Name Period +h 20 wel QUESTIONS oH,?, 1 2 7% ree 7 AHO+ OL What was the enzyme in this lab? CotMlase What was the substrate in this lab? He 05 What was the product(s) in this lab? HO 4 ad O, 6. 2 j What variable was measured in this lab? me. If you wanted to determine rate of enzyme activity from the data collected in this lab, how would you do that? a (x -f, ee: prac 20, m F, What are some good sources of the human enzyme catalase? {wer + gees Enzyme concentration a, Summarize the effect of enzyme concentration on enzyme activity. Explain the mechanisms that cause this effect, + rate of reaction Ct sloye,) aot Coenzyme] lower Conume) means les active ez Rewer- ov lors. 3. UWA hy What would cause tie rate of enzyme activity to level off on the enzyme concentration graph? stact +o run cut of subsbadle c. Draw an “idealized” graph to ilustratedtie telationshi between enzyme concentration anfi Yald!sMenzyme FH Rncime} Bor tt 2007-2008Name Period 8. Temperature a, Summarize the effect of temperature on enzyme activity. Explain the mechanisms that rd od i 3 aS Room Teh temp — wore well b. Explain the specific effect of boiling the enzyme. attve sites Roroduced =not worlnR ©. Draw an*ideatized” gran ilustrate the relationship betwoek temperature end rate of enzyme activity. ° 4. What isthe optimal temperature for human enzymes? BEPC 9. pH 2. Summarize the effect of pH on enzyme activity. Explain the mechanisms that cause this, effect. Yacht pth iD highest pit T'S oll f lower J aes T ie ca eye b, Draw an “idealized” graff to ilustrate the relationship between pH and rate of enzyme activity ©. Do all human enzymes function within the same ‘optimal pH range, like they do with temperature? Explain. No = Stach enyes Wie acnt “Ialestones We Lait. blood — neutral Sorit 2007-2008Name Period 10, Substrate concentration a, Summarize the effect of substrate concentration on enzyme activity. Explain the mechanisms that cause this effect. Higher [eubstrale | 9 isha rake, have more oubstrate b. What would cause the rate of enzyme activity to level off on the substrate concentration graph? eee Over tne, ehetade Ts used sup 2h a — activity. ° 11, Jon concentration a, Summarize the effect of ion concentration on enzyme activity. Explain the mechanisms that cause this effect. oO” Workid best at QZo- lovin ot 02 onl 10% Yoetanse lech of ton or te much tm cared ackve. the] encase narge. to be Wsa Reporte b. Draw an “idealized” graph to illustrate the relationship between jon concentration and rate of enzyme activity. Wot it 2007-2008Name Period 12, After an enzyme is mixed with its substrate, the amount of product formed is determined at 40-second intervals for 1 minute. Data from this experiment are shown below. v 7) Time (Sec) To 7, 10] 2 | 3 | 4 | 5 | 60 Productfomed(mg) | 0.00 | 0.25 | 0.50 | 0.76 | 0.60 | 085 r 0.85 a. Whatis thei) jato of this enzymatic reaction? __Q,S-O_. 02S ing pease na ey Show your calculation: Sue) b. What is the rate,Sier50 seconds? Sx ‘Show your calculation: OBS ~O.80 ry preduth BO- 40 sec i c. Why is the rate after 50 seconds different from the initial rate? — Orde Ke, substrate ts used up. Thurber thane leat sualesbrabe. do convert to produch atofti 2007-2008