UREA KIT (Mod. Berthelot method) Fertoegaleminton of Ure in sarun plasma and une, {Forte Diagnostic Use Ory), Summary Ura is the en product of rosin metabolism. His _yntesned nto er tom be ammonia produced byte feraols of amino aces. transported by he lo fo the Woy om where i exceed. Increased eels are font in ronal csoeses, inary cbeuctons, shack engstve nea fle and burs, Decreased loves as {oxnsin ioral arepeognaney Prine Ursese hele rats ammonia and CO, The snmonia ored fuer reacts wih a phenoic chogen and pesto form a roe cold coal norsty of the colour formats cect proporiens! tothe amount of UWeapeeenintesenple vero amen, “tote Normaraerence values Sorum/Piasne ine 14 samgis Uptozagi, Its recommendes tat each aboratory etabish is oun rralrange representing spate pzplalon. Beet EST Soa A stage ey Conn etal al 24°C ih xoiy marten thelabels Reagent Proparaton Reagersareroady tous forte gen procedure Working Enzyme Reagent : For the foxblty and onvenienc in petorming lage assy srs, a working emzyereapeimayb mateby posing btieof12 (Enmyme Roagen int bole ot 1 (Bur Reger) For smalr series conbino 10. pats of L1 (Bur Reagent) and par of L2 ErayneReagen) Use tm! fhe wetrg 9apen or aray sags ff Lt ad Us mf 2. a gvan in the procedure The woking snzymereapert isso alleasl4 wooks whe ord 28 LT Working Chromogen Reagent : For larger voume tes, dt + pat of (Ctrmogan Reagent wih 4 ars of tes armaria e dibled/donsa8 water Use ‘imlot waiegcromogon instead of2lin te asa. ‘The working ehoagen reagent i sabi or aleast 8 weeks when sored at 28°C in ghlysloppered patic bate, Sample mateal Serum, plasma, Une Ole rin 1 4 wih dst ator afore tha assay Resuls 50), Urea sepa to be stab in he serum fo 5 days whan sored at 28°C, Procedure Wile er 570m (Hg $78) Yebow Terpeaire STIR. Lint path ton pate ino dean yet tubes abla as Blak), Sand) an Test (7) ‘een 8 7 Seance io ou Bair Roogemy) | 10 10 Eemmmmgenilz) | 01 oft Dated tr oot sole aot Ts of PR oO OR RT OD ChonegerResweris] 02 tz 02 ax wal and incite for min. 37°C TO min. 8 RT. (25) Measure tne aoeotance of fe Sandra ats 8), andTest Sample ADs T) agate Bian, wiring 2 a Linearity “This pocedre is Incr up 252 mai. Using te working chromogen eagent (the neat sizes to 400 raid values enceedtiiit dts ha sarumit Notes ‘ry cotarinlen by amon or arsrarum sad ‘eongous resus nes asa should rot be coectd wit Fei orHeparn Ammonis sas, “The woreng enzyme reagan! rl ile ot eleva emperatures and. should be stored at 28°C crosses “The chromogen reagent contin chair. Te bots shoud be opened ony when requred ad closed hy _aruseo provost ss ofacve chore.References Beret MLPE, (1859) Reno Chin. App 2884 Fawoet JK, Set, 1660). Chin Pal 19196 ‘System Parameters Reaction Ena Pont Wavelangh 570m Zero Seting : Reagent Banc Incub. Temp, STCIRT Inoub. Time Sin +S. oO ‘amin + 10min, Delay Tine ReadTime : = Nocofread : = Inter 7 Sample Vol: 001ml Reagent Vol. : 130m Standard} Omg Factor — React Slope nseasng Uneariy”: 250 rgd Unis rat remo, tana Or Artenio De Rego Bagh, Bamboim Complex 2.0, GOA. 408 202, NDIA