BD RE ME: eS HH Kor. J. Vet. Publ. Hith, Vol. 8 No. 1 SSK FACE AWB At WF 2, AMBRE HBS HE] AMES! AMS! TM MME By WR AHtAF(R. plasmid) BARE RMN EOE & ES MEAL ABH RAK EL om = Abstract = Studies on Pathogenic Escherichia coli Isolated from Chicken with Colibacillosis 2. Antimicrobial Drug Resistance and Transferable R Plasmid in Escherichia coli Ki Seuk Kim, D.V.M., M.S., Ph.D. Institute of Veterinary Research, Office of Rural Development Rhun Bin Tak, D.V-M., M.S., Ph.D. Department of Veterinary Medicine, Cotlege of Agriculture, Kyunpook National University Three hundred and ninety-one strains of Escherichia coli isolated from chicken with colibacillosis were tested for their resistance to 13 antimicrobial drugs by the use of disc diffusion test. The transfer of drug resistance by conjugation was also tested, Ninety percent of the 391 strains tested were resistant to tetracycline, 82.9% to streptomycin, 80.3% sulfonamides, 68.3% to nitrofurantoin, 44.5% to carbenicillin, 32.5% to ampicillin, and 30.2 % to chloramphenicol, while percent of strains resistant to neomycin, kanamycin, cephalothin and trimethoprim-sulfamethoxazole was less than 30%, respectively, and 1 strain was resistant to gen- tamicin, and all of the strains were susceptible to colistin. Three hundred and eighty five strains(98.5%) of 391 were resistant to one or more drugs tested. Fifty five(61.1%) of 90 strains which were resistant to one or more drugs transferred all or a part of their resistance patterns to the recipient strain of F. coli ML 1410. Alll of five strains re- sistant to 6 drugs transferred the resistance, and the frequency of resistance transfer increased, as the number of resistant drugs increase. Poe BE MADRS) eco s FAL vb atch * * AG. 2e1G o]& RMS) EM He Ke ‘Kel DA A Ml OA THEM) HR ‘Kt DL KRA Loy AMM AAA & € RS] KK Mt MAS WLS Oe RHE PES] RO TAREE ASS Ofer} Simi AS RM AM ae Se — ee Al THE BMI7E Le] GASIo] Skool ee fH HRS) TRERS Asl7a lod dese MS 8) ol ARIS] RETIN HLS Hea = o1S MES) MLS HR SAL RRADIL HE MN HES] let rammcnusauazeansn, Rea] AMO] AEA HES 2& MPLAB sloldt R plasmid 7H) site HS MHC] Kop Veo} ope}a} clo Ve HIRT AS s}o} Bepernamoniensy, gahpstop AE ARMA oy 29, sf xp” gh yew 2.2 Ie] AMER MPAA 91614 R. plasmids) ise) A eR Ub See, ee AIRE oh RES) TOR BE MSY TM AMM Nol) BH HL Hol AL GRIES ItHIS!_R. plasmids 39 EHS 75} ae Wield. Se] HSS AMM ASE HMM ANS] SA CE Rl Ae BRIER Bae sie} THES) AROUNE SH MITEMEATS: HeAHEOh at HERS Slot RITES] R. plasmid (ATES AAAAG MOS MS Hele wel, BAL Be seit kA ARI BS He] ME lle aBBHRS 7} SUC WMS He] OREM Ae WHO 301 ee geatet sich. MIE MEM Disc A SL Ree Mo HE c| Aa ampi- cilling 138419) 2 SS} MIRA ROE 1 BA a. eR Bryant®s| Jikel ##bsl2o} Brain Heart In- fusion Broth(BHIB)= 4} 37C 2 18~24850M iii JEM RS ALAR MVEHE(O.36N H,SO. 99. 5ml 3} 0,04M Bacl: 0. 5ml# Efrat} Hz) 2 SEE NG GS AS ol MS iAAlae}7t Muller Hinton(MH) ARAL $2] APL Sensi-disc dispenser Ae} Hi MMNdiscE FHL THA Hla ee 37C ol AA 18~ 24860 SEMA ES AAA] dicsF shal) Hee 2 BT LEM) GES Meso wt AE each ea Hitt AMM Se disciéal Je) 1 MLE aM of tke] We FMMS ORE Hho AHS SE KR MMS Hel a nalidixic acid(Na)ol YES) Escherihcia coli M1410 (Fi Table 1. Antimicrcbial discs used for durg susceptibility test Disc potency Ampicillin (Ap) Tr Carbenicillin (Cb) 50 ne Cephalothin (CA) 30 ne Colistin (CI) 10 ne. Chloramphenicol (Cm) 30 ue Nitrofurantoin (Fm) 300 we Gentamicin (Gm) 10 pg Kanamycin (Km) 30 us Neomycin (Ne) 30 ug Streptomycin (Sm) ys Sulfonamides (Su) 250 ns * Tetracycline (Te) 20 ng Trimethoprim + 125 pat sulfamethoxazole (Sx) 78 og, ‘AaLh (RAMLZE Ap (Beecham Research L- ab), Cm (Park Davis & Co.), Km(US.P. Inc.), Na (Stering-Winthrop Lab.), Sm (H&B AS), Su(A HARM) Te(Pfizer Cos 7H MANE ALFA St 22} 0] MME MacLowry¢" 3 Wolf: 9) Hike wpe} AT MS BA) ach, 2S 01 MBM A RMS) ITED 7 tho} = IRIES) MAI25ug/m11 Ap, Cm, Km, Na, Sm, Te ££ 100yg/lo} Sue BAR MHEKPKE 44 VES BHIBY 370= 1SHFRN SEMEL LS MM EMEA A 100K 8141 ‘@ Hi Steer$"2) multiple inoculator# °)& MAVEN $RMO}-D 374 18~ 24K HR HS MBL AES HS MESS Hee ste. CES TEMS atROL oe Cea MRE & %% Smls} BHIBo +} 18~24n6 0H 383A! THR O.lml& 441 %#% Smls) BHIBSl #Hibe} 37C 2 3~ 405M 7HE Hebel] Rae hs RL WHS MEER 1: 48) Hee Hee eto} Lemp S22 Na(25eg/ m3} Ape5vg/ml), Cme25ug/ ml), Km(25zg/ml), Sm(25vg/ml), Su(100#g/ml) = = TeQSug/m)& SA ee Hho MAsto} 37C AA 18~24RGI) HSER 8 HERR) fi oF TEMES HZ abate. R SR KMS Ae TATE MMO ae UT A