SAUPLING AND ANALYSIS OF CONIMERCIAL FATS AND O1LS AOCS& AOCS Official Method Cd 18.90 Reed 2001 p-Anisidine Value DerinTioN "heparin ves defined by coneedon a 100 ms he pea ny mesa 33008 Lm cavetofasoluanconainag 100, ofthc on 100 ml ofa mixure of sdveneand regen according Sermo ded SCOPE Thar daumin han sy ea 3-San a4 iain sees anol acon Inn aes elton of he aldehyde coronas an lo pe se Now, am tht esuag te arb e350 APPARATUS. 1. "Ts tubet—10 ml. mln, wth ether round. gl stoppers or Telloe™ lined stew 2 Yolen fale 35m ns Somes sores ~ 3 Avtomae pipe or automate ures, "Nar—Any pipet andlor br capable ofdlcring rat | mL and 5 ml. satefactory 4. Specoophorontter suet for bueratin 21350 am. 5: Glas chveiee 1,004 0.0 es the two ction ofeach pir mus be enti REAGENTS 1. Ioctane (2.2 4-erimethypemane)—opticaly lear (see Nove, Caution andl 2). 2 Glocial acetic sekd—analyticl reagent quality ise Note. 3). 3: pAitine oa gent quit Ge Noe Cor ad 005 f100 eon in galas espn 2 ote, 5) PROCEDURE ‘xe The ret txmple should be dear and dey (ee Noes, 3). 1 Weigh 0.5-4.0 0.001 y of he va saznpl imo 25 ma. woiumcetc ask Dislve and ine o vohume with henetane 2 Meare the absorbance (Ab) ofthe solution t 350 am in a eivete with the spectophowomcter, wing dhe reference eweete filed with caveat asa bln, 5. Pipe exactly 5 mi of the Fe colution ine one tet ube (Appar 1) and exactly 5 mi of he splveneinzo a second est tube. By means ofan automatic pipet (Apparatus, 3) sl emily 1 mi ofthe pankidine reagent (Reagents 3) to each tube, and shake (ee Nore. 6). 4 Aferexzaly 10 min measure she absorbance (As) ofthe sohent in she sree mbe in a ewe (Apparaus, 5) 2350.0, ‘wing the solution fom she second test eabe a blank in the weference cuvette CALCULATIONS. “The p-anhiine vi (pA. gen by the irl payee BX U2N- AD Where “he sbcorbanc of thet sltion aeration th the panne ment Reagents 3) biochaneof he sion rn mas othe se poton Pago? otARRING AND ANAL OF CONGR SANT OLS Cd 18-90 « p-Anisidine Value PRECISION (see References, 2) Gude Raped OF Refined Palin ‘Tos Sample! Ton'Sample? —TeSample "Tet Semple? No. oflabe 2» 20 2 0 Meanvalie 20 20 23 23 Repeal 40 33 48 46 Reprduelays CY, 9 = 2 20 3 Notes Cestion Aeocrine ie msble and he, Eploce ies a alee 11-6096, ek okey gen nd Ina, A [Pope epetng fie hood doubted when wong wih teh. Ain petals ho Wes fen sin and ste. The Pranic fa rin ad shal be bled wih care rf «Fs ood: p-Assdine an atomic aning « clef tole and ponihycatdnogei hel. o-habidae i oinogen fre and es ong sity axcoames ot ‘plana (Fouts Ania Rapors on Cominogsn NIB 85-008, 085.P-% Chem. Ra Teta df (O91). The TY OL ppm NUMBERED NOTES 1. the precnce of act ak puanisiive races wi aldehydic eompounds in ods of, The imesiy oF colt of the Jillowihrearion produce formed depends not only onthe aount of akchyde compounds peccat but lo on thet race Icha bean found haa doublcbond inte cabon cain conjugated withthe cary double bond ince de wa iapunciou ret The am hued ut peal wl ne bata w Be 2, Terme ene -heemecn be miu fr loorrane at aolvenr, Hlowsie cll containing high anoane of ortiz fry acts il noc dsolve comply in rherane Fr nc ols boocrae shoul be wid a che olen, The dscbance of te sphent used tinocane or fresnel mearured in 1,00 cr euerte brewer 300 and 380 nun, ust il or ney al ‘The commnretd product can be ez Fen abcrpeve maser by poralatngithough aga column (3-5 cm id and 100 er long) Fla with ica el 4am gpm and ia tama foe Hees preity of iso tho tat rp lane Incomplete reaction and, consequent bw vt, Seceglcaet acid el Iygretople ite euancla w check fe mohse convene by Marl chet daertaiunen, ihe conearencede 01 pee, ‘Be acede ac! mut be dacarded 4. essonge, paid esto dain aa evr of oration, The paniiine crysels, which howl! be cian colored, shouldbe sored at OFC ina dark horde, The pv should nse be exposed w Soong light and should be vod below {ay eaor changes observed. A dacoloed agent can be reluced and dovaonzed nthe flowing way. Dore 40 g oF DPrantidine in 100 ml of moter a 75°C, Add 0.2 goFeodium culphie and 20 gof active cabo and sor fr 5 min Then fier though double iter paper. Icarbon pa shrug, repetflraton, Coal the lead colon to about OC alon twstnd a this empetmnm far arleag Shox prea orscghe Flker off he cnveiiued panladine nd wath wih 3 Seal anc afar cepemcetaw/O Ate neg ude sa ch be bt, If sored i the dak and arr expert, the crak oeainel should ner daken precy xy. 5, Reagent solaion avin an abcrbance grater han 0.20 whe measured ina 1 OD om covet at 39D nm against oocare rirhocne aya blank should be ducer. 6, The mimuredould be completely homogenad rch minioum shaking nd then allowed wo rece fr 10 min before pro ‘seding vith the absobance meaturement afore, 4) REFERENCES 1. TUPAC, Suadard Mesh for the Anais of Ott Pas nd Deivatves. 7b ex. Method Number 2.504 Determination of the ‘pranisdine value (p-A.V), Blackall Scientific Publications, Boson, MA ard Oxford. UK (1987). 2, FOSEA International Collaborative Seady #P15, May 1986, Deeumene No. 384, ISO(TC 34/SC 11, February 12, 1987. 3, HOGS 51:17 (1974). 4 Hamilton, Ry.,and S. Hamilon, Lipid Analy, Oxford Univeney Pros, Now York, 1992, pe. 45-47. ge 2072