
• With / without biologic alerts printout
• Half width / full width (except with DPU)
histograms are not printed, system and biologic
alerts are only printed with their abbreviation
(1 line per type of alert), after results
• With or without header : with header a space is
reserved for headed paper (except with DPU)
• Histograms can be printed filled (not available
with the DPU printer) or empty.2
2. When histograms are printed filled (in black), they are also
displayed filled (in colors). Note that this option empties ink
cartridges very fast and increases cost per test with no real
interest.
 • It is possible to select one printout (with histo-
grams) or two (without histograms) per page
(except with DPU).
• <F1> key (not displayed) : raw datas (for re-
search use). Thereafter raw datas are printed
on only one page. It is necessary to wait before
6.4 <F4> RS232 settings
Counts can be tranfered to host during count or
Ver
(and) from datalog.
running a new numeration ; so do not use this
option with automatic printout. This option is
not available with the thermic printer
• <F1> SETUP is displayed if a serial printer is se-
lected
Select highest speed possible with RTS/CTS
 Access to RS232 settings with laboratory
SETUP :
password («labo» by default)

Low transmis-
sion speeds are
not recom-
mended, espe-
cially when
histograms are
transfered.
Time to enter
identity during
count is de-
Ver

6.5 <F5> Reagents and waste

With laboratory password (labo by default)
For CELLYSE, 1 liter vi-
als should used only for
instruments perform-
ing more than 150
tests per day, else it
may perempted before
its end.
- For HEMATON PLUS and HEMAREF II 1:
• 4 numeric characters maximum, no decimal,
space or comma
• Maximum volume 20 liters
- For CELLYSE :
• 4 numeric characters maximum, no decimal,
space or comma
• Maximum volume 1000 mL
HEMAREF II
- For Waste

1. Correction factor is more important for HEMAREF II because

its flow depends of position of container, of pump, of age of
pump
 • 4 numeric characters maximum, no decimal,
space or comma
• Maximum volume 50 liters1
Corrections factors will be determined with the
observation of containers.
1. Remember that bigger is the waste, more it is difficult to
manage (heavy to move, decontamination)

6.6 <F4> Other Set Up

Access reserved by password : laboratory password

is <labo> by default. Other passwords and settings
are reserved for service.

Ver
 Ver
• Language selection : French, English, Spanish, • Date format
Italian1 selected with F10 DD/MM/YYYY
• Customizable laboratory name : two lines of 40 MM/DD/YYYY
characters each 2 characters for day and month, 4 for year
• Default mode selection: HUMAN or VET se- (from 1980 to 2099).
lected with F10 When format is not correct, an alarm window is
• System units selection :selected with F102 displayed reminding the right format. After
pressing <ENTER> key, previous date is dis-
played in an alarm window, with cursor at the
International 1 k / mm3 M / mm3 g / dL beginning of field.
• Delay before autorefilling : delay before refill-
SI 2 Giga / L Tera / L g/L
ing counting chambers from 10 to 60 minutes
2. it is recommended to select units once and for all to prevent
1. or other languages provided by local distributor wrong results in various unit systems
☞ Note it is better to use 10 minutes (it is only a refilling cycle to prevent apertures and glass pipes to
dry)

• Number of count sequences : select with key precise, especially for platelets and allow better
<F10> one or two sequences. By default one se- detection of partial clogs.
quence run to have the highest throughput (> 70 1 sequence -> speed
tests/hour). Two sequences counts are more 2 sequences -> quality
☞ Note For Start-Up and calibration procedures, always two counting sequences are performed inde-
pendantly from this setting

• Plt bias (Customizable, from 0 to 15) : this value • PDW correction factor : 4 numeric characters
is substracted from platelets counts. Use prefer- from 0.75 to 1.25. If 3 decimals are entered,
ably a low value (5 to 10) number is rounded with 2 decimals
• Predilution correction factor : 4 numeric char- • Laboratory password : maximum 22 α−nu-
acters from 0.75 to 1.25. If 3 decimals are en- meric characters. Small and capital letters are
tered, number is rounded with 2 decimals differenciated. By default <labo>
• RDW correction factor : 4 numeric characters
from 0.75 to 1.25. If 3 decimals are entered,
number is rounded with 2 decimals
☞ Note At the end of installation, it is recommended to print all setups (printer, serial, passwords etc.)
and to keep printouts in safe place (with computer setup for example)
6.7 Distributor SETUP

In <OTHER SETUP> menu, pressing <F1> gives

acces to distributor settings (with distributor
password) :

Limits of cell counts trigerring decontami-

«factory» calibration factors. When instrument is installed,
all laboratory calibration factors are set at 1.00
WBC time of flight reference has been determined in factory
and should not be changed. If atmospheric pressure is af-
fects TOF, modify altitude correction factor (service )
Logos may be changed (service)

Distributor password is «distri» by default. As this (<day of week> + <day of month> + <month>) x
password can be changed and lost, there is a uni- 123
versal service password : Exemple : thursday 16th december 2004 ->
(4 + 16 + 12) x 123 = 32 x 123 = 3936
 7 Prime
From MAIN MENU select prime or press <F6>
(priming). For the first time, it is recommended to

HEMAREF II

prime reagents one by one. With <F1>, prime
HEMATON PLUS. Control if liquid is correctly
aspirated from diluent container. With <F2> prime
CELLYSE. Control if lyse is well aspirated from lyse
bottle. With <F3>, prime HEMAREF II. Control if
liquid is well aspirated. For security, prime again
reagents all together with <F4>
If there is problem of aspiration, check if all the
tubes are properly fit in valves, if no tube is bent, if
all fittings are waterproof (properly tighten,
enough not too much)
 8 Starting
Press [ESC] key for access to MAIN MENU.

HEMAREF II

Select "Startup" menu by pressing <F1> twice 0.5, RBC < 0.3, PLT < 30 and 179<Hb chan-
Check that the results obtained are correct (WBC < nel<255) else validation is denied.
☞ Note If validation is possible but «zeros» too high, it is recommended to rerun startup with F10, else
accuracy of results may be affected (residues are substracted from measured values)

☞ TIP If startup validation is difficult, leave startup procedure and proceed to count with <F3>, with
choice «no startup cycle». Perform one or two tests with any blood (even one day old), then
with no tube in the turret (blank or diluent test). Most of time background noise is decreased
to about zero. Last step is to proceed to startup that should be OK

Calibration should be checked with blood controls Go to RUN menu (<F3>), insert a homogenized
before running samples (Voir <F5> Quality, blood tube into the turret slot and rotate manually
page 37). counter clockwise the turret full stop into the in-
strument. Once the specimen tube is in position
under the sampling needle, the turret is locked in
and the dilution process starts. As the blood is sam-
pled, red light is ON. The light remains red during
the cycle. As sampling is done, the turret rotates au-
tomatically back to the initial position and the tube
can be immediately removed. Other operations are
automatic. Put another specimen tube in the turret
slot, when the front green LED light is ON again,
Celly 70 is ready for next sample test by turning tur-
ret.
If any error occurs for count, mechanical or fluid-
ic, a flag or message is displayed on the screen.
 MAINTENANCE
Table of contents
Access to parts . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Cover removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Access to chambers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Weekly maintenance . . . . . . . . . . . . . . . . . . . . . . . 30
Preventive maintenance . . . . . . . . . . . . . . . . . . . . . 31
Aperture cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Turret cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Needle cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
Pump check up and cleaning . . . . . . . . . . . . . . . . . . . . . . . .38
Keyboard skin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Long time stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Turret Kit replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Hygiene . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
 9 Access to parts

9.1 Cover removal

Pull cover straight

9.2 Access to chambers

• Shield must be removed straight

• When it is reinstalled, be careful with
tubes and stirring motor connections
 10 Weekly maintenance
- Purge of air syringe : with pressure variations,
condensation of water may occur inside air
syringe, decreasing its effective volume (poor
vacuum, with TOF increasing, incomplete liquid
transfer from dilution cuvettes to counting
chambers).
Open yellow fitting located on the back of the
instrument (see Figure 3, “Installation Celly 70
(back)”, page 11) and let liquid drain by gravity.
A better drain can be done by pulling air syringe
up and down (from main menu, <F7/F1> : Ser-
☞ Veryim- Rinse tube must be connected to the front of nee-
portant dle as shown on the picture
vices/service, then <air syringe> or during back-
flush (<F4> clean aperture and open purge fit-
ting)
- Cleaning of bench below Celly 70 : air orifices are
located under instrument and some dust could
be aspirated, spoiling vacuum tubes and syringe.
- Cleaning of turret (See turret cleaning, page 34)
- Control of needle : needle must be straight and
clean and properly installed in its holder
 11 Preventive maintenance
11.1 Aperture cleaning
- Before calibration and in case of repeted clog
alerts.
- Always perform this operation in service menu
(in order to prevent any automatic refilling cycle)
- Prepare tissue paper to wipe any drop of liquid
- In service menu (See aperture : drain before dis-
mounting., page 47) drain counting chambers.
- Open the right door and remove counting cham-
bers protection shield (page 8).
Disconnect electrode plug
Disconnect tubes from chambers (do
not pull tubes but push them with nails)
- Follow schematics to dismount apertures (same
procedure for RBC and WBC)
For WBC, bent
pins must be in-
stalled in UP and
DOWN positions
as shown

Chamber with aper- Remove the two Pull aperture holder to the
ture holder bent pins right
FIGURE 3. Counting chambers aperture dismounting

- There is one o’ring per aperture to avoid leak- -Prepare pure

ages. Remove it carefully to avoid contact with bleach (36°
bleach. Make sure that spare o’rings are always Chl). Put the
avialble in stock apertures in
bleach solution
at least 15 min-
utes. Don’t let
o’rings and
electric connec-
tors in contact
with bleach,
they would be
Bleach solution
damaged. Two
vials are provided with opened covers in acces-
sory kit.
- Then rinse apertures with deionised water. Dry - Leave service menu by pressing <ESC>.
with lint free tissue, then reinstall the o’ring. - Run several counts on diluent to make sure that
- Reconnect tubes background counts are correct.
- Reinstall external aperture holders on counting
chambers. Be careful with o’ring. Push bent pins
in place . Replug electrode. Re-install the cover
shield, right side first : be careful ! do not pinch
tube(s).
11.2 Turret cleaning

Recommended every week for 70 numerations/

day.

• Remove the front cover (page 28)

• Select the test «TURRET DIS-
MOUNTING» in service menu
• Disconnect the three transfer tu-
bings (yellow, orange1 and red)
and spring

1. orange fitting is for RBC/Plt : tube is

specific (rubber tubing, reference
524100055).

•
Pull the turret up, do not bend needle.
 Clean inside and outside of the turret with absor- Clean turret axis. If necessary lubricate it slightly
bent paper moistened with cleaning and disinfec- (just a film)
tant liquid (no solvent such as alcohol, some water Clean the dilution cuvettes with a cotton tip moist-
with dishes detergent or HEMAREF II is quite con- ened with HEMAREF II or ID109 (strong deter-
venient). Clean also black plexiglass (no alcohol nor gent).
solvent)
☞ Caution Never put fingers in dilution cuvettes ! Else, it is required to clean them

• Reinstall turret taking care of electromagnet
plunger position.
• Reinstall fitting transfer tubings to appropriate
color fitting and spring plug.
• After complete installation run 2~3 samples
with bleach in place of sample and then few
counts on diluent

☞ Attention Do not dismount dilution and evacuation chambers, except if absolutly necessary : plactic is
very thin and fragile. If cuvette holder is dismounted, think to put some silicon grease on
screws before re-installation, to avoid oxydation or (an) deposit of salt
11.3 Needle cleaning
Cleaning the internal side of the needle
being delicate, it should be done only by
service staff :
- go to service menu to prevent auto-
matic rinsing
- remove front cover (page 8)
☞ Warning needle must be absolutely fully inserted, with rinse tube in the front, other-
wise the external flow of diluent cannot rinse the needle properly, and a large
contamination may occur.
☞ Attention
Needle must be clean.
Salt deposit as shown
here is acceptable. Clean
with tepid water
Needle must be absolutely straight ;
during the external rinsing, the liquid
must follow the needle from top to bot-
tom, else needle remains dirty. If neces-
sary, stripe needle with very thin sand
Needle
FIGURE 4.
cleaning with
nylon wire
- Remove needle from potence and dis-
connect tube from the top
- use the nylon wire provided in the
accessory kit, push it from top to bot-
tom.
- Reinstall needle, tube(s), front cover,
quit service and run background(s)
paper (800) from top to bottom (never in
other direction!). Then it must be soaked
to help liquid flow (put ID109 or liquid
soap on tissue paper and apply on nee-
dle), followed by blank cycles
11.4 Pump check up and cleaning
Recommended every 6 month.
Cleaning of draining pump :
Remove the front cover
Disconnect red labeled tubing located on the
right of sampling needle
In place of this tubing, connect white labeled sil-
icone of accessory kit (length 0.5m [1.6 foot], ex-
ternal Ø 6mm [1/4"])
Put the end of this tubing in a vial filled with 50
ml (about) of Hemaclean.
11.5 Keyboard skin
Regularly check keyboard skin. In case of tearing,
it is recommended to replace it.
11.6 Long time stop
It is not recommended to let an hematology instru-
ment idle for a long period. As tubes are all filled
with salty solutions, risks of crystallization are very
high, with as consequences tubes
clogged, sometime impossible to clean (lyse, evac-
uation of draining chambers).
To let the analyser idle over one week (or for
storage) :
Select the test <drain sink> in service menu. The
pump starts for 12s and hemaclean is aspirated
from vial. Repeat operation until vial is empty.
Do not use water else pump will stop after 2 sec-
onds
With 50 mL, vial should be almost empty in 2 se-
quences
Then disconnect silicone tubing and reconnect
original tubing1.
1. Repeat this cleaning if necessary. Then if aspiration is not
correct, pump must be renewed (complete pump or only
internal membranes, call distributor)
1. Drain reagents by priming on air :
- remove tubings from reagent containers
(HEMATON PLUS, HEMAREF II, CELLYSE)
- from main menu, press <F6>, prime «ALL
REAGENTS». Repeat operation twice.
2. Prime with water (deionised)
- place tubings in deionised water and prime
(«ALL REAGENTS»).
 - press <ESC> to return to main menu.
3. Counts on water (deionised)
- press <F3> and perform three counts on water.
- press <ESC> to go back to main menu.
4. Drain reagents by doing a prime on air :
- repeat step 1
- switch off the instrument
11.7 Turret Kit replacement
Reference G710070
This kit includes :
- Turret
- Cuvettes and cuvette holder
5. Reinstall springs on pinch valves1
in case some springs are lost , remove pinched
tube from front valve slot of the double valves (1,
2, 3, 4, 5, 9, 11 and 12) : tube will not be damaged
after long stop
1. Don’t forget to remove springs or to reinstall tube before
restarting
- Base
- Optical sensors (installed)
- Solenoid (installed)
11.7 .1 Removal Before all remove the complete fluidic module
of assembly

To remove turret base, remove

the three screws holding base

and loosen these two screws

 Disconnect all plugs (optical sensors, solenoid,
tubes), remove the belt.

To disconnect
optical sensors
connectors press
with a thin screw-
driver there

To reinstall assembly, there is no adjustment. Be

carefull with belt. Re-fit tie-raps on cables to get a
clean installation.
11.8 Hygiene

External covers of the instrument should be regu- • the printer buttons

larly dust off (using a wet duster) and decontami- All components of the analytical system should
nated with a disinfectant moist towel. be decontaminated by the laboratory staff as
Do not forget explained in the preceding paragraphs before
• the turret any service intervention.
• the keyboard skin
 TROUBLE SHOOTING
Table of contents
WBC counting time (WBC time of flight [tof]) . . . . . . 46
WBC tof too long. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
WBC tof too short . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
Fluidic troubles . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Drain trouble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Transfer trouble . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Checking valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
Reagent problems . . . . . . . . . . . . . . . . . . . . . . . . . 49
No lysing agent. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
No diluent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
No rinsing solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50
Inversion between reagents . . . . . . . . . . . . . . . . . . . . . . . . .50
Other . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
MCV too low . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
WBC differentiation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
WBC histogram width . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Hemoglobin high and WBC very high . . . . . . . . . . . . . . . . . . .52
High Plt count on diluent . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Plt results low . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
Poor repetivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
Plt results too low . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
RBC and Plt results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
 12 WBC counting time (WBC time of flight [tof])
TOF reference can be modified in Setup (only by
service). To determine TOF, with a machine per-
fectly clean (apertures), run counts on diluent.
There should be no variation on TOF (maximum
12.1 WBC tof too long
• Dirty aperture:
Protein building on apertures reduces the diam-
eter and flow rate. This is affecting particles
counts and sizing. Such troubles are exceptional
when daily maintenance is properly done.
• Clogged apertures :
Fibrin, crystals, micro-clot in blood can block
the apertures. Dirty apertures clog more fre-
quently. If the problem is persistent, check for
reagent cleanliness and proceed to a cleaning
procedure (aperture cleaning, page 31). Also,
check if aperture o’ring is well fit.
12.2 WBC tof too short
• Air leakage in aperture, counting fluidics or me-
tering tube.
• Salt deposit in the metering tube.
• Low solution level in counting chamber, lack of
diluent, incomplete transfer to counting cham-
0.1 second). Compare TOF measured to TOF in
setup and change this one if necessary.
Note that TOF is measured and set when automat-
ic calibration is performed.
Reference time should be around 8 seconds.
• Vacuum too low
Check for silicone tubing air leaks (V6, V8, V9,
V12, V11) control if tube in V5 is well pinched
Detergent HEMAREF II missing
Problem with air syringe : purge it (see access
to parts, page 28)
• Atmospheric pressure too low. A correction is
available. This event happens especially in high
altitude location.
bers. Check valves 4, 5, 6, 10 and 12 (see transfer
trouble, page 47).
• Lack of HEMAREF II. Check if present in meter-
ing tube.
• Lack of HEMATON PLUS
 13 Fluidic troubles
13.1 Drain trouble
Bad drain can be caused by :
• valves problems (see checking valves)
• pump failure because of salt deposit on mem-
brane (refer to pump check up and cleaning,
page 38 to solve problem)
• Draining detector failure :
- Select service menu.
13.2 Transfer trouble
Incomplete transfer to the counting chambers :
some liquid can flow out of the front of instrument.
• Check if counting chambers are tight : be sure
that o’rings are correctly fitted (1 per aperture)
• Check fluidic connections on turret. Avoid to
pinch the tubings.
• Check if there is no clog in outputs of dilution cu-
vettes.
• Check valves 4, 5, 12 and 11 (see reagent prob-
lems, page 49).
☞ Note Instrument OFF for more than a week, check transfer before starting in order to avoid over-
flows at start-up.
- Disconnect red labeled tubing located on the
right of sampling needle
- Connect a syringe filled with HEMATON PLUS
(at least 10cc) to the fitting connected to the
drain detector.
- Select fluidic detector in service menu and check
drain detector when pushing successively air and
HEMATON PLUS.
• Look for pinched or clogged tubings ; discon-
nect the tubing from valve V4 for WBC ; for
RBC, there is no valve between dilution cuvette
and counting chamber. Connect a syringe filled
with distilled water to the tubings : push and
pull water in tubings.
• Check vacuum/pressure syringe (see air sy-
ringe (vacuum/pressure), page 45) and purge it
(access to parts, page 28)
13.3 Checking valves
• Do all valves operate properly (see tests, page
46).
• Some tubings can be pinched in valves. Remove
them from valves one by one and roll them be-
tween fingers, then re-install them. Make sure
that tubings are not twisted.
• Check for silicone tubings damages ; replace
them with same size tubing (in accessory kit,
1.98 / 3.18 mm (1/8" / 1/12") for two-way valves
and 1.58 / 3.18 mm (1/8" / 1/16") for one–way
valves).
 14 Reagent problems

14.1 No lysing agent

Numeration of WBC very high, bad differentiation,

Hb high. See example hereafter

WBC histogram is
saturated (because
without lyse, RBC
are counted).

• Valve problem : check valve 3 activation
• Tubing out of reagent in the bottle
• No more reagent in bottle : replace it
14.2 No diluent
• Pinched or sticked feeding tubing if left idle too
long : check tubings in valve V3. Both tubings
are silicone 1.98 / 3.18 mm (1/8" / 1/12"). They
should not be bent nor sticked.

Lack of precision and even rising drift • Tubing out of the container
• Empty container : replace it • Check valves 1 and 2 (activation and pinched
tubes)
14.3 No rinsing solution
• Empty container : replace it
• Tubing out of the container
14.4 Inversion between reagents
If HEMATON PLUS and HEMAREF II are invert-
ed (or HEMAREF II everywhere) cell membranes
are affected by surfactants present in HEMAREF
II. Consequently :
• MCV is very low
• Check valves 8, 9 and 12 (activation and
pinched tubes)
• WBC differential is abnormal (only one «peak»
in Lym position)
• Hb is normal
• Most of blood controls are normal (in blood con-
trols as cells membranes are very strong they
resist more than patient cells)
 15 Other

15.1 MCV too low

• Check if the o’ring is well fit in RBC aperture as- • Make sure that veinous blood is sampled with
sembly. EDTA K3
• An ionic short-circuit may appear between RBC
and WBC : change silicone tubing in valves V7
and V10.
15.2 WBC differentiation

• WBC differential analysis should preferably be
done on fresh blood sample (more than one 1/2
hour, less than 4 hours) and well mixed (2-5
minutes but not over-mixed) and with an ambi-
ent temperature between 18 and 26°C (64 to
79°F). Also take care of reagent temperature :
in case of storage at a temperature very differ-
ent from laboratory temperature, let balance
minimum one day.
• The anti-coagulant should always be the same
(dilution ratio, anti-coagulant quality,…), pref-
erably EDTA K3.
☞ Caution with blood control, WBC differentiation may remain correct if HEMATON PLUS and HEMAREF II
are inverted
• WBC aperture slightly dirty (see aperture
cleaning, page 31)
• Inversion between HEMATON PLUS and HE-
MAREF II : this drags WBC histogram to left
and only one peak remains in Lympho position.
MCV is decreased
• Lysing agent is affected by too high tempera-
tures (>26°C) and its efficiency may decrease
after 2 weeks. For laboratories doing few tests
per day, 250 mL bottles are recommended.
15.3 WBC histogram width
WBC gain should be adjusted in order to have the
end of granulocytes distribution between 300 and
330 fl for normal human samples. The average of
lymphocytes peak is around 60 fl.
15.4 Hemoglobin high and WBC very high
Probably lack of lysing agent : open the right door
(black plexiglass), remove counting chamber shield
and look at the lyse reaction in the WBC chamber
(red, turbid solution should become translucid).
• Check presence of lyse in bottle. Replace if nec-
essary
• Improper mixing : check motor (page 46)
15.5 High Plt count on diluent
1. Container
Empty diluent container : replace it
Plunger out of container
2. Pollution :
• Pollution of dilution cuvettes : The best way to
get rid of the problem is to rub the inner sides of
the dilution cuvettes with a cotton tip impreg-
nated with Hemaclean or better with pure
ID109. ID109 is a strong cleaning solution for
decontamination you may use either diluted to
prime the Celly, or pure to impregnate a cotton
• Lysing agent is affected by too high tempera-
tures (>26°C) and its efficiency may decrease
after 2 weeks. For laboratories doing few tests
per day, 250 mL bottles are recommended.
• Sticked or clogged tubing : check tubings in
valve V3
• If necessary, replace the two silicone tubings
1.98 / 3.18 (1/8" / 1/12") (thin wall, do not use
tubings with thick wall in double way valve).
• In case of both high RBC and high Plts, check
correct inflow of Hematon Plus (no bubbles in
tubings).
tip and rub the dilution cuvettes. You may order
it : p/n : 571300170 Designation : ID109 clean-
ing solution. Do not pour the Hemaclean or
ID109 in the cuvette (NEVER POUR PURE
ID109 INSIDE THE CUVETTES), just rub the
empty cuvette inner sides with the cotton tip im-
pregnated. Then start directly a startup cycle.
No more than 3 consecutive startup cycles
should be required to obtain acceptable results.
• Bad rinsing of needle : refer to needle cleaning,
page 37
 • Too much grease around the diluent piston and/
or the sample piston : Disassemble the diluent
syringe and the sample syringe from the dilutor
assembly. Wipe with a soft tissue both the pis-
tons and the inside of the syringe body to elimi-
nate all visible grease. A very very thin film of
silicone (provided by Hycel) grease is sufficient
for efficient lubrification (not visible, just
enough to make the o-ring bright). Any excess
will drive to high Plt background counts.
• Pollution of the reagents : Replace both HEMA-
TON PLUS by new containers.
• Pollution inside the sampling needle : refer to
needle cleaning, page 37.
3. Electronic noise :
• Bad earth connection : Check the earth connec-
tion from the laboratory mains installation to
the main plug of the instrument. Do not forget to
check the power cord and eventual extension
cords.
☞ Note When important noise appears and disappears, usually it is a problem of electric contact
☞ Attention In any case after such adjustment it is mandatory to check with blood (control and patient)
validity of settings
• Noisy mains : Use an on line UPS with sinus
output. Be careful as some UPS are sometimes
more electrically noisy than the mains directly
from the wall plug.
• Bad shield : Verify that the counting chamber
shield is well inserted. Verify that the pre-am-
plifier board shield is well screwed. Using an
ohmmeter, check the ground continuity between
the pre-amplifier shield and the pre-amplifier
board ground map, then between the pre-am-
plifier board ground map and the chassis. Con-
trol also spring plug ()
• Incorrect electronic adjustments : Verify Plt off-
set, gain and threshold adjustments. A too high
offset, too high gain or too low threshold might
drive to high background Plt counts.
• Some electronic components of pre-amplifier
board are very sensible and can be damaged by
electric chocks. Contact service department
• Aperture o’ring damaged or dirty : a ionic short
-circuit in parallel to aperture generates high
level of noise (change it).
 ☞ Attention It is recommended to let only service people to do these settings

15.6 Plt results low

On human bloods : needle may be dirty, refer to

needle cleaning, page 37
15.7 Poor repetivity

On RBC/Plt (and WBC) • Control mixing procedure

• Insert properly needle in its holder • Bent sampling needle
• Clean needle inside and outside • Needle may be too high or tow low (see sam-
pling needle, page 46)
• Check diluent fluidics
15.8 Plt results too low

• Control mixing procedure • Be sure that sampling needle does not touch bot-
tom of tube/vial (see sampling needle, page 46).
15.9 RBC and Plt results

• Needle may be bent or position not proper • Control WBC cuve (position, cracked ?)
(height, position to remove drop...) refer to sam-
pling needle, page 46
 ANALOGIC ADJUSTMENTS (MEASURE)
Table of content
Measurement principles . . . . . . . . . . . . . . . . . . . . . 56
Problems of centering and recirculation . . . . . . . . . . . . . . . . . 56
What is what? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Synoptic of measure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Hb adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
HGB offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Hb channel setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Offset adjustment on RBC, WBC channels . . . . . . . . . 62
RBC offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Platelets offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
WBC offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Analog board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Counting thresholds adjustments . . . . . . . . . . . . . . . . . . . . . 64
Swirling back adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Analog gains adjustment . . . . . . . . . . . . . . . . . . . . 67
RBC gain adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
PLT gain adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
WBC gain adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Preamplifier schematic . . . . . . . . . . . . . . . . . . . . . . 69
Analog board schematic . . . . . . . . . . . . . . . . . . . . . 70
 16 Measurement principles

16.1 Problems of centering and recirculation

%
130
PIV
110
PIII

PII 80
PI
PI
60 PII 0.5%
E E
40

20 PIII
1
After going through the aperture,
-0.5 0 0.5 1 1.5 x/d
2
because of turbulences, cells go
(aa) 5 back near aperture, giving small
When cells are not PIV
10
long pulses (RI, RII, RIII) that
well centered in aper- 20
30 must be eliminated from sizing and
ture (PII, PIII, PIV), counting (anti recirculation). In
40
50
60

there is distortion of Celly 70, RBC reference electrode

70
80
5

12
0 2 05 0

11

pulses.
90

1
1
95 is long to aspirate sample close to
96

1 00
aperture and a «filter» rejects too
100

10

long pulses. For adjustment, refer

2

10
5

110

to swirling back adjustments, page

125

RIII
RII
64
RI

100 %
PI
80

60

40 RIII
(a)
Trigger
20 RII Level
RI t
(ab)
Pulses are very long
compared with PI
Fig 5 : Centering and recirculation
16.2 What is what?
Plt gain -Plt threshold removes noise from measure. Too high threshold
eliminates small platelets.
RBC gain
-RBC thresholds eliminates platelets and noise from RBC
count. In case of very low MCV (goat, sheep...), this
RBC pulse
threshold may be decreased (with interferences of
Plt on RBC count) else some RBC are rejected
Plt pulse
Amplitude

RBC threshold

Plt threshold Time

Noise

Fig 6 : Aspect of signal

On preamplifier, offset and gain can be adjusted : a d j u s tPLT
ment, reference material is
necessary : usually fresh blood control,
Increase of DC gain
• Offset : when input is at zero, output is at
with for RBC, value of MCV, for Plt value of
zero. The noise (in green on above dia- 0 10 20 30
fL

MPV. For WBC peak of Lymp gives a first

gram) is symmetric around the base line.
approach RBCbut final adjustment is done on
Adjustment with voltmeter.
patient bloods.
Increase of DC gain
• Gain : adjusts the amplitude of pulses, so
is responsible of measure of volumes. For 0 100 200 300
fL

WBC
Increase of DC gain
16.3 Synoptic of measure
Gain

Hb

Offset

Elimination of negative pulses WBC and Hb channels

use the same converter

From µP : choice Hb/WBC µP (Z80)

ADC
WBC

Pulse OK
Pulse

Elimination of negative pulses

WBC pulses
5V Anti recirculation
Low threshold µP (Z80)
Gain µP (Z80) Gain

ADC ADC
RBC + Plt

RBC Plt
Pulse OK Pulse OK
Offset Offset
Pulse Pulse

RBC pulses Plt pulses

5V Anti recirculation 5V Anti recirculation

Low threshold Low threshold

Fig 7 : Schematics of signal processing (analogic)

 17 Hb adjustments

17.1 HGB offset

Disconnect the Hb LED connector at the Pre-am- Connect a voltmeter according to the polarity and
plifier Board input (J2). adjust the offset voltage just upper 0.0 mV with the
Connect a special adaptor at the Hb output of the potentiometer indicated on the Pre-Amplifier
Pre-amplifier Board (black Cinch cable.). Board cover. Reconnect the Hb LED connector.

17.2 Hb channel setting

It enables the user to adjust the Hb reference tomatic rinsing has been done, the chambers are al-
channel if necessary. ready full).
Press <F4>. The instrument fills the counting Then, the Hb reference value is displayed.
chambers with diluent if they are empty. (If an au-
 If the Hb value is out of es when the temperature increases : about 1 chan-
range (180 to 254), the in- nel for 1˚C).
strument beeps continu- Once the adjustment is correct, leave the menu
GAIN PLT
ously every two seconds with the <ESC> key.
G
A and a message is displayed The new Hb reference value is automatically
OFFSET PLT I
N
until the reference is ad- memorized and the S8 alert message (if any) is not
OFFSET
WBC H
justed. set during next counts. ()
OFFSET RBC B
Adjust the hemoglobin Hb calibration is not affected by new Hb reference
GAIN WBC
gain potentiometer (on channel in the range 180 ~ 254
GAIN RBC
preamplifier board, near
OFFSET Hb the metering tube) to 1. As long as Hb reference channel is in 180~254 range, results
reach 235 - 2451 at 19 - are OK. Nevertheless, higher it is, better is resolution

RBC WBC
21˚C. This adjustment (precision). Experience in the field shows that between 235
must be done according to and 245, results are the best and there is enough margin for
RBC WBC

the ambient temperature small drift. But, in any case, it is highly recommended to
warm-up instrument during about 30 minutes in order to have
(the Hb reference decreas-
good stabilization of power supplies and temperature

☞ Important This adjustment has to be done only after cleaning WBC chamber. Normally there is no reason
to change adjustment

☞ Warning Avoid direct strong light interference (sun or light spot) when plexigass smoked door is open.
Install counting chamber shield

When adjustment is over, press <ESC> to quit and

go back to Service menu.
 Fig 8 : Preamplifier implantation

J5
GAIN PLT
Pot 9

B200272-ED3 G
Hycel
Pot 10

A
I
OFFSET PLT N
PLT 5 Gnd Gnd
4 Id
J6 RBC
WBC
3
2
D+
D-

HB
1 BUS Gnd OFFSET H
OFFSET RBC WBC G
B
Pot 5

Pot 4
Pot 8

Pot1

Pot7
GAIN RBC GAIN WBC
Pot2

OFFSET Hb

RBC WBC
RBC WBC

J2 J4 J3 J1
LED Hb RBC WBC Hb sensor
Carte pre-ampli
Preamplifier board
B200272
 18 Offset adjustment on RBC, WBC channels
For the following adjustment,
the ground must be connected
to the analog controller on the
GND test point. Any other
ground point may result in
wrong adjustment.
Some CINCH connectors are
connected to preamplifier out-
puts and are accessible on the
back of instrument. From top to bottom, Plt, RBC,
WBC and Hb. For RBC, WBC and Plt, values are 3
Plt times higher than on corresponding test points
(JP1 for RBC, JP5 for WBC and JP3 for Plt).
RBC
A connection kit is available, reference :
WBC
534 500 130, including 4 cables.
Hb
The adjustment screwdriver is included in starting
kit.

18.1 RBC offset

Connect an oscilloscope on test point JP1 (RBC

INPUT) on the Analog Board and test point GND.
Adjust the offset to 0,00 V with the best precision.
RBC offset potentiometer is located on the pre-am- GAIN PLT

plifier board. G
A
OFFSET PLT I
N
OFFSET
WBC H
OFFSET RBC B

GAIN RBC GAIN WBC

OFFSET Hb

RBC WBC
RBC WBC
18.2 Platelets offset

Connect an oscilloscope on test point JP3 (PLT Adjust the offset to 0,00
INPUT) on the Analog Board and test point GND or V with the best preci-
connect a Cinch cable equipped at the other end sion. PLT offset potenti-
with an oscilloscope connector on the first connec- GAIN PLT ometer is located on the
tor (from the top) at the back of the analog control- G pre-amplifier board.
A
ler. OFFSET PLT I
N
OFFSET
WBC H
OFFSET RBC B

GAIN RBC GAIN WBC

OFFSET Hb

RBC WBC
18.3 WBC offset

Connect an oscilloscope on test point JP5 (WBC tor (from the top) at the back of the analog control-
INPUT) on the Analog Board and test point GND or ler.
connect a Cinch cable equipped at the other end Adjust the offset to 0,00 V with the best precision.
with an oscilloscope connector on the third connec- WBC offset potentiometer is located on the pre-am-
plifier board.
☞ Note : Always check during a diluent count that the offsets are properly adjusted and that the back-
ground signals are stable and less than
- for Plt,120 mV peak-to-peak if connected at the back of the analog board
- for RBC, 60 mV peak-to-peak if connected at the back of the analog
- for WBC,50 ~ 60 mV peak-to-peak if connected at the back of the analog board
 19 Analog board
☞ Important For the thresholds and swirling back adjustments, the ground must be connected to the GND
test point on analog controller board. Any other ground point may result in wrong adjustments.

19.1 Counting thresholds adjustments

1. RBC : connect the + of a voltmeter on test point 3. WBC : connect the + of a voltmeter on test point
JP2 and adjust the voltage to 150 mV +/- 0.5 mV JP6 and adjust the voltage to 200 mV +/- 0.5mV
with potentiometer P1. with potentiometer P5.
2. Plt : connect the + of a voltmeter on test point
JP4 and adjust the voltage to 65 mV ±0.5 mV
with potentiometer P3.
19.2 Swirling back adjustments

☞ Note: These adjustments must be done with an oscilloscope (1 VDC, 10µS). It is advisable to perform
these adjustments during blood counts (e.g. normal control blood) as it is easier to visualize
the signal in such conditions.

1. RBC : connect an oscilloscope on test point JP8 3. WBC : connect an oscilloscope on test point
and adjust the pulse length to 50 µS with poten- JP10 and adjust the pulse length to 50 µS with
tiometer P2. potentiometer P6.
2. Plt : connect an oscilloscope on test point JP9
and adjust the pulse length to 48 µS with poten-
tiometer P4.
☞ Note See on next page test points on diagram
 Fig 9 : Analog board implantation
200 ± 0.5mV with P5 65 ± 0.5mV with P3
White (WBC input) Red (RBCinput)

Black (Hb input) WBC threshold Plt threshold Yellow (Plt input)

JP13
J5 J4 J3 J1 RBC threshold
U19 U23 U12 U4
U17 U10 U1 CA310 CA310
CA3100 CA3100
AD7575 AD7575 AD7575
+ RBC
JP5 U16 U3
WBC
JP4 antirecirculation
input JP6 TL071 CA310
Plt +
WBC threshold
U11 U2
threshold U18 P1 P2 JP1 RBC input
+ + + JP3
+ + + + + Plt
U13
input
JP7

U8 P3 P4 +
GND + + + U5
4066
U20
+ P5 P6

WBC antirecirculation Plt antirecirculation JP12 +

JP2 RBC threshold
U22
+
U9 U15
+
U15
150 ± 0.5mV
74LS132 4066 74LS132 74LS132
+ with P1
JP 10
50 µS with P6 WBC JP 9 JP 8
RBC
Bring Plt
Bring Bring 50 µS with P2
U21 U24 U14 U6

4538
+ 4066 4538 4538

48 µS with P4
 20 Analog gains adjustment
For the gains adjustments, all calibration factors Offsets, thresholds and anti-recirculation must be
must be set to 1 (see Calibration menu). A normal set before these adjustments.
blood control must be used, in control mode. Check Instrument must be perfectly clean (apertures, re-
its expiration date and make sure it was kept in agents, no background noise)
good conditions and properly mixed. Low or high Remember that when gain increases, histogram
controls are not recommended. drifts to the right.
20.1 RBC gain adjustment

Adjust the RBC gain in order to get the target MCV On human sample, RBC indices should be within
indicated for the normal blood on the Control As- normal ranges.
say Sheet.
20.2 PLT gain adjustment

Adjust the PLT gain in order to get the target MPV Run human samples to check the PLT histograms
indicated for the high control blood on the Control shape.
Assay Sheet. The usual mobile PLT high threshold A low PLT gain adjustment may result in high PLT
is close to 20 fL. Sometimes it's difficult to see it. On results as, in such case, small RBCs may be counted
human blood MPV is 9 fL. A partial blocking of the as PLTs.
orifice increases the MPV (MCV too, but less). On normal human samples, the average PLT high
Sometime the blocking is so low that MPV is the threshold is found between 20 and 30 fL.
only parameter affected.
☞ Caution Normally threshold is set in valley between Plt and RBC distributions. But if big platelets are
proportionally important, some rippling may appear on the right of Plt histogram and threshold
can be set in wrong position. This happens more with low Plt counts and on low controls. In
such case, use manual moving thresholds to correct software interpretation
20.3 WBC gain adjustment

1. With blood control : check that thresholds are in correct

right positions (choose control mode, not 2. With patient blood : when samples are normal
patient), especially between Lymphos and Mids. average of end of Grans should be between 310
End of Grans population should be between 350 and 320 fL, maximum 330. Use several blood
and 360 fL. No alarm should be displayed (D1 samples to have a good idea of adjustment.
means gain too low) and 3 part diff should be
☞ Caution: Always re-check offset adjustments after final gain adjustments as they may slightly have
changed.

L M G
(D2)

WBC
(D3)

(D4)
(D1)

50 100 200 300 400

End of Granulos popu-
lation on patient
blood : 320 to 330 fL
 21 Preamplifier schematic

J2 C42
U3 R6

V+
V+ +5V V+ VI VO 2 C2
R48

1 Hb LED
C41
U13 Com POT1 (gain Hb)
C4
U6
VI VO C5 D2 C3
R2
Com CA3100
C6 V+ V+
+24V C38 J1 V+ POT2 (offset Hb)

V-
U1 C43 D1
3 U2
V+ R20 R3
V+ 2 TL071 R5
R14 1 TL071
Hb
C11 POT4 (gain WBC) HB photo

Hb
R36 C13

R38
sensor
V- C46
R13 R16 C30 C1 R1
V-
R4
C28
R18 U7
C12 R15
U5 WBC
C14 TL071
3 2 Q2 D TL071 WBC +24V V-
G
WBC J3
4 5 C29 POT5 offset WBC)
C9 S C25 C17 C8
C31 R51 R49 R21 Molex 6pts
R17 R19

Gnd
R11 R12 C10 R39 C48 C15 6 5 4
R37
R45
D4 V+ C52 1 J5
C27 out U4 in 8
3 2 1
R55 R53 adj nc
C50 C16 C7
gnd nc
nc /shdn
V- R23
C54 LT1121_H R7
R52 R50 +24V R9
C40 V+
C26
V+

C39 D5 V+
+V C49 1 8
U10 R54 C53 out U8 in
D3 R56 R22
adj nc
CA3100 gnd nc
C44 C51 R8
-V nc /shdn
C55
+24 C45
R24 LT1121_H
V-

R10
V+ R34
V+
R28
POT7 (gain Plt) POT9 ((gain Plts)
C20 R35 PLT 5 Gnd Gnd
C22 J6
R42

R40
4 Id
C24 RBC 3 D+
2
+5V

C34 D-
1
R27 POT10 (offet Plt) WBC BUS Gnd
R30 C32 U12
R32 +V U11
C21 R29 U9 HB
RBC
C23 TL071 TL071
3 2 Q4 D TL071 -V
RBC G R47
J4 4 5 S C33
/Plt C18 POT8 (offset RBC) R44
V-

R33 C35
R31 R43 R45
R25 C19
R26 C36
R41

V- Carte pre-ampli
V- RBC Preamplifier board
B200272
 22 Analog board schematic

LM317 LH 16
AIN
1 3 CLK Z80A 5
CLK
Vin Vout 17 14 A PB0
VCC VREF 080
13 A PB1
3 081
+ VCC TP 12 A PB2
1 082
CS 11 A PB3
ADJ 2 083
RO 10 A PB4
084
085 8 A PB5 JP12
2 15 7 A PB6
AGND 086 RBC
087 6 A PB7 1
Plt 2
Busy 4 3
WBC 4
-12V VCC 5
JP1 6
Hb

+
VCC 7
8
RBC 9
4 8 1 4538 10
2 Pulses RBC
Jack J1 JP8 14 10
6 AC Q
3 15
P2 CX
CA3100 75 100KΩ 12 VCC
+T
11
4538 -T
9
VCC 2 6 5 A Q

+
AC Q
3
+12V 1 4
CX 13
4 AST1
JP2 +T 74LS132
LM78LO5ACZ 5 4066
3 1 -T 1 2
+12V Vin Vout 7
75 A Q
3
3
6 9
GND P1 2 8
13
+ + 10kΩ 10 9
CA3100 VCC 2
+ 8 1
2 4 8 1 VCC
10
+12V
74LS132 7416
-12V JP13
16 A P80
AIN 1
CLK Z80B 5 A P81
1 3 CLK 2
Vin Vout 17 14 B PB0 A P82
VCC VREF 080 3
13 B PB1 A P83
081 4
3 12 B PB2 A P84
+ VCC TP 082 5
1 11 B PB3 VCC A P85
ADJ CS 083 6
2 10 B PB4 A P86
RO 084 7
8 A P87
085 B PB5 8
15 CLK Z80A
2 AGND 086 7 B PB6 9
pulse RBC
087 6 B PB7 10
RST1
11
Busy 4 12
B P80 13
-12V VCC 14
B P81
B P82 15
+

VCC B P83 16
B P84 17
Platelets VCC B P85 18
4 8 1 4538 19
2 Pulses Plt B P86
Jack J3 JP9 14 10 B P87 20
6 AC Q 21
3 CLK Z80B
15 pulse Plt 22
P4 CX
RST2 23
CA3100 75 100KΩ 24
12 VCC
+T 25
11
4538 -T 26
9 C P80
VCC 2 6 2 A Q 27
+

AC Q C P81
3 C P82 28
+12V 1 1 C P83 29
CX 13
C P84 30
4 74LS132 C P85 31
+T 32
LM78LO5ACZ 5 4066 C P86
3 1 -T 10 33
7 11 C P87
+12V Vin Vout A Q 34
CLK Z80C
3 35
3 pulse WBC
9 RST3 36
P3 6 8
GND 2 START 37
+ + 10kΩ 10 4 12 38
TL 071 4 COM
+ 6 3 39
2 45 VCC VCC
5 40
+12V 41
74LS132 7416 42
+12V 43
-12V 44
-12V 45
16 46
1 3 AIN
CLK Z80B 5 47
Vin Vout CLK
VCC 17 14 B PB0 JP7 48
VREF 080
13 B PB1 49
+ 3 081
VCC TP 12 B PB2 VCC 50
1 082
ADJ CS 11 B PB3
2 083
RO 10 B PB4
084
085 8 B PB5
2 15 7 B PB6
AGND 086
087 6 B PB7
Hemoglobin

Busy 4
Jack J5 4066 4066
8 9 2 1

VCC

13
6
+12V

+12V
-12V VCC
+

VCC

WBC VDD V+
4 8 1 VCC 4538
Jack J4 2 JP10 Pulses WBC
14 10
6 AC Q
3 15
P6 CX + +
Vin
CA3100 75 100KΩ 12 VCC
+T
11
4538 -T
9
VCC 2 6 5 A Q
AC Q
6
+12V 1 4
CX 13
4066
4 74LS132 4 3
+T
LM78LO5ACZ 5
3 1 -T
+12V Vout 7
A Q
3 7 5
3
6 9
GND P3 2 8 5
+ + 10kΩ 10 4
+ CA3100 3 4
2 4 8 1 VCC 6
5 +12V

74LS132 7416
-12V
FLUIDICS INSTALLATION
23 Celly 70 fluidic components
Tube N° Ø tube length (mm) material Tube N° Ø tube length (mm) material

1 1.98 / 3.18 105 Silastic 21 1.98 / 3.18 215 Silastic

2 1.98 / 3.18 290 Silastic 22 1.98 / 3.18 50 Silastic
3 1.98 / 3.18 70 Silastic 23 1/16 - 1/8 205 Tygon
4 1.98 / 3.18 70 Silastic 24 1/16 - 1/8 30 Tygon
5 1/16 - 1/8 15 Tygon 25 1.57 / 3.18 85 Silastic
6 1.98 / 3.18 125 Silastic 26 1.57 / 3.18 60 Silastic
7 1.98 / 3.18 130 Silastic 27 1.57 / 3.18 25 Silastic
8 1.98 / 3.18 60 Silastic 28 1.98 / 3.18 165 Silastic
9 1.98 / 3.18 60 Silastic 29 1/16 - 1/8 15 Tygon
10 1.57 / 3.18 50 Silastic 30 1.98 / 3.18 285 Silastic
11 1/16 - 1/8 15 Tygon 31 1.57 / 3.18 110 Silastic
12 1/16 - 1/8 20 Tygon 32 1.98 / 3.18 180 Silastic
13 1/16 - 1/8 80 Tygon 33 1/16 - 1/8 105 Tygon
14 1/16 - 1/8 85 Tygon 34 1/16 - 1/8 55 Tygon
15 1.98 / 3.18 170 Silastic 35 1/16 - 1/8 15 Tygon
16 1.98 / 3.18 235 Silastic 36 1/16 - 1/8 60 Tygon
17 1.98 / 3.18 155 Silastic 37 1/32 - 3/32 25 Tygon
18 1/16 - 1/8 300 Tygon 38 1/16 - 1/8 255 Tygon
19 1/8 - 1/4 500 Tygon 39 1/16 - 1/8 65 Pharmed
Tube N° Ø tube length (mm) material Tube N° Ø tube length (mm) material

20 1/16 - 1/8 305 Tygon 40 1/16 - 1/8 85 Tygon

 24 Celly 70 fluidic schematics
Sample syringe
Lyse syringe
Diluent syringe Seringue échantillon Needle rinse Seringue lyse
Seringue diluant Rinçage aiguille
Lyse input
Arrivée lyse
Tubes filled with grey are
located in valves front slot 10
9 V5 V6
37
Les tuyaux remplis en gris
sont positionnés dans la fente 8 11
1 2 3 4
avant des vannes 12
7
V2 14 V4
V1 V3

tube volumétrique
4 13

Metering tube
40 15
3
1
5 6 38

From WBC cuvette

18 RBC chamber WBC chamber
De cuvette blancs Chambre GR Chambre GB
From RBC cuvette
39
De cuvette rouge 17
From sink cuvette
De poubelle
20
16
29 36
2
32 30
27 30
28
25 21
31

réservoir pression / vide

V7 V9 V11

Vacuum / pressure jar

V8 V10 V12

22
26
23
24
19 33 32
A
34
IR

Dr
a
dé in d
tec ete
teu cto
r li r
ge

qu
ide
rin

Detergent input sy
Arrivée detergent A ir
Pump
pompe
Diluent input
Arrivée diluant
 25 Celly 70 fluidic diagram

Diluent Single valve closed

Sample Lyse
Double valve

Liquid syringes

Lyse input
1 2 9 green
yellow
18 8 V6
V5 11
4
4 12
Sampling
needle
V1 3 V2 V3 V4
7 14 13

metering tube
5 15 start
15 6
RBC WBC Air syringe
count 17 count
stop
29
WBC

16
RBC
Sink

32 30
yellow
31 28 Air syringe purge
30

Vacuum / pressure
orange 25 27

28 Waste pump

reservoir
Atmosphere

21

19 V7 V8 V9 V10 V11 22 V12

23

detector
2 26
drain
red
16 Waste red
33 34

Detergent blue

Diluent white