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Unit Calculation:
1. Construct a linear glucose standard using the absolute amounts of glucose
(mg/0.5mL) plotted against 540nm.
2. Using this standard, translate the absorbance values of the sample tubes (after
subtraction of enzyme blank) into glucose.
3. Translate the dilutions used into enzyme concentrations;
4. Estimate the concentration of enzyme which would have released exactly 2mg of
glucose by plotting glucose produced against enzyme concentration on
semilogarithmic graph paper.
5. Calulate FPase Unit (FPU):
0.37
FPU = units �ml-1
Enzyme concentration to release 2mg glucose