Additional Growth Questions:

1. A bacterial culture growing exponentially contained 3.2 x 10 4 CFU/mL at t =

18 minutes. At t = 60 minutes, the CFU/mL were 1.7 x 10 5. Calculate the
CFU/mL of the original culture, at t = 0. (Hint: first find G).

2. A culture of E. coli was inoculated with 2.5 x 10 2 cells at t = 0. After 20

minutes of exponential growth, there were 6.3 x 10 3 cells. Assuming
exponential growth continues throughout the experiment, how long will it take
for the culture to contain 7.5 x 105 cells?

3. The generation time of a Bacillus culture is 24 minutes. 5.0 x 103 cells were
inoculated into fresh media at t = 0. If exponential growth begins after 10
minutes, how many cells will there be at t = 75 minutes?

Answers:
1. 1.6 x 104 CFU/mL
2. 50 minutes
3. 3.3 x 104 cells