MASTER MINIMAL LA +RIF LA +AMP LA +KAN MCA

UNKNOWN PLATE MEDIUM (M3) phenotype

(M4) (M5) (M6)
(M1) (M2)
1 + + + + - + kans prototroph
2 + + + + - + kans prototroph
3 + + - - - + prototroph
4 + + - - + + kanr prototroph
5 + - - - - + auxotroph
6 + - - - - + auxotroph
7 + - - + + + rifs auxotroph
A- E. coli 1366 + + + + - + kans
B- E. coli DH5a + - - - - + wild type
D- S. marcescens + + + - - + rifr
E- P. aeruginosa + + - + + - ampr kanr
F- mutH + - - - + + kanr
G- mutL + - - - - + wild type
H- mutS + + - - - + prototroph
AUXOTROPH + + + - + + amps prototroph
TRANSFORMANT + - - + - + ampr auxotroph
 identity

A- E. coli 1366
A- E. coli 1366
H- mutS
H- kanr mutS
G- mutL
G- mutL
E- P. aeruginosa mutant