Professional Documents
Culture Documents
CRIME SCENE
FIRST RESPONSE
GOAL
INTRODUCTION
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1. Gloves
2. Face Mask
3. Preservation line
4. Chalk / yellow chain / nylon cord / straw
5. Camera
6. Pen
7. Note Pad
8. Hair net
9. Foot Cover
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Purpose of FR:
Assess the crime scene
Serve the victim’s need/s; Interview witnesses;
Apprehend the suspect;
Secure, Protect and Preserve Evidence
Proper turn-over to the IOC
ROLE OF FR
Crime Scene Safety
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Dying Declaration
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POLICE LINE_
50% +50%
Approx.
distance.
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POLICE LINE_
50% +50%
Approx.
distance.
POLICE LINE_
OSCP,Political Figures,
Gov’t/local officials,
Public/Crowd
Media
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Identify witness(es)
Where is/are the victim(s)?
Where is/are the suspect(s)?
-Arrest or Pursue Suspect(s)
When suspect(s) is/are fleeing, make appropriate
notification for danger and dragnet operations.
RECENT 2012
MOST RECENT
2013
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Turn-over to IOC
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PART 2
FR PROTOCOL FOR
EXPLOSIVES RELATED
INCIDENTS
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DISCOVERY OF IED
• On the discovery of IED/s in an area, after the
conduct of EOD Unit shall call the assistance of local
police unit and SOCO Team who has jurisdiction on
the area.
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Specific Functions,
Responsibilities & Procedures
INVESTIGATOR-ON-CASE
(1) Upon arrival at the crime scene, the IOC shall
request for a briefing from the FR and make a quick
assessment of the crime;
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Specific Functions,
Responsibilities & Procedures
INVESTIGATOR-ON-CASE
(3) Based on the assessment, if the IOC determines that
a SOCO team is required, he shall report the matter to
his COP and request for a SOCO, otherwise, the IOC
shall proceed with the CSI without the SOCO team and
shall utilize CSI Form“4”;
Specific Functions,
Responsibilities & Procedures
INVESTIGATOR-ON-CASE
Specific Functions,
Responsibilities & Procedures
INVESTIGATOR-ON-CASE
(7) Upon the arrival of the SOCO Team, the IOC shall
accomplish CSI Form “2” and submit the same to the
SOCO TL. SOCO team shall not enter the crime scene
unless the IOC makes the official written request
wherein he assures the SOCO team of his presence and
support
(8) IOC shall brief the SOCO Team upon their arrival at
the crime scene and shall jointly conduct the
preliminary crime scene survey.
Module 1 Lesson 1.3 51
Rev: Nov 2016
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Specific Functions,
Responsibilities & Procedures
SOCO TEAM
Specific Functions,
Responsibilities & Procedures
SOCO TEAM
Specific Functions,
Responsibilities & Procedures
SOCO TEAM
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Specific Functions,
Responsibilities & Procedures
SOCO TEAM
(5) SOCO Team shall accomplish the CSI Form “4” &
furnish the IOC of copies of the same before leaving the
CS.
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TANONG?????
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FORENSIC CHEMISTRY
EXAMINATION
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
7/21/2017
FORENSIC CHEMISTRY
EXAMINATION
Forensic Chemistry
Forensic Toxicology
Is the use of toxicology principle to aid medico legal
investigation of death and poisoning.
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Forensic Chemistry
Forensic Chemist – licensed chemist, is a person who is
expert is forensic chemistry tasked to analyze the chemical
specimen using analytical methods available
Founding Father
of Forensic Chemistry and Forensic Toxicology
Prof Matheiu Orfila, chemist, is the father of modern
forensic Toxicology after testifying in court as expert
witness against Madam Marie Lafarge, after poisoning
her husband, Charles Lafarge with Arsenic.
He uses the chemical technique “Marsch test” to identify
arsenic from the body of Charles LaFarge.
Image: Photo of Sebastian Sanchez Palomares (wearing a hat), Medico Titulares of Pampanga –
1897 Source: Todocoleccion.net
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Philippine Drug
Enforcement Agency
Bureau of Customs
Police
Philippine Coast Guard
National Bureau of
Investigation
Other investigative agency
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6. Seal end
3. Fold bottom up
with tape
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1 One (1) Heat-sealed -Transparent plastic sachet With markings Containing White crystalline
Tape-sealed -Transparent plastic bag “ABC 8/16/13” substance
Knot-tied -White plastic bag
Unsealed
Staple-sealed
Self-sealing
3 Three (3) Blister Labeled “medicol” With markings Containing three (3) red tablets/
packed/ (C1,C2, and C3) Containing three (3) blue and white
Foil packed respectively colored capsules.
4 One (1) Improvised plastic tooter With markings Containing Traces of white crystalline
“C” substance
5 Two (2) folded Strips of aluminum foil With markings Containing Traces of white crystalline
(D1 and D2) substance
respectively
As an expert witness, the analyst presents his or her findings in a court of law.
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Disposal
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RA 9165
I. Sec 15 of RA 9165: Use of Dangerous Drugs
A person apprehended or Penalty
arrested who is found to be 1. First offense: Min of 6 months
POSITIVE for dangerous drug rehabilitation
after confirmatory exam 2. Second offense: imprisonment
from 6 years and 1 day to 12 years
and fine of P50T to P200T.
Classification by Origin
NATURAL PRODUCT – extracted from plants, animals or
living material.
Examples: opium, cocaine, THC, Ephedrine, Mescaline, LSD
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Classification by Origin
SEMI-SYNTHETIC – made from natural source but undergone
chemical process.
Examples : Heroin, Hormones and Steroids
Extraction Acetylation
Opium
Morphine Heroin
Classification by Origin
2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012
Meth Meth Meth Meth Meth Met Meth Meth Meth Meth Meth
Chloro- dimeth Dimeth dimeth dimeth Chloro- Ethylam- dimeth
meth Chloro - Chloro - Chloro - meth phetamine
meth meth meth
Cocaine Cocaine Cocaine Cocaine Cocaine Cocaine Cocaine Cocaine Cocaine Cocaine
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Drug sampling
Sampling Advantages
Disadvantage
1. All (n = N) A: 100% certainty of composition of population.
D. Excessive sample size
2. Percent, n = 0.05N, 0.10N etc. A: simple method.
D. Excessive sample size
3. Widely used, n = √N, n = 0.5 √N, n = √N/2 A: widely accepted approach.
D. Number of sample is too small from small population.
Excessive sample size for larger population.
4. n=20+10%(N −20) (where N >20 ) A: Heterogeneous populations likely to be discovered
before analysis is complete.
D. Excessive sample sizes for larger populations.
5. For N < x, used n = N. A: United Nations Drug Control Programme
For x ≤ N ≤ y, (11 to 99) used n = z (10). (UNDCP) recommended method ( x =10, y =100, z =10).
For N > y, used n = √n D: Excessive sample sizes for larger populations.
Sampling Method
• scooping from the top
• Sample thief
• Coning and quartering
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AMPHETAMINE TYPE
Stimulants(ATS)
And newly emerging drugs
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History of Methylamphetamine
Hydrochloride (shabu) manufacture
History of Methylamphetamine
Hydrochloride (shabu)
manufacture
It is since 1941 during the World
War 2 that Methamphetamine
had been manufactured and sold
for medical supplies in Japan.
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• 1987 2009
– Marijuana (1310 cases) – Marijuana (1310 cases)
– Methamphetamine (50 cases) – Methamphetamine (24,768.13 grams)
– Cocaine (5 cases) – Cocaine
– Codeine (1 case) – Heroin
– Valium (5 cases) – BZP
– Mandrax (3 cases, 980 tab, 12 tab, 1 tab) – ecstacy
– Heroin (3 cases)
– Rugby (6 cases)
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Paint
layer
Marijuana cystolith
Cotton fiber
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• Confirmatory
Marijuana Color Extract Rf values Extract Rf values
Component using using
s methanol Ethanol
Trial 1
Cannabidiol orange 2.7 0.32 3 0.35
Cannabinol purple 2.2 0.26 2.5 0.29
Delta-9-THC Red 1.9 0.22 2.1 0.25
Trial 2
Cannabidiol orange 2.8 0.33 2.8 0.33
Cannabinol purple 2.4 0.28 2.4 0.28
Delta-9-THC Red 2.0 0.24 2.0 0.24
Infrared Analysis
Within 24 hours,
initial and final reports shall be prepared by the
chemist
Take note of the following:
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Paraphernalia
(label sharp for sharp paraph)
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Ecstacy Paraphernalia
- Vicks Vapor rub/ nasal inhalers • Most users experience
- Baby pacifiers/ candy suckers
- Dust masks/ tampon tubes with dehydration.
Vicks • Enjoy seeing bright lights
- Glow sticks
- Blow pops
IN NO WAY ARE WE SAYING THAT ALL PEOPLE WHO EXHIBIT SOME OF THESE BEHAVIORS OR
POSSESS THE ABOVE ITEMS, ARE ECSTASY USERS.
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Definitions
• Explosion – broadly defined as the sudden
rapid escape of gases from a confine space,
accompanied by high temp, violent shock and
loud noise.
• Explosive – a substance, chemical compound
of mixture that under the influence of heat,
pressure, friction or shock undergoes a
sudden chemical change or decomposition.
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Explosives
• High Explosives • Military Explosives
– Primary – Grenade
– secondary – Submunitions
• Low Explosives • Industrial Explosives
– Primer
– Igniter
– propellant
Explosives RRDK
Rapid Response Deployment Kit
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Useful in detecting
explosive mixtures using a
sensitive separation
technique and a detector.
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Smiths IdentifyIR
IdentifyIR® is a rugged,
miniaturized FT-IR
(Fourier Transform
Infrared) analysis system
that is fast, easy-to-use,
and accurate.
- can handle powders,
liquids, solids, gels &
pastes in both organic &
aqueous solvents.
FORENSIC
CHEMISTRY 3. Conduct examination of firearms
1. Drugs
2. Explosives and paraffin casts for the presence of
3. Gunshot
residue gunpowder nitrates.
4. Distance
determination - casting shall be done
5. Toxicology
6. Fake Products within 72 hrs from the
7. Blood Alcohol
8. Drug Test
time of alleged firing,
9. Clandestine Lab
Invest
otherwise the request
10. Trace Evidence shall be denied.
11. Lecture
12. Research
13. Testify
14. Advice &
Consultancy
15. Other function
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SEM-EDX
• Scanning Electron
Microscope with
Energy Dispersive
Xray Analysis
FORENSIC
CHEMISTRY 4. Conduct gunpowder examination of
1. Drugs
2. Explosives clothing for possible gunshot range
3. Gunshot residue
4. Distance determination.
determination
5. Toxicology
6. Fake Products
7. Blood Alcohol
8. Drug Test
9. Clandestine
Lab Invest
10. Trace Evidence
11. Lecture
12. Research
13. Testify
14. Advice &
Consultancy
15. Other function
Distance Determination
Classification
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FORENSIC
CHEMISTRY 5. Conduct chemico-toxicological examination of
1. Drugs human internal organs, gastric contents, blood, water
2. Explosives
3. Gunshot residue
and food samples for the presence of chemical
4. Distance poisons.
determination
5. Toxicology - Require standards
6. Fake Products
7. Blood Alcohol
8. Drug Test
9. Clandestine
Lab Invest
10. Trace Evidence
11. Lecture
12. Research
13. Testify
14. Advice &
Consultancy
15. Other function
Poison Investigation
• Most important is the sight and smell of
scene.
• Does the position appear “poisoned”?
Two studies suggest that drug absorption is lowest in patients lying on their left side,
so you might want to consider placing an unconscious overdose patient in the left-
sided recovery position prior to definitively managing them in hospital.
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Poison Investigation
• Most important is the sight and smell of
scene.
2. The skin and mouth, lips, rectum, vagina and
genitals?
Poison Investigation
• Most important is the sight and smell of
scene.
3. The pupils of the eyes...
Poison Investigation
• Most important is the sight and smell of
scene.
4. Odors present
5. Possible skin marks via hypodermic needle
injections.
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Poison Investigation
• Most important is the sight and smell of
scene.
6. The hands for the presence of objects.
Food Poisoning
• Symptoms of poisoning appeared soon after a
drink or meal taken.
– the investigator should thoroughly see that all
liquids, foods, and medicines on the premises are
preserved.
– These can be found at the medicine cabinet,
pantry, refrigerator, and even the reuse container.
Food Poisoning
• If many hours have elapsed after the meal was
ingested, the possibility of food as the source of
poison may be eliminated.
– When symptoms of poisoning occur, the investigator can
reasonably assume that the victim had taken the poison
from on-half to one (1/2 to 1) hour before the first
symptoms appeared.
– In corrosive poisons, symptoms appeared immediately.
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Preservation of evidence
• The evidence (as biological in origin) can be
preserved in a plastic or glass container and
stored in freezer 100C or below. Sample can be
discarded according to the laboratory policies
and SOP’s.
FORENSIC
CHEMISTRY 6. Perform laboratory examination of fake
1. Drugs
2. Explosives
products in comparison with standard
3. Gunshot residue
4. Distance
specification for cases of unfair trade
determination
5. Toxicology competition. (ex Fake fundador, adulterated
6. Fake cement, adulterated beer, fake chlorox, fake
Products
7.
8.
Blood Alcohol
Drug Test
cigarettes).
9. Clandestine Lab
Invest
10. Trace Evidence
11. Lecture
12. Research
13. Testify
14. Advice &
Consultancy
15. Other function
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FORENSIC
CHEMISTRY 7. Conduct blood alcohol determination.
1. Drugs
2. Explosives Types of alcoholic beverages:
3. Gunshot residue 1.Fermented – low alcoholic content, example beer =
4. Distance 4-6% ethanol
determination
5. Toxicology 2. Wine – 8-14%
6. Fake Products 3. Distilled Spirits – high alcoholic content
7. Blood a. 100 proof = 50% ethanol
Alcohol b. 80 proof = 40% ethanol
8. Drug Test
9. Clandestine Lab 4. Combination (fortified, port & sherry wines)
Invest
10. Trace Evidence
11. Lecture A person is said to suffer from alcohol intoxication when the quantity
12. Research
13. Testify of alcohol the person consumes exceeds the individual's tolerance
14. Advice & for alcohol and produces behavioral or physical abnormalities.
Consultancy
15. Other function
In other words, the person's mental and physical abilities are
impaired. The person can't function and certainly should not be
operating a motor vehicle. It acts on the forebrain then to the central
and rear portion are affected.
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2. Breath analysis
FORENSIC
CHEMISTRY 8. Examine urine and other body
1. Drugs
2. Explosives fluids for drug metabolites.
3. Gunshot
residue • PNP Mass promotions
4. Distance
determination • PNP Recruitment
5. Toxicology • PNP Re-enlisment
6. Fake Products
7. Blood Alcohol • Persons apprehended under RA 9165
8. Drug Test • PNP firearms License/Permit to Carry = 2546
9. Clandestine
Lab Invest • Other government employee under drug
10. Trace investigation
Evidence
11. Lecture
12. Research
13. Testify
14. Advice &
Consultancy
15. Other
function
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The substance being tested, testing method, and levels tested for are major factors.
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Drug Test
Two (2) stages of examination
1. Screening using test kits
detection limit: Meth = 1000 ng/ml
MJ = 50 ng/ml
C C C C C
M M M M M
T T T T T
Drug Test
2. Confirmatory
a) Thin Layer Chromatography
b) Instrumentation-Gas Chromatography
Mass Spectroscopy (GCMS)
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Legal Aspect
• Comprehensive dangerous drug Act of 2002
– First offense: min of 6 months rehab
– Second offense: 6 to 12 years imprisonment and
fine of 100 to 500T.
FORENSIC
CHEMISTRY
9. Conduct clandestine lab
1. Drugs investigation
2. Explosives
3. Gunshot residue
4. Distance
determination
5. Toxicology
6. Fake Products
7. Blood Alcohol
8. Drug Test
9. Clandestine
Lab Invest
10. Trace Evidence
11. Lecture
12. Research
13. Testify
14. Advice &
Consultancy
15. Other function
Clan Lab
• Small (1-10 kg)
• Medium (11-50kg)
• large scale (51kg above)
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Physical Identification
• Ultraviolet exam
• Macro etching
• Hair fibers and Paint Analysis
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Communication and
Team work…
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ULTRAVIOLET POWDER
EXAMINATION
PNP CRIME LABORATORY
Physical Identification Division
ULTRAVIOLET POWDER
EXAMINATION
PNP CRIME LABORATORY
Physical Identification Division
Common Applications
• Illegal recruitment
• Extortion
• Drug buy-bust operations
OUTLINE of PRESENTATION
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PART
ONE
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MISSION
FUNCTIONS
QUALIFICATIONS OF PI EXAMINER
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Scope of PID
Ultraviolet examination
Bullet trajectory examination
Macro etching examination
Casting and molding examination
Tool marks examination
Paint analysis
Arson examination
Hair and fiber analysis
PGS OVERVIEW
VISION
Imploring the aid of the Almighty, by 2030, We shall be a highly capable, effective and credible
police service working in partnership with a responsive community towards the attainment of a
safer place to live, work, and do business.
Highly Capable, Effective and Credible Police Service by 2030
MANDATE
Republic Act 6975 as amended
by RA 8551 and further
amended by A safer place to live, work
RA 9708 and do business
MISSION
Enforce the law, prevent and
control crimes, maintain peace
Improve crime Improve community safety awareness
and order, and ensure public
prevention and through community-oriented and
safety and internal security
control human rights-based policing
with the active support of the
community.
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PART
TWO
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Applicable to entrapment
and buy-bust operations.
Ultraviolet Light
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UV light . . .
The name means "beyond violet" (from Latin
word ultra, "beyond"), violet being the color of
the shortest wavelengths of visible light. UV
light has a shorter wavelength than that of
violet light.
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UV Light
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Black Light
This range is just slightly below the 400nm that human eyes
can detect. When this light is reflected off certain materials, it
looses energy and its wavelength expands to over 400nm.
Black Light . . .
Standard fluorescent black lights are constructed in an almost
identical fashion to their white-light counterparts.
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Black Light . . .
Black Light . . .
Bodily fluids (such as blood, semen, urine, and saliva) are
phosphor-rich. Criminal investigators have been using
black lights to detect these fluids on crime victims and at
crime scenes.
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Electromagnetic Spectrum
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Sun
Black light
Ultraviolet fluorescent lamp
Ultraviolet LEDS
Ultraviolet lasers
Gas discharge lamps
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Visible light radiates at wavelength (400 and 700 nm) and refers to the
only portion of the electromagnetic spectrum that can be seen by the
human eye. Like other parts of the spectrum, ultraviolet radiation is not
visible to the human eye. It falls beyond the visible violet wavelengths
and radiates at shorter wavelengths, between approximately 100 and 400
nm, or between visible light and x-rays. This portion of the
electromagnetic spectrum can be broken down in the following regions:
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SAFETY
While “black lights” do produce light in the UV range, their
spectrum is confined to the long wave UVA region.
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SAFETY . . .
UVA light can cause DNA damage, but not directly like
UVB and UVC .Due to its longer wavelength, it is absorbed
less and reaches deeper skin layers ( the leather skin)
where it produces reactive chemical intermediates, such
as hydroxyl and oxygen radicals, which in turn can
damage DNA and result in a high risk of melanoma.
Fluorescence
Fluorescence is an optical phenomenon wherein a
material emits light in response to some external stimulus.
Fluorescent light that is emitted is of a specific color or
group of colors that is released when the material is
bombarded with light in some part of the color spectrum.
Fluorescence . . .
These molecules absorb light energy from external
sources, and this energy causes excitation of the electron
orbitals in a process called pi-bonding. When the excited
pi-bonds relax back to a lower energy state, photons of a
specific wavelength are emitted in the process, giving rise
to the fluorescent light.
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Bullet Flu pastes can play a vital part regarding whether or not
wounds received from firearms were caused by officers or other
participating in a riot or other circumstances. Caution should be used
when applying flu pastes to bullets and shells, for coarse granules in
the paste may cause damage to the firearm or to the operator when
the gun is used. Very thin pastes and fine powders generously
dusted within a barrel will usually work well. Prior experimentation
should be made so that each officer will know just how they will
perform.
Censorship Penal institutions scan mail for secret invisible
message and reveal it under UV light.
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Coins At the U.S. Mint, telephone boxes, parking meters and other
vending machines are frequently marked with invisible powders for
entrapment.
Documents Analysis of documents under UV light will bring out
faded signatures, erasures, changes in original copy and invisible
markings or messages, since the latter are generally written with such
fluorescent substances as quinine, lemon juice, egg whites, aspirin,
urine, acetanilide (cold tablets) or specially prepared inks. Document
tampering can be detected by premarking of files with vertical or
diagonal lines of invisible ink.
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Hair Bleached hair will show a characteristic whitish flu. Rodent hairs
have a bluish / white flu. Dyed hair is usually apparent as most hair
dyes are flu. Some hair oils, as well as wigs, also flu, but experience
and practice will enable one to isolate one from the other.
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Lipstick While not apparent under white light, lipstick traces on the
skin will fluoresce red and orange. (Not all lipsticks fluoresce.)
Peeping Toms A thread, two feet high and easily broken, can be
treated with invisible flu powder and stretched around the appropriate
window. Any suspect in the area can readily be checked with a UV light
for the line of fluorescence two feet above ground.
Psychics "Ectoplasm" or spirit faces and other phony props used by
these practitioners are immediately exposed under UV light.
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LABORATORY
DEMO
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powder brush
Ultraviolet lamp
Investigative Procedure
The investigator shall prepare a letter request to the
PNP Crime Laboratory for Ultra Violet Powder
“Dusting” of marked money to be used in the
entrapment operation.
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Investigative Procedure . .
Investigative Procedure . . .
LABORATORY EXAMINATION
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LABORATORY EXAMINATION . . .
LABORATORY EXAMINATION . . .
23
QUESTIONED
DOCUMENTS
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
7/21/2017
QUESTIONED
DOCUMENTS
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
OUTLINE
I. INTRODUCTION
a. Mission
b. Vision
II. FUNCTION
III. REQUIREMENTS
Mission
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Vision
FUNCTIONS
FUNCTIONS
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REQUIREMENTS
Signature Identification:
Four (4) Copies of letter request addressed to D, CL
(Attn: C,QDED)/ Court Order if the case is undergoing
trial or pending in court.
Original Copy of the questioned Document
Original copy of at least eight (8) or more different
documents bearing the standard signatures of subject
individual
(Note:) Standards should be executed 5 years before &
5 years after the date of execution of questioned
signature.
Payment of Php 2, 000.00 for the photographic
materials. (To be paid at Land Bank Camp Crame,
Branch)
REQUIREMENTS
Handwriting Identification:
Four (4) Copies of letter request addressed to D, CL
(Attn: C,QDED)/ Court Order if the case is undergoing
trial or pending in court.
Original Copy of the questioned Document
Original copy of at least four (4) pages or more
extended handwriting of subject individual w/ the same
style of writing.
(Note:) Standards should be executed 5 years before &
5 years after the date of execution of questioned
signature.
Payment of Php 3, 500.00 for the photographic
materials. (To be paid at Land Bank Camp Crame,
Branch)
REQUIREMENTS
Alteration:
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REQUIREMENTS
4
FORENSIC
PHOTOGRAPHY
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
Twenty two (22) Facial Composite System
Donated by the AFP and the PNP Foundation Inc.
FACIAL COMPOSITE
Previously called Computerized Composite Criminal Illustration
or Cartography or Cartographic Sketch
FACIAL COMPOSITE
Missing Person
33
May 2011
FACIAL COMPOSITE
Facial Composite is a graphical representation of
an eyewitness’ memory of face, as recorded by a
composite artist through hand drawing.
34
IMPORTANCE of FCS
The suspect or the missing person can easily be
identified with the aid of facial composite.
35
How much does it cost?
It is absolutely FREE!
How to avail?
36
PROCESS
37
FACIAL IDENTIFICATION FORM
38
FACIAL IDENTIFICATION FORM
39
FACIAL IDENTIFICATION FORM
40
FACIAL IDENTIFICATION FORM
Offense/ Incident: Multiple Frustrated Murder
NAME: UNKNOWN
ALIAS/AKA: Ka Johnny
SEX: Male
AGE: 35-40 years old
HEIGHT: 5’5”- 5’10”
WEIGHT: 60-65 Kg
SKIN COLOR: Fair
BUILT: Small
EMPLOYMENT: Unknown
ADDRESS: Unknown
RELIGION: Unknown
OTHER INFORMATION: Armed with Pistol (cal.45) Wearing SWAT uniform
DESCRIBED BY: Pedro M Perez
REQUESTING PARTY: CIU CIDG
Artist/Interviewer: PO3 __________
ACCOMPLISHMENT
ACCOMPLISHMENT
PICTURE OF THE
FACIAL COMPOSITE SUSPECT
41
ACCOMPLISHMENT
PICTURE OF THE
FACIAL COMPOSITE SUSPECT
CFC Case No : CCCI-031-2012 (July 23, 2012)
Offense : RAPE
Requesting Party : PS#2, Gen Santos City Police Office
Investigator/s on Case: SP02 Rex Diongon
Status of Case : Case is under preliminary investigation.
Suspect was apprehended in one of the drug related crime operations
of the GSCPO-CAIDSOTF.
Name of Suspect/s : Vessie Vegafria Tabiado, 33 y/o, married, laborer and a resident
of Lot 5, Sugod, Apopong, Gen. Santos City
Status of Suspect/s : Arrested
ACCOMPLISHMENT
PICTURE OF THE
FACIAL COMPOSITE SUSPECT
CFC Case No. :CCCI-020-2012
Offense :
Alleged Robbery with Homicide
Requesting Party :
Tupi Police Station, Tupi South Cotabato
Investigators on Case :
PO2 Esteban M Lambino Jr and P01 Ronnie L Janiola
Status of Case :
The case is under trial at the Provincial Prosecutors Office,
Koronadal City docketed under NPS No. XII-089-INV-12E-00206
Name of Suspect/s : Ryan Paquirda y Carriedo
Status of Suspect/s : Arrested
42
CRIME SCENE
SKETCHING
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
CRIME SCENE
SKETCHING
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
METHODS OF SKETCHING
1
In certain types of crime, particularly
those involving physical violence, the crime
scene and the location of all relevant objects
within it are of vital importance in establishing
points of proof.
Coordinate Method
2
COORDINATE METHOD
16’00”
6 6
5
9’9”
9’2”
13’5”
9’6”
1 3 3 6
8’4”
1
2’8”
2
2’4” 3’0” 4’5” 8’3”
16’00”
COORDINATE METHOD
Cross-Projection Method
3
EXPLODED VIEW
CROSS PROJECTION
EXPLODED VIEW
Triangulation Method
4
TRIANGULATION METHOD
16’00
6 6
5
4
13’5”
13’5”
1 3 3 6
1
8’9”
2
2’4” 3’0” 4’5” 7’3”
LEGEND:
2nd Floor .
5
POLYGRAPH
BRIEFING
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
7/21/2017
POLYGRAPH
BRIEFING
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
WHAT IS A POLYGRAPH
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MISSION
CAPABILITIES
Polygraph Examiner
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PERSONNEL
PCO PNCO NUP
20 35 06
TOTAL 61
Criminal Investigation
Pre-employment
Periodic Screening
Insurance Investigation
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PROHIBITED PRACTICES
• The investigator should not subject the examinee to prolonged
interrogation immediately before a polygraph examination.
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Accuracy 90%
Inconclusive 10%
Psychology Department
University of Utah
Funded by the U.S Treasury Department
5
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The best steps for a person to prepare for a polygraph test are:
Step 2: Get a good night sleep. Don’t stay up late watching television or
reading. Be on time in the morning to give you plenty of time to prepared
without rushing.
Step 3: Eat breakfast if your schedule is in the morning. Follow your
normal habit.
Step 4: Plan for a polygraph examination that will take a about 2-3 hours.
This includes pre-test interview which is the longest phase of polygraph
examination to ensure that you are comfortable during polygraph
examination.
Step 5: Know your legal rights.
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TELL THE
“TELL THE
TRUTH
TRUTH”
8
DACTYLOSCOPY
DACTYLOSCOPY
FPID MISSION
FPID FUNCTION
1
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FPID FUNCTION
FPID CAPABILITIES:
FPID CAPABILITIES:
2
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FPID CAPABILITIES:
QUALIFICATION OF A FINGERPRINT
EXAMINER
3
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PRINCIPLES OF
1. PERMANENCY -That fingerprints do not change in
FINGERPRINTS
its ridge characteristics throughout the lifetime of an
individual and they persist after DEATH until the skin is
decomposed.
2. INDIVIDUALITY -That the complexity of the ridge
details of a single fingerprint, or even in part, is not
duplicated in any other finger.
3. INFALLIBILITY - That fingerprints are incapable of
error, “Cannot commit mistakes”
11
IMPORTANCE OF FINGERPRINTS
Prevent impersonation.
Speedy identification of wrongdoer.
Serve to give evidence.
Help identify victim/s of disasters,
calamities, floods etc.
Identifies bodies, mutilated beyond
recognition.
Aids the judiciary in penal treatment.
Prevent criminal substitution of newly
born.
Identification of falsified document.
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DEFINITION OF FINGERPRINT
As an IMPRESSION:
It is the reproduction on some smooth surface
of pattern or design formed by the ridges on the
inside of the end joint of the fingers through the
medium of ink or any coloring substance capable
of producing visibility.
As a SCIENCE:
It is the identification of person by means of
the ridges of the fingers to include the palms of
the hands and the soles of the feet.
AS AN IMPRESSION
GENERAL GROUPS: SUB-GROUPS:
1. ARCH 1. Plain Arch
2. Tented Arch
2. LOOP
3. Radial Loop
3. WHORL 4. Ulnar Loop
5. Plain Whorl
6. Central Pocket Loop
Whorl
7. Double Loop Whorl
8. Accidental Loop Whorl
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Plain Arch
Tented Arch
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Radial Loop
The type in which the
ridges enter on either side
of the impression recurve,
touch or pass an imaginary
line drawn from delta to
the core, terminate or tend
to terminate on the same
side of the impression
from whence such ridge or
ridges entered. The ridges
flow in the direction of the
radius bone, towards the
thumb finger.
The type
consists of two
separate loop
formations,
with two
separate and
distinct sets of
shoulder with
two deltas.
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“SCIENCE OF FINGERPRINTS”
FINGERPRINT:
• is one of the most positive means of personal identification. It
has been found out that the intricate pattern on the fingers are
permanent, individual and never undergo a natural change,
except in the size of the pattern during the life time of individual.
24
8
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GUIDELINES IN
TAKING STANDARD
FINGERPRINT
FINGERPRINTING
•Is the process of
recording
fingerprint
through the use
of fingerprint ink.
Or capturing
finger-print
images using
electronic device.
26
27
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TYPES OF
FINGERPRINT
IMPRESSIONS
1.Rolled Impression
requires that the
thumb be rolled
towards and other
fingers away from the
center of the subject’s
body.
TYPES OF FINGERPRINT
IMPRESSIONS
2. Plain Impression
LATENT PRINTS
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LATENT PRINTS
WHAT CAUSES
THE MARKINGS?
11
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34
2. Sweat or perspiration.
• COMPOSITION OF THE SWEAT
98.5% to 99.5% is water
0.5% to 1.5% solid matter = 1/3 salt, 2/3
urea, volatile fatty acids, albumin acids,
etc.
• THE COMPOSITION OF SWEAT IS NOT CONSTANT, IT
IS INFLUENCED BY:
Climate.
Nutrition or food that the subject eat.
Impurities on the skin surface
Physical condition of the subject.
3. Colored Substances.
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Things to be considered in
searching for latent prints.
• Precaution is important, do not remove the object from the
original position or touch with your bare fingers anything that requires
fingerprint examination.
• Search of latent prints should be conducted in a
systematic way and intelligent manner.
• Amount and quality of powder to be used. The purpose is
to develop latent prints that can be classified, analyzed and identified.
METHODS OF DEVELOPING
LATENT PRINTS:
• PHYSICAL or MECHANICAL
METHOD
• CHEMICAL METHOD
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43
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46
Packaging tape,
Paper tape,
Cello tape,
Masking tape,
Electrical tape,
Leaves, fruit peeling, etc.
48
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50
17
Firearms
Identification
JOINT FORENSICS TRAINING FOR JUDGES, PROSECUTORS AND PUBLIC
ATTORNEYS
• PNP Crime Lab, Camp Crame, Quezon City
• July 26-28, 2017
7/21/2017
Firearms Identification
Objectives:
The participants will be able to:
Questioned Document
Photography Division
Examination Division
Fingerprint
Identification Division
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Mission:
Functions:
Examination of evidence firearms, bullets, cartridge cases,
pellets, wads, bullet fragments, cartridge components and
related specimen;
Test firing of evidence firearms, to determine functionality and
obtain standard specimen;
Test firing/stencil tracing of all firearms for registration/renewal
of registration;
Experimentation and research;
Encoding of evidence bullets and cartridge cases in the IBIS for
correlation;
Crime scene investigation (SOCO);
Preparation of Laboratory Report, and
Appearance in Court as expert witness
Firearms Identification
A discipline of forensic science which has as its
primary concern to determine if a bullet, cartridge
case or other ammunition component was fired by a
particular firearm.
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Ballistics:
study of projectile in motion - - - -
3
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4
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Factors to be considered:
5
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Bloodstain for
serology and/or DNA
6
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Bore
Inside the Gun
Breechface
Extractor
7
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Class Characteristics
Individual Characteristics
8
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Class Characteristics
Known Source: Markings on bullet
Rifling fired from the barrel
caliber
Caliber
θ
5 – Right twist
Angle of
Twist
Cannelure
Groove width
Landmark Width
Individual Characteristics
Produced by microscopic defects or
imperfections inside the gun:
• Produced incidental to manufacture
• Caused by use and/or damage
9
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Individual Characteristics
Evidence Test
“A matching of toolmarks!”
Documentation/Examination Procedures
10
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Basic equipment…
Firearm
Functionality tests
RA10591
Ammunition
Functionality tests
11
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Preservation/marking of
Evidence
Evidence firearms and related specimen should be properly preserved for future
identification and presentation during the trial of the case. It is imperative that these
physical evidence be kept in suitable evidence room so as to retain their evidentiary value
for effective presentation during the trial of the case in court.
In addition to the markings, fired bullets and cartridge cases should be wrapped in a
clean soft dry paper or cotton and placed separately in self sealing plastic packet or any
similar clean container.
The suspected firearm should be properly wrapped and placed in a container, box or
envelope to avoid contamination. Affix EVIDENCE labels.
The Officer should record all steps taken in preserving evidence for future reference.
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Marking a pistol
On the barrel,
(example: engraved
marked “ABC” 10-14-08)
Slide, and
(example:
engraved
marked
Frame or receiver “ABC” 10-
(example: engraved 14-08
marked “ABC 10-14-08”
Marking a revolver
Evidence Tag
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Upper receiver,
Marking a rifle
(example: engraved Barrel, (example:
marked “ABC” “10- engraved marked
25-04” “ABC” “ 10-25-04”
Lower receiver,
(example:
engraved marked
“ABC” “10-25-
04”
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What is IBIS?
Integrated Ballistics Identification System
1 WORKSTATION
Correlation
Server
COMPARE DIGITAL
SIGNATURES, GENERATE
CANDIDATE LIST
PNP IBISTRAX
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9mmGlock
Dec 2005 to Apr 2008
.38 Cal
9mm Glock
9 mm
9 mm
.45 cal
.45 cal
.38 Cal
40 Shooting Incidents from October 26, 2005
.45 cal
to April, 2008 involving 12 guns
Victims: 93 (40 dead, 34 wounded, 4 raped .45 cal
15 unknown status)
9 mm
Major Achievement
The PNP won the “2012 August Vollmer
Forensic Science Excellence Award” (Category:
“Significant Investigative Value in Major Crime”).
The D, PCRG with the D,CL received the award for the C,
PNP at the 119th IACP Annual Conference on September
30, 2012 at San Diego, California
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Balance is Matter
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The PNP shall place this information, including its individual or peculiar
identifying characteristics into the database of integrated firearms
identification system of the PNP Crime Laboratory for future use and
identification of a particular firearm.
20
FORENSIC
MEDICINE
Philippine National Police Crime Laboratory
Medico-Legal Division
FORENSIC
MEDICINE
Philippine National Police Crime Laboratory
Medico-Legal Division
BACKGROUND
The Medico-Legal Division gives
assistance to PNP Investigators as well as to
other government and non-government
investigative agencies through the conduct of
autopsy, physical examination and other relevant
forensic examination. Further, this division
provides quality scientific investigation and works
in partnership with responsive pillars of the
criminal justice system towards the attainment of
equality and justice.
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Forensic Medicine
• Deals with the interaction of medical science with
the law.
• Forum
• All branches of medicine can have forensic aspect.
• The main objective is the TRUTH based on the
evidence.
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Two types:
1. Primary: Scientific
•Fingerprint identification
•Dental identification
•DNA identification
2. Secondary:
•Tattoos
•Scar
Post-mortem fingerprint
The result was completed in less than an hour that
yielded to the positive identification of the owner of
the fingerprints.
Telesporo G Pacampara
Case: Carnapping
Dental Identification
Julia Campbell ?
3
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DNA EVIDENCE
4
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Time of Death
5
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LIVOR MORTIS
•Reddish, purplish blue
color
•Begins in 30 mins
•Intensifies over time
•May result in post
mortem petechiae
•Its not a contusion
•Livor vs contusion?
RIGOR MORTIS
• depletion of ATP
•Begins in 2 hrs
•Jaw, face, arms, legs
•Full rigor in 6-12 hrs
•Lost due to
decomposition
•Usually disappear
after 24 hrs
•Cadaveric spasm-rare
BODY TEMPERATURE
•35.6°C - 38.2°C
•Higher in girls
•Infants cool faster
•Obese cools at lower rate
•Not accurate
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STOMACH CONTENTS
•Gastric emptying
varies from person to
person, amount of
meal and time of the
day
•Half gastric emptying
time: 4 hrs
•Stress will delay
digestion
ENVIRONMENTAL FACTORS
DECOMPOSITION
• Autolysis: - aseptic breakdown
- pancreas
• Putrefaction: breakdown due to bacteria
- 1st sign is greenish color of LQ abdomen
- greenish-black color of face/neck
- swelling
- protruded eyes and tongue
- purge fluid
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Cont: decomposition
• Gas formation- slippage of skin with blister and
marbling ( rxn of hgb and hydrogen sulfide). Skin
from green to black.
• Hair will slip from the scalp
• brain: liquefied
• Decomposition: after 24hrs in hot weather
• Skeletonization: a week to years
• Adipocere: fats will undergo fatty acid
transformation
MEDICO-LEGAL DEATHS
1. VIOLENT DEATHS - accidents
- suicides
- homicides
2. SUSPICIOUS DEATHS
3. SUDDEN UNEXPECTED DEATHS / MEDICALLY
UNEXPLAINED DEATHS
4. UNATTENDED DEATHS
MEDICO-LEGAL DEATHS
5. DEATH IN CUSTODY
6. POISONING
7. POSSIBLE THREAT TO PUBLIC HEALTH
8. DEATH RELATED TO EMPLOYMENT
9. DEATH ASSOCIATED WITH THERAPEUTIC AND
DIAGNOSTIC PROCEDURE
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MEDICO-LEGAL DEATHS
10. UNLAWFUL TERMINATION OF PREGNANCY
11. BODIES TO BE CREMATED
12. UNCLAIMED CADAVERS
13. SUSPICIOUS CHILD DEATHS
14. UNEXPECTED DEATH OF PUBLIC OFFICIAL
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GUNSHOT
Cause of death
WOUND
causes of death
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DEATH CERTIFICATE
• To consider or probable cause of death is accepted.
• Time of death, always include.
• Deaths other than natural, FOR AUTOPSY.
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Autopsy
• A comprehensive study of a dead body, performed by a trained
physician.
• When shall an Autopsy be performed on a dead body:
PD 856 Code on Sanitation, Section 95
• Whenever required by special law
• Upon order of a competent court, a mayor, a provincial or city
fiscal
• Upon written request of police authorities
• Whenever the Solicitor General, provincial or city fiscal as
authorized by existing laws, shall deem it necessary disinter and
take possession of remains for examination to determine the
cause of death; and
• Whenever the nearest kin shall request in writing the authorities
concerned to ascertain the cause of death
1. Health officers;
2. Medical officers of law enforcement
agencies; and
3. Members of the medical staff of accredited
hospitals.
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AUTOPSY REPORT
1. External Description. Photo, fingerprint
2. Evidence of Medical / Surgical Intervention
3. Evidence of Injury: recent, external, internal.
a. GSW:
b. SW:
c. Clothing:
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AUTOPSY REPORT
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Revolver
•A revolver has
cylindrical
magazine which
holds six rounds of
cartridges.
•Muzzle velocity:
600 feet per second
Automatic Pistol
•Auto loading pistol.
•The detachable
vertical magazine
holds 17 rounds.
•Muzzle velocity:
1200 feet or more
per second
Rifle
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Shotgun
GSW
ENTRANCE:
A. Contact Fire
- circular / stellate
- abrasion collar is distinct
- muzzle imprint
- smudging, burning or tattooing are prominent
with singeing of hair
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Contusion collar
Muzzle imprint
Entrance site
• When a bullet enters the
skull it produces a sharp-
edged "punched-out"
hole in the outer table,
with a larger
corresponding "beveled-
out" hole on the inner
table
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Smoke smudging
Soot
• The surface of the skull
demonstrates the heavy
soot in this contact range
entrance wound, as well
as radiating fracture
lines. The direction of fire
was thus toward the
back of this picture.
GSW
ENTRANCE:
B. Close range
- 1 to 15 cm
- powder burns / tattooing
- smudging
- singeing/ hair burning
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Gunpowder Tattooing
• This is an intermediate
range gunshot entrance
wound in which there is
powder "tattooing"
around the entrance site.
Gunpowder Tattooing
• Powder tattooing is seen
in this intermediate
range gunshot wound.
The actual entrance site
is somewhat irregular,
because the bullet can
tumble in flight.
ENTRANCE
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GSW
ENTRANCE:
D. Medium Range
- more than 15cm but les than 60cm
- gunshot wound is circular or oval,
- inverted edged
- tattooing present but of lesser density
- smudging is present if less than 30cm
- abrasion collar present
20
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GSW
ENTRANCE:
D. Longer Range
- more than 60cm
- gunshot wound is circular or oval
- no powder burns / tattooing
- no smudging
- no singeing/ hair burning
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Microscopic Exam
• Histologic examination of
the entrance wound site
on the skin demonstrates
black gunshot residue
and coagulative necrosis.
(+) Abrasion Collar (+) Abrasion Collar (+) Abrasion Collar (+) Abrasion Collar
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GSW
EXIT WOUNDS:
1. Everted
2. Stellate
3. No burning, tattooing
4. Varied shapes
5. Sometimes more than one exits
Exit site
• As the bullet exits the
cranial cavity, the inner
table appears "punched-
out" with beveling on the
outer table
Exit Wound
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Exit wound
• Here is a slit-like exit
wound. The projectile
became deformed and
flattened while traversing
the body, producing a
laceration upon exit.
Note that there is no
powder or soot visible in
this exit wound.
Keyhole wound
• Tangential source
• One end of the
perforation will resemble
a typical entrance defect,
while the other end will
show external beveling
consistent with exit holes
24
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POX
POE POX
SHOTGUN
Close range,
Pellets and wad recovered
showing smudging
25
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Fracture
Shot gun
Shot gun
GSW
Grazed wound
26
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Grazed wound
“Raccoon eyes”
Fragmented FMJ
27
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Gunshotwound
GSW
SUICIDE or HOMICIDE:
1. GSW is arm’s reach
2. Weapon should be present
3. Usually the mouth, temple, anterior neck
chest.
4. Not in the eye, abdomen or back
5. Woman rarely use gun.
6. Multiple GSW suggest homicide.
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GSW
DOCTORS DUTY:
1. Take pictures before treatment.
2. Take notes, measure and draw.
3. Preserve the evidence collected.
4. Call the police.
5. All deaths are medico-legal case.
1. Flame
2. Contact
3. Radiant heat
4. Scalding
5. Chemical
6. Microwave
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Burns
33
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Rule of nine
Electrocution
34
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Death by Fire
Questions
to address:
1. WHO IS IT?
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During Autopsy
•External examination
• may reveal bright red lividity
• pugilistic posture
•Internal examination
• bright red discoloration of tissues and the presence of
soot and/or scorching of the airways may be evident
• extensive postmortem burning, internal organs can
become heat-fixed, or cooked.
•Artifacts
• skin splitting.
• long bones that appear
to be fractured.
Artifacts
• Heat-induced skull fractures
• skull fracture/epidural hematoma complex is
considered a classic heat induced artifact
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Mechanism of Death
•Chemical asphyxia due to carbon monoxide
unconsciousness
Physical injuries
CLASSIFICATION OF WOUNDS
1. ABRASIONS
2. CONTUSIONS
3. HEMATOMA
4. LACERATIONS
5. STAB WOUNDS
6. INCISED WOUND
7. GUN SHOT WOUNDS
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ABRASIONS
CONTUSIONS
HEMATOMA
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LACERATIONS
STAB WOUNDS
INCISED WOUND
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INCISED WOUND
• Location, important
• Is it a defense wound?
CHOP WOUNDS
INCISED WOUNDS
SUICIDE:
1. The hallmark of self infliction is repetition.
2. Usually grouped incisions, parallel
3. Usual site: throat and wrist.
4. trial or tentative cuts
5. Homicidal cut throat is devoid of tentative cuts
and is severe.
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ELECTROCUTION
SEXUAL CRIMES
42
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ANATOMY
FEMALE GENITALIA
ANATOMY
MALE GENITALIA
TYPES OF HYMEN
43
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CRECENTRIC
ANNULAR
SEPTATE
Other Variants
44
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SEXUAL ABUSE
SEXUAL ABUSE
45
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SEXUAL ABUSE
SEXUAL ABUSE
Medical evaluation shows definite evidence of
sexual abuse or (RECENT) sexual contact.
Criteria:
1. Positive for sperm or seminal fluid.
2. Pregnancy
3. Gonorrhea, Syphilis, Trichomonas,
Chlamydia and HIV
SEXUAL ABUSE
Ano-genital findings are diagnostic of
recent/previous blunt force or penetrating
trauma.
Criteria:
1. Acute hymenal injury-partial or complete
2. Perianal lacerations
3. Healed hymenal lacerations
4. Acute injuries on the labia, fourchette.
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47
Forensic DNA Testing
Basics
PNPCL DNA ANALYSIS BRANCH
Learning Goal
PNPDNA 2
Training Objectives
PNPDNA 3
1
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Definition of Terms:
DNA
The abbreviation for deoxyribonucleic acid, which is the genetic material
present in the cells of all living organisms. DNA is fundamental building
block for an individual’s entire genetic make-up.
Buccal
Inside cheek, mouth cavity, or oral.
CODIS
Combined DNA Index System. DNA database software shared by United
States Federal Bureau of Investigation.
PNPDNA 4
Functions:
Collect
Preserve
Secure all
evidence and
specimen
submitted
Ensure they are
free from any
contamination
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DNA and
fingerprint
analyses are
similar in how
matching is done.
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Criminal Cases
• Free of charge, except for VAWC cases
claiming support
• Casework
• Databasing
PNPDNA 11
4
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PNPDNA 13
PNPDNA 14
PNPDNA 15
5
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PNPDNA 16
PNPDNA 17
PNPDNA 18
6
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PNPDNA 19
PNPDNA 20
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STR Workflow
Screening
• Examination of
evidence for
biological stains
• Documentation of
evidence
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STR Workflow
Serology
• the use of chemicals to
confirm a substance is
biological in nature
and/or determine the
type of biological
material (i.e., semen,
blood, saliva, etc.)
PNPDNA 24
8
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STR Workflow
Sampling
• the use of chemicals to
confirm a substance is
biological in nature
and/or determine the
type of biological
material (i.e., semen,
blood, saliva, etc.)
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STR Workflow
DNA Extraction
• Releases DNA from the
cell
• Isolates DNA from other
cellular components
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STR Workflow
STR Amplification (PCR)
• Generates millions of
copies of the genetic
markers of interest using
fluorescent dyes and
commercial STR primers
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STR Workflow
Capillary Electrophoresis
• Capillary electrophoresis generates
the data necessary to determine
fragment size and associated dye
color
– DNA carries a constant negative
charge
– DNA migrates toward the positive
charge when current is applied
– Smaller DNA molecules migrate
faster
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11
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Releasing of Report
• Prior to the release of a
DNA laboratory report, an
administrative and
technical review must be
done by:
1. colleague DNA analyst
2. Chief, DNA Analysis
Branch
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Legal Issues
PNPDNA 36
12
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Summary
• DNA is a powerful tool used to identify individuals
and provide investigative leads in criminal
investigations to mass disasters
• Unique DNA profiles can be obtained from various
types of biological testing using STR genetic markers
• Once the DNA is processed in the lab, the DNA
profiles from crime scenes are compared against
reference profiles and profiles stored in DNA
databases with the goal of finding a matc
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Questions?
PNPDNA 42
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