PO eae uted Neworinglinsiities UndeqiEOly PDA\Collegejof Endineering)|culbaroaBiopolymers: Evaluating their Applications in Food Processing Industry K. Naganagouda and V. H. Mulimani Department of Biochemistry, Gulbarga University, Gulbarga, $85106.Kamataka, India Biopolymers like Sodium alginate; gelatin and Combination of these two (gelatin blended alginate fibers) were employed in enzyme a-galactosidase immobilization studies. Immobilized enzymes have advantages compared to their free counterparts, as they can meet catalytic (expressed as productivity, space-time yield, stability and selectivity) and noncatalyticneeds (¢.g, separation, control, down-streaming process). In the present study, a-Galactosidase from Aspergillus oryaae was physically entrapped in Calcium alginate; gelatin and gelatin blended: alginate hydrogel fibers hardened with using bifunctional reagents such as glutaraldehyde and formaldehyde. Immobilization yield resulted in 45.5, 64.3 and 71.75% respectively. The kinetic parameters like effect of pH, temperature, thermal and pH stability, storage and operational stability of free and immobilized forms were studied, Immobilized a-galactosidase was ‘more stable at higher pH and temperature. Raffinose oligosaccharides (RO) are the major factors responsible for flatulence following inges- tion of soybean-derived products. Removal of RO from seeds or soymilk would then have a positive impact on the acceptance of soy-based foods. In this study, Immobilized ca-galactosidases were used in batch, repeated batch and continuous mode to degrade the raffinose oligosaccharides present in soymilk. ‘The performance of immobilized a-galactosidase was also tested in a fluidized bed reactor at different flow rates and 93% reduction of RO in soymilk was obiained at 25 ml flow rate. The study revealed that alginate-gelatin fiber entrapped a-galactosidase shows the higher immobilization yield, greater stor- age stability and better pereent hydrolysis of RO present in soymilk compared to alginate and gelatin entrapped o-galactosidase. : [prs Polypyrrole Film for the Development of. Ammonia Sensor Kharat HJ, Kakde KP, Savale PA, Datta K, Ghosh Pand Shirsat MD * Optoclectronies and Sensor Research Laboratory, Department of Physics (Electronics), De. Babasaheb Ambedkar Marathwada University, Aurangabad - 431 004, Maharashtra, India e-mail: mds_bamu@yahoo.co.in * Keywords: Polypyrrole; p-toluene sulphonic acid; ammonia sensor Potypyrrle film (doped with p-toluene sulphonic acid) was deposited on glass substrate by chemi- cal oxidation of pyrrole at room temperature Synthesized film was characterized by seaning electron Tmicroscopy and ultraviolet-visible spectroscopy. Sensing behavior ofthe film for ‘ammonia gas was studied by indigenously developed gas sensing chamber. The synthesized fillm shows excellent and reproducible response when it was exposed to 100 - 300 ppm of ammonia gas at room temperature. 0 ———————— AMS - 07 - 27th - 28th January 200.PP9 ee of Ammonia Sensor using Poly (aniline) Film Doped with Acrylic Acid K.P. Kakde, H. J. Kharat, P. A Savale, K.Datta, P.Ghosh, R.D. Mhaske and M. D. Shirsat* Optoelectronics and Sensor Research Laboratory, Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad — 431 004, Maharashtra, India Keywords: conducting polymer, polyaniline, chemical polymerization, acrylic acid, primary dopant, PMMA substrate, gas sensing. In the present investigation we have developed ammonia sensor using polyaniline film doped with acrylic acid. We have synthesized polyaniline by oxidative polymerization of aniline using ammonium peroxydisulfate on poly (methyl methacrylate) substrate in the presence of acrylic acid as a primary dopant without using other acids. The synthesized films were characterized by using UV-Vis Spectros- copy, FTIR spectroscopy, Scanning Electron Microscopy (SEM) and the electrical conductivity. The sensing behavior of the synthesized films for ammonia was studied by using indigenously developed computer controlled gas chamber. The synthesized polyaniline films show excellent sensing behavior for 50, 100 and 250 ppm of ammonia gas. [PPI] Development of Ammonia Sensor using Poly (aniline) Film Doped with Poly (vinyl sulphonic acid) K. P. Kakde, H. J. Kharat, P. A Savale, K.Datta, P.Ghosh, R.D. Mhaske and M. D. Shirsat” Optoelectronics and Sensor Research Laboratory, Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431 004, Maharashtra, India Keywords: conducting polymer, polyaniline, chemical polymerization, polyvinyl sulphonic acid, PMMA substrate, gas sensing. In the present investigation we have developed ammonia sensor using polyaniline film doped with poly (vinyl sulphonic acid). We have synthesized polyaniline by oxidative polymerization of aniline using ammonium peroxydisulfate on poly(methyl methacrylate) substrate in the presence of poly (vinyl sulphonic acid) for the development of ammonia sensor. The synthesized Polyaniline films were char- acterized by using UV-visible, FTIR, SEM and the electrical conductivity. The ammonia sensing behaviour of the synthesized film was studied by indigenously developed computer controlled gas chamber. The synthesized PANI film shows excellent sensing behavior for 50, 100 and 250 ppm of ammonia. MS-07 - 27th - 28th January 2007 a4