Tabel Perhitungan Diameter Zona Hambat pada Larutan Uji Sampel

Hasil
Pengamatan
Diameter
Hambat (cm)
Baku
Pembanding Sampel
Rep S1 S3 S2 S3 S4 S3 S5 S3 U S3
1 1,25 1,1 1,125 1,205 2,74 1,11 1,75 1,38 0,52 1,22
2 1,31 1,39 1,05 1,206 2,52 1,305 1,95 1,37 0,5 1,18
3 0,96 1,14 1,15 1,204 2,42 1,33 1,74 1,62 0,51 1,24
4 1,01 1,64 0,66 0,9 2,45 1,33 1,69 1,08 0,71 1,22
5 1,1 1,14 0,73 0,71 2,635 1,215 1,48 1,56 0,87 1,06
6 1,7 1,09 0,68 0,9 2,415 1,214 1,66 1,18 0,74 1,025
7 1,33 1,14 0,8 1,19 2,11 1,405 1,57 1,94 1,29 1,82
8 0,9 1,05 0,6 0,66 2,22 1,01 1,34 1,27 1,64 1,83
9 0,8 1,43 0,6 0,61 2,305 1,08 1,57 1,31 1,22 1,595
Jumlah 10,36 11,12 7,395 8,585 21,815 10,999 14,75 12,71 8 12,19
1,151 1,235555 2,423888 1,22211 1,638888 0,8888888 1,35444
Rata-rata (Y) 11 56 0,821666667 0,953889 889 1 889 1,412222222 9 4
Hasil Koreksi 1,121 2,407722 1,432611 0,7403888
(Y') 5 1,07372222 22 11 9

Y3T = Y3T 1,20594444 Pengolahan

(Y31+Y32+Y34+Y35)/4 Data
Y1'=Y1+(Y3T-Y31) Y1' 1,1215 Dosis Nilai Log S (X) Diamet
Y2'=Y2+(Y3T-Y32) Y2' 1,07372222 S1 6,4 0,806179974
Y4'=Y4+(Y3T-Y34) Y4' 2,40772222 S2 8 0,903089987
Y5'=Y5+(Y3T-Y35) Y5' 1,43261111 S3 10 1
S4 12,5 1,096910013
S5 15,625 1,193820026
 3

a=2.0186 Y3T = 1.20944444 2.5

Diameter Hambat
b = 0.5703 1.20944444 = 2.0186x - 0.5703 2
0.639144444 = 2.0186x 1.5 Series1
x = 0.639144444/2.0186 Linear (Series1)
1
x = 0.316627583
0.5 Linear (Series1)
x = log S,
S= log^-1 X 0
y = 2.0186x - 0.5703
S= 2.073134996 0 0.5 1 1.5
R² = 0.312
Log S

Perhitungan:
(4.456876398 μg/mL : 10μg/mL ) x 100% = 44.56%
 Tabel Perhitungan diameter zona hambat standar pada antibiotic yang ada di laboratorium

Diameter Hambat (cm)

Baku Pembanding Sampel
Rep S1 S3 S2 S3 S4 S3 S5 S3 U
1 1,44 1,715 1,5 1,5 1,8083 1,681 1,95 1,57 1,165
2 1,55 1,71 1,5 1,55 1,8067 1,75 1,94 1,66 1,165
3 1,495 1,78 1,42 1,26 1,803 1,75 1,9 1,69 1,163
4
5
6
7
8
9
Jumlah 4,485 5,205 4,42 4,31 5,418 5,181 5,79 4,92 3,493
Rata-
rata (Y) 1,495 1,735 1,4733333 1,4366667 1,806 1,727 1,93 1,64 1,164333333
Hasil
Koreksi
(Y') 1,394666667 1,6713333 1,7136667 1,92466667 1,583999997

Y3T = Pengolahan
(Y31+Y32+Y34+Y35)/4 Y3T 1,63466667 Data
Log S Diameter Hambat
Y1'=Y1+(Y3T-Y31) Y1' 1,39466667 Dosis Nilai (X) (Y)
Y2'=Y2+(Y3T-Y32) Y2' 1,67133333 S1 6,4 0,8062 1,63466667
Y4'=Y4+(Y3T-Y34) Y4' 1,71366667 S2 8 0,9031 1,39466667
Y5'=Y5+(Y3T-Y35) Y5' 1,92466667 S3 10 1 1,67133333
S4 12,5 1,0969 1,71366667
S5 15,625 1,1938 1,92466667
 2.5

2
Diameter Hambat

1.5

0.5

0
0 0.2 0.4 0.6 0.8 1 1.2 1.4
y = 0.9278x + 0.74
Log S R² = 0.562

Series1 Linear (Series1)

Y= 0,9278x+0,74
1,58397-0,74= 0,9278x
X= 0,9096464
Log s = 0,9096464
S= 8,12169
8,12169µ𝑔
S= 10µ𝑙
x 100% = 81,21%