ASSAYOFALGINICACIDIN ANTACIDBYPOTENTIOMETRY

PROCEDUREFORPREPARINGSOLUTIONS:

Pr
epar
ati
ono
fst
andar
dso
lut
ion
:

Accur
atel
ywe i
gh250 mgo falgi
nicaci
dWRS an dt rans
ferintoa
250mlc oni
calfl
akandadd30mlo f10% HClan d60mlo fAc et
one.shake
thesolu
tionfor30minutes
.Th enfi
lterthesolu
tionusi
ngwh atmanfil
ter
paper42b yanl ar
gediameterBuch
nerf u
nnel.Thenwasht hefil
tercake
withwat e
rt oformthef r
eeacid. Thent r
ansferthefil
terpape rtoa
coni
calfl
askandadd50mlo f0.1
M NaOH.s hakeitfor15mi nut
es.Then
ti
tratetheexce
ssofNaOHwi th0.1M HClbypo tent
iomet
ric
all
y.

Pr
epar
ati
ono
fsampl
eso
lut
ion
:

Accuratel
ywe i
ght hesampleantaci
dsolut
ionequi
val
entto 250mgo f
al
gini
cac idina250mlc oni
calf
lakan dadd30mlo f10% HClan d60ml
ofAc e
ton e.Shaket h
es olut
ionfor30mi nute
s. Thenfil
terthes olut
ion
usi
ngwh atmanf il
terpaper42byanl arg
ediame t
erBuchnerfun n
el.Then
washthef ilt
ercakewi thwatertof or
mt hefreeaci
d. The
nt ransferthe
fi
lt
erpape rtoac onic
alf l
askandadd50mlo f0.1M NaOH.s h akeitfor
15 minutes.Th ent it
ratet hee xce
sso f NaOH wi th 0.1
M HClby
pote
ntiometric
all
y.

And al
so perf
orm t
he bl
ankt
itr
ati
on by t
hes
ame pr
oce
dur
e as
di
scus
sedabov
e .

OBSERVATIONS:

Lab
elc
lai
m:e
ach1
5mlc
ont
ain
s0.
200go
fal
gin
icac
id

We
igh
tofs
tan
dar
dal
gin
icac
idt
ake
n(Sw)=0.
25052g

We
igh
tofs
ampl
eso
lut
iont
ake
n(Tw)=20.
04579g

Ti
trat
ionv
alu
efo
rst
andar
dso
lut
ion=36.
4ml

Ti
trat
ionv
alu
efo
rsampl
eso
lut
ion=35.
7ml

Ti
trat
ionv
alu
efo
rbl
ank=48ml
Ne
tti
trat
ionv
alu
efo
rst
andar
dVs
=bl
ank–s
tan
dar
d

=48-36 =1
2ml

Ne
tti
trat
ionv
alu
efo
rsampl
eVt=bl
ank–s
ampl
e

=48-35.
7=1
2.3ml

Wt/mlo
fsampl
eso
lut
ion=1
.0723g
/ml

AMOUNTOFALGINICACIDPRESENTIN 1
5MLOFSAMPLE:

Vt Sw
= * *15*Wt
/ml
Vs TW

12.
3 0.25052
= * *15*1.
0702
12 20.04579

=0.
20563g

Re
sul
t:

Th
eeac
h15mlo
fsampl
eco
ntai
ns0.
20563go
fal
gin
icac
id.

Cau
tio
n:

Thefil
trati
ono fthebo ths tandardands amplesoluti
onsar emo r
e
i
mpo r
tantstepsinthet i
trati
on. Thewas hi
ngo fthefil
tercakewitht h
e
waterismandatoryforobtai
ningbetterres
ult
s .Wi th
outthissteptheHCl
aci
dwh ic
hisu sedforthes eparati
ono ftheal gi
nicaci
df ro
mt h
es ampl
e
mayc l
ogint hefi
lte
rpape r.Andwi thoutwas hi
n gtheadditi
ono fNaOH
re
actin
gwi t
hbo tho ftheal gi
nican dHClac ids. Sot hiswillshowt h
e
decr
easeintheendpo i
ntoft hetit
rati
onandf alseresul
tsalso.
 POTENTIOMETRICASSAYOFALGINICACIDIN ANTACID

VOLUMEOF
SL.
NO 0.1
MHCL STANDARD SAMPLE BLANK
CONSUMED

1 1ml -324.4 -318.

1 -333.9
2 2ml -322.1 -315.
8 -331.
6
3 3ml -321.
8 -315.
5 -331.
3
4 4ml -319.
4 -313.
1 -328.9
5 5ml -316.
9 -310.
6 -326.4
6 6ml -313.
2 -306.
9 -322.7
7 7ml -311.
5 -305.
2 -321
8 8ml -309.4 -303.1 -318.
9
9 9ml -307.5 -301.
2 -317
10 10ml -305.6 -299.
3 -315.1
11 11ml -303.1 -296.
8 -312.
6
12 12ml -301.
4 -295.1 -310.
9
13 13ml -300.1 -293.
8 -309.6
14 14ml -298 -291.
7 -307.5
15 15ml -297.3 -291 -306.8
16 16ml -296.6 -290.
3 -306.1
17 17ml -294 -287.
7 -303.5
18 18ml -292.4 -286.1 -301.
9
19 19ml -290.8 -284.
5 -300.3
20 20ml -288.4 -282.1 -297.9
21 21ml -285.3 -279 -294.8
22 22ml -282.9 -276.
6 -292.4
23 23ml -280.3 -274 -289.8
24 24ml -278.4 -272.1 -287.9
25 25ml -274.6 -268.
3 -284.1
26 26ml -271.
2 -264.
9 -280.7
27 27ml -268.3 -262 -277.8
28 28ml -264.6 -258.
3 -274.1
29 29ml -260.7 -254.
4 -270.2
30 30ml -255.9 -249.
6 -265.4
31 30.1ml -254.8 -248.
5 -264.3
32 30.2ml -253.4 -247.1 -262.9
33 30.3ml -252.1 -245.
8 -261.
6
34 30.4ml -251.
6 -245.
3 -261.1
35 30.5ml -250.8 -244.
5 -260.3
36 30.6ml -249.1 -242.
8 -258.6
37 30.7ml -247.7 -241.
4 -257.2
38 30.8ml -246.5 -240.
2 -256
39 30.
9ml -245.1 -238.8 -254. 6
40 31.
0ml -243.8 -237.5 -253. 3
41 31.
1ml -241.
5 -235.2 -251
42 31.
2ml -239.9 -233.6 -249. 4
43 31.
3ml -238.1 -231.
8 -247. 6
44 31.
4ml -236.4 -230.1 -245. 9
45 31.
5ml -233.2 -226.9 -242. 7
46 31.
6ml -230.4 -224.1 -239. 9
47 31.
7ml -228.6 -222.3 -238. 1
48 31.
8ml -225.4 -219.1 -234. 9
49 31.
9ml -223.8 -217.
5 -233. 3
50 32.
0ml -220.4 -214.1 -229. 9
51 32.1ml -218.
5 -212.
2 -228
52 32.
2ml -216.
3 -210 -225. 8
53 32.
3ml -213.
9 -207.6 -223. 4
54 32.
4ml -211.
3 -205 -220. 8
55 32.
5ml -210.
6 -204.3 -220. 1
56 32.
6ml -208.5 -202.2 -218
57 32.
7ml -205 -198.
7 -21 4.
5
58 32.
8ml -202.6 -196.
3 -21 2.1
59 32.
9ml -198.
7 -192.
4 -208. 2
60 33.
0ml -195.
3 -189 -204. 8
61 33.1ml -192.
9 -186.
6 -202. 4
62 33.
2ml -188.
4 -182.1 -1 97.
9
63 33.
3ml -185.1 -178.
8 -1 94.
6
64 33.
4ml -182.
8 -176.
5 -1 92.
3
65 33.
5ml -178.
4 -172.1 -1 87.
9
66 33.
6ml -174.
9 -168.
6 -1 84.
4
67 33.
7ml -170.
4 -164.1 -1 79.
9
68 33.
8ml -168.
4 -162.1 -1 77.
9
69 33.
9ml -164.
8 -158.
5 -1 74.
3
70 34.
0ml -161.
6 -155.
3 -171.
1
71 34.1ml -159.
8 -153.
5 -1 69.
3
72 34.
2ml -158.
5 -152.
2 -168
73 34.
3ml -157.1 -150.
8 -1 66.
6
74 34.
4ml -156 -149.
7 -1 65.
5
75 34.
5ml -155.
9 -149.
6 -1 65.
4
76 34.
6ml -155.
3 -149 -1 64.
8
77 34.
7ml -154.
7 -148.
4 -1 64.
2
78 34.
8ml -154.
2 -147.
9 -1 63.
7
79 34.
9ml -153.
9 -147.
6 -1 63.
4
80 35.
0ml -153.
2 -146.
9 -1 62.
7
81 35.1ml -152.
9 -146.
6 -1 62.
4
82 35.
2ml -152.1 -145.
8 -1 61.
6
83 35.
3ml -151.8 -1 45.5 -161.
3
84 35.
4ml -1 51
.1 -1 44.8 -160.
6
85 35.
5ml -150.7 -1 44.4 -160.
2
86 35.
6ml -149.2 -1 42.9 -158.
7
87 35.
7ml -147.9 -59. 6* -156.
7
88 35.
8ml -145.7 -58. 4 -155.
8
89 35.
9ml -142.4 -56. 9 -154.
3
90 36.
0ml -35* -52. 4 -153
91 36.
1ml -33. 8 -48. 5 -152.
6
92 36.
2ml -32. 7 -45 -151.
4
93 36.
3ml -30.1 -42. 5 -150.
9
94 36.
4ml -29. 6 -39. 6 -149.
7
95 36.
5ml - - -148.
6
96 36.
6ml - - -148
97 36.
7ml - - -147.
6
98 36.
8ml - - -146.
8
99 36.
9ml - - -146.
3
1
00 37.
0ml - - -145.
9
1
01 37.
1ml - - -145.
5
1
02 37.
2ml - - -145.1
1
03 37.
3ml - - -144.
6
1
04 37.
4ml - - -144.
2
1
05 37.
5ml - - -143.
8
1
06 37.
6ml - - -143.
2
1
07 37.
7ml - - -142.
6
1
08 37.
8ml - - -142.
5
1
09 37.
9ml - - -142.
2
1
10 38.
0ml - - -141.
9
11
1 38.
1ml - - -141.
6
1
12 38.
2ml - - -141.
2
1
13 38.
3ml - - -141
1
14 38.
4ml - - -140.
9
1
15 38.
5ml - - -140.
7
1
16 38.
6ml - - -140.
4
1
17 38.
7ml - - -140.1
1
18 38.
8ml - - -139.
8
1
19 38.
9ml - - -139.
4
1
20 39.
0ml - - -139.1
1
21 39.
1ml - - -138.
9
1
22 39.
2ml - - -138.
7
1
23 39.
3ml - - -138.
2
1
24 39.
4ml - - -137.
7
1
25 39.
5ml - - -137.
4
1
26 39.
6ml - - -137.1
1
27 39.
7ml - - -136.
9
1
28 39.
8ml - - -136.
7
1
29 39.
9ml - - -136.
2
1
30 40.
0ml - - -135.
8
1
31 40.1ml - - -135.
6
1
32 40.
2ml - - -135.
2
1
33 40.
3ml - - -134.
7
1
34 40.
4ml - - -134.
5
1
35 40.
5ml - - -134.
3
1
36 40.
6ml - - -134.1
1
37 40.
7ml - - -133.
8
1
38 40.
8ml - - -133.
5
1
39 40.
9ml - - -133.
3
1
40 41.
0ml - - -133
1
41 41.
1ml - - -132.
8
1
42 41.
2ml - - -132.
5
1
43 41.
3ml - - -132.
2
1
44 41.
4ml - - -131.
8
1
45 41.
5ml - - -131.
5
1
46 41.
6ml - - -131.
3
1
47 41.
7ml - - -131.
1
1
48 41.
8ml - - -130.
7
1
49 41.
9ml - - -130.
4
1
50 42.
0ml - - -130.
2
1
51 42.1ml - - -129.
8
1
52 42.
2ml - - -129.
6
1
53 42.
3ml - - -129.
3
1
54 42.
4ml - - -129.1
1
55 42.
5ml - - -128.
9
1
56 42.
6ml - - -128.
7
1
57 42.
7ml - - -128.
6
1
58 42.
8ml - - -128.
3
1
59 42.
9ml - - -128.1
1
60 43.
0ml - - -127.
7
1
61 43.1ml - - -127.
5
1
62 43.
2ml - - -127.
2
1
63 43.
3ml - - -126.
5
1
64 43.
4ml - - -126.
3
1
65 43.
5ml - - -125.
8
1
66 43.
6ml - - -125.
2
1
67 43.
7ml - - -124.
8
1
68 43.
8ml - - -124.
5
1
69 43.
9ml - - -124.
2
1
70 44.
0ml - - -123.
9
171 44.
1ml - - -1 23.4
172 44.
2ml - - -123.1
173 44.
3ml - - -1 22.6
174 44.
4ml - - -1 22.2
175 44.
5ml - - -121.8
176 44.
6ml - - -121.2
177 44.
7ml - - -1 20.9
178 44.
8ml - - -1 20.6
179 44.
9ml - - -1 20.3
180 45.
0ml - - -119.6
181 45.
1ml - - -119.1
182 45.
2ml - - -118.7
183 45.
3ml - - -118.5
184 45.
4ml - - -118.3
185 45.
5ml - - -117.8
186 45.
6ml - - -117.5
187 45.
7ml - - -117.1
188 45.
8ml - - -116.9
189 45.
9ml - - -116.5
190 46.
0ml - - -116.2
191 46.
1ml - - -115.8
192 46.
2ml - - -115.4
193 46.
3ml - - -114.7
194 46.
4ml - - -114.3
195 46.
5ml - - -113.9
196 46.
6ml - - -113.3
197 46.
7ml - - -112.9
198 46.
8ml - - -112.5
199 46.
9ml - - -112.1
200 47.
0ml - - -111.8
201 47.
1ml - - -111.4
202 47.
2ml - - -110.6
203 47.
3ml - - -1 09.8
204 47.
4ml - - -1 08.6
205 47.
5ml - - -1 07.5
206 47.
6ml - - -1 06.9
207 47.
7ml - - -1 05.7
208 47.
8ml - - -1 04.9
209 47.
9ml - - -1 03.2
210 48.
0ml - - -23. 8*
211 48.
1ml - - -23. 5
212 48.
2ml - - -22. 9
213 48.
3ml - - -22. 2
214 48.
4ml - - -21.1