Which of the following statements is true

A) Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand
B) Bacteriophage lambda exonuclease removes terminal phosphates
C) Alkaline phosphatase removes nucleotides from 5‘ends
D) Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplex
ANSWER: A

Which of the following can be termed as a restriction modification system

A) Restriction endonuclease and methylase
B) DNA ligase and methylase
C) Restriction endonuclease and acetylase
D) DNA ligase and acetylase
ANSWER: A

Which of the following does not act as a restriction enzyme

A) EcorI
B) BamHI
C) HindIII
D) polydeoxyribonucleotide synthase
ANSWER: D

Which of the following enzyme is responsible for making a DNA copy from RNA
A) Reverse transcriptase
B) DNA polymerase
C) RNA polI
D) RNA polII
ANSWER: A

Which of the following is true about restriction endonucleases?

A) Type I and II requires ATP to move along DNA
B) Type I, II and III requires ATP to move along DNA
C) Type II requires no ATP and cleaves DNA within recognition sequence
D) Type II requires ATP and cleaves DNA within recognition sequence
ANSWER: C

Which of the following enzymes’ combined action leads to the generation of sticky
ends in plasmid vector
A) Alkaline phosphatase and terminal transferase
B) Exonuclease III and alkaline phosphatase
C) Bacteriophage lambda exonuclease and terminal transferase
D) Exonuclease III and terminal transferase
ANSWER: C

To make the recombinant plasmid permeable to DNA molecules, which of the chemicals
is added
A) MgCl2
B) CaCl2
C) NaCl
D) HCl
ANSWER: B

Which of the following statements is false

A) The cells which take up recombinant plasmids are drug resistant
B) The cells with no recombinant plasmids are drug resistant
C) Plasmid replication is independent of cell’s division
D) The cells containing recombinant plasmid are called transformed cells
ANSWER: A

The extra chromosomal, self-replicating, closed, double stranded and circular DNA
molecule is generally termed as
A) Chromosome
B) Plasmid
C) Genomic DNA
D) Bacteriophage
ANSWER: B

E.cor1 is a
A) DNA ligase enzyme
B) Restriction endonuclease
C) A vector used for insulin synthesis
D) A plasmid used as a vector
ANSWER: B

In which stage of genetic engineering a probe is used?

A) Cleaving DNA
B) Recombining DNA
C) Cloning
D) Screening
ANSWER: D