CHEMISTRY 333 Principles of Biochemistry Spring 2012 Third Exam April 11, 2012 wwe, KEY _120 points JS points 3 points 10 points 6 points 196 points £2 points (Extra Credit) TOTAL: 100 points,4. Matching: Match the terms with as many terms in the alphabetized list as are applicable. Some may be used more than once and others not at all. There will be 20 total answers. Do not put more than 20 answers — there will be a deduction for each extra answer. Wrong answers will not count against you. (20 points) Tacyaiyoros A, Dy F Phospholipids F , @ ‘Sphingomyelins Peripheral proteins _( , 44, Cholesterol oar eat eee Giycogen Non-potar lipids ‘Transporters or channels [Noripids that exist as parts of or associated with biological membranes: Found stored in adipocytes Look ike steroids Contain a glycerol backbone Polar lipids Easy to remove trom membranes with salt or change in pH Glucose storage in animal cells Insulate nerve axons Can both increase and decrease membrane fluidity Has been reported to cause anal leakage Lipids found in biological membranes EC AL -zOnmoogm> 2 Draw and label a simple cartoonidiagram to represent a portion of a lipid bilayer membrane. Label the hydrophobic portion of the membrane and the hydrophilic portion of the membrane. (5 points) wpe BR omen (toil s)3. Match the molecule below with the class to which it belongs. (One answer per line) (10 points) A. chotestrat__B ®. phosphotipiss_D ©. sphingotipia_E ©. triacyotyeorot_A unsaturated tty acia_C F. Give the NUMERIC SYSTEMATIC name of molecule 1S: Bhtians below (3 points) a H.e—0-C—(CH,),.cH, 9 HO-0-C—(CH),CH=CH(CH,),CH, H6-0-6—(cr,)‘4 Glucose-6-phosphate is formed from glucose in an ATP-utlizing reaction. (10 points) Using the following information, calculate the overall AG" for this reaction Glucose + PO $ Glucose-6-phosphate + H:O0 AG° = +11.6 ki/mol ATP+H:O 5 ADP +PQ, 4 AG? =-32.5 k/mol 2-20.48 wl 2. toute overt reaction Sluase + ATP bE lveose lo- phosphate + HD ©. _Inwhich direction wil the reaction proceed? Circle ONE, @. Given your answer in (a), s the overall reaction spontaneous or not spontaneous? Circle ONE5. Answer the following questions about avcolsi, (16 points) * fanctons where ANP a eyed Pyrwvote Kinase a ae Prophoglyterat Linas? vo iii anne ae. reactions where ATP ie consumed. neo exo nase on ee Prusprofructoici nase i. © Circle the arrows ofthe step(s) where NADH is mage, 4, Puta box(es) around the arrows that are regulated steps) €. What s the NET yield of ATP mace anaerobi slucose to pyruvate. (Do not include those ger 72 f. Suppose you discover a mutant yeast whose glycolysis pathway is shorter because of the presence of a new enzyme that catalyzes the reaction: ly in the conversion of 1 (mole) of rated from NADH). Give a number as your ‘alyceraldehyde 3-phosphate + H,0 + NAD" > 3-phosphoglycerate + NADH + H” ‘What isthe NET yield of ATP made anaerobically in this mutant yeast in the conversion of 1 (mole) glucose to pyruvate by this shortened pathway? (Do not include those generated from NADH) Give a number as your answer. D0 ATPMultiple Choice: (3 points each ~ 36 points) 1. Which one of the following statements about cellular membranes is FALSE? 8. Membranes are composed of lpids and proteins, © ‘Only lipids are moving within the membrane and all the proteins are anchored and immobile, Membranes are asymmetric in structure, © steam ny berger wren entre bah nap eid Se ares '@. Membrane proteins are classified as integral or peripheral, depending upon their ‘membrane location, 2. Which of thefllowing membranes would be mos uid? 2. one contain Ins wih ojunsaturated 18 carbontaty ac. © one contain ots wh polyunsaturated 18 carbon tty ae HF one consign with stated 18 carbontaty ae. 4. one contain oie wih surat 16 caborfaty acs €. Aloft above would have the same ity 3. The reaction to the right is an example of a(n) reaction: rape b. Oxtator-Recucton © © tsomerzaon ond fron ug enerayof ATP £ iene 4. What s the main difference between active transport and facilitated transport? 2. Facilitated transport uses proteins, but active transport does not. (B) Active transport uses ATP to power transpot, but fcitated transport does not. BS Acive vaneport occurs across the plasma membrane, bi tacltated vansport does nat 4. Active transport moves a substance from a region of higher concentration to @ region cf lower concentration. Faciitated transport moves a substance from a region of lower Concentration toa region of higher concentration, ©. Allof the above are differences between active and facilitated transport5, The reaction to the right is an example of a(n) roactl a. Hydrolysis . Onidation-Recuction 1 Per ¢ Isomerization eT E a wonstomaton ving “i ATP Pd Q crn ante 6. fan unfavorable biochemical reaction is coupled to ATP hydrolysis, the reaction will probably be a. stopped © riven forwara BF reverses 4. non-spontaneous €. not affected The bonds linking the final two phosphate residues in ATP contain a lot of ray because they are fe 2 hysoaen nse b sete bonds Dc eterbonas @ vvowstortse bars ©. yan bere 8, Seven of the ten reactions in the glycolytic pathway have free energy (AG) values close to zero. What does this tell us about those reactions? ‘They are near equilorium reactions. Irae leeetdanetet for pathway regulation. They are reversible reactions @ Alottne above. None of the above.8. A dehydrogenase is an enzyme that catalyz ‘a. a phosphorylation reaction ‘an oxidation-reduction (redox) reaction © Berelease of proton rom an aed a hydrolysis reaction an isomerization reaction 10, Elevated loves ofthe hormone stimulate release of gluco a. insuin >. estrogen © © evineprine @ ergosterol «testosterone from glycogen. 11. Allof the following are TRUE about glycolysis except it, 2. occurs without oxygen. . ends with formation of pyruvate, DG: involesredetion of NAD" degrades glucose to CO: and H0. © allofthe above are true 12, It glycogenolysis occurs to supply anaerobic glycolysis within a cel ATP made from a glucose molecule roleased from glycogen is a. 1 ATPIglucose b. 2ATPsiglucose C © sarPsigucose © 4 ATPsiglucose @. SATPsiglucose the net 7. Extra Credit: (2 points) Whois the author of your textbook “Essen Biochemisty” a Vet Voot b Boyer 4 siner @ Prats comely © Horen S