Data Gathering Tools

Data obtained was expressed as mean or grand mean. The zones of inhibition were
measured. Ketoconazole were used as control. According to Alalor, C et. al. 2012, the bacterial
and fungal response to the extract was evaluated using the diameter of the zones of inhibition
as follows; Resistant: 10mm and below, intermediate: 11-15mm and susceptible: 16 mm and
above.

Data Gathering Procedure

Plant Extraction

The leaves of Portulaca grandiflora(Rosas de alas diyes) were collected from a

flower store in Pateros, Philippines on September, 2019. The plant sample was identified and
authenticated at the Bureau of Plant Industry, Manila, Philippines. The collected leaves were
cleaned from unwanted materials and dry heat in the oven.

Microbial Media, Chemicals and Standard drugs

Mueller Hinton Agar(MHA) was obtained from the central laboratory Health and
Physical Science Building, University of Makati, Makati City Philippines. Ketoconazole cream
was purchased from the community drugstore, and all other chemicals are obtained from the
local supplier. Candida tropicalis, the microorganisms used for this study, the fungi were
cultured and maintained by using Mueller Hinton Agar.
 Preparation of Methanolic Extract

Preparation of methanolic extracts of Portulaca grandiflora(Rosas de alas diyes)

leaves. The leaves were dried and powdered using a mortar and pestle. Triturated leaves of the
Portulaca grandiflora was extracted with methanol by maceration for 48 hours. The extract
was filtered and concentrated using a rotary evaporator with the temperature of 37°C to
separate the solvent.

Preparation of Ethanolic Extract

Preparation of ethanolic extract of Portulaca grandiflora(Rosas de alas diyes) leaves.

The leaves were dried and powdered using a mortar and pestle. Triturated leaves of the
Portulaca grandiflora was extracted with ethanol by maceration for 48 hours. The extract was
filtered and concentrated using a rotary evaporator with the temperature of 37°C to separate
the solvent.