Lampiran 1.
Prosedur Pemeriksaan Kolesterol Total
Metode
+ Bnzimatikfotometer CHOD PAP
‘Tujuan + Mengetahui kadar kolesterol pasien
Prinsip Penentuan kolesterol setelah hidrolisis enzimatk dan
coksidasi. Indikator wama yaitu quinonemine yang
ihasitkan dari 4-aminoantpirin dan fenol dalam
hhidrogen peroksida di bawah katalis peroksidase.
Bahan + Serum, reagen kontrol, reagen blank, aquades.
Carakerja =
1) Siapkan alat dan bahan yang akan digunakan
2 Diletakkan masing-masing tiga tabung reaksi pada rak tabung
13) Piet 10001 reagen menggunakan mikropipet, masukan ke del ‘masing-
masing tabung.
4) Pada tabung blanko ditambahkan 10 pl aquadest, pada tabung standar
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