Volume Enzim Volume Konsentrasi OD kontrol OD sampel Kecepatan

(ml) Akuades (ml) Sampel (%) katalisa enzim

0.1 0.1 0.001 0.689 0.653 52.25

0.2 0.2 0.002 0.639 0.646 -10.95

0.3 0.3 0.003 0.686 0.596 131.195

04 04 0.004 0.677 0.594 122.6

0.5 0.5 0.005 0.718 0.593 174.09

0.6 0.6 0.006 0.732 0.583 203.55

0.7 0.7 0.007 0.761 0.559 265.44

0.8 0.8 0.008 0.741 0.543 267.21

0.9 0.9 0.009 0.720 0.542 247.22

1.0 1.0 0.01 0.725 0.518 285.52

 Pengaruh Konsentrasi Enzim terhadap Kecepatan Reaksi Enzim

350
y = 26.738x + 43.226
300
keceptan Katalisa Enzim

R² = 0.9398

250

200

150

100

50

0
0 2 4 6 8 10 12
Konsentrasi Enzim

(𝑂𝐷 𝑘𝑜𝑛𝑡𝑟𝑜𝑙 𝑥 𝑃)−(𝑂𝐷 𝑆𝑎𝑚𝑝𝑒𝑙 𝑥 𝑃)

Kecepatan katalisa enzim (% substrat pati terhidrolisa) = . 100%
𝑂𝐷 𝑘𝑜𝑛𝑡𝑟𝑜𝑙

(0.689 𝑥 10)−(0.653 𝑥 10)

Tabung 1 = . 100%
0.689

= 52.25 % substrat pati terhidrolisa

(0.639 𝑥 10)−(0.646 𝑥 10)
Tabung 2 = . 100%
0.639

= -10.95 % substrat pati terhidrolisa

(0.686 𝑥 10)−(0.596 𝑥 10)
Tabung 3 = . 100%
0.686

= 131.195 % substrat pati terhidrolisa

(0.677 𝑥 10)−(0.594 𝑥 10)
Tabung 4 = . 100%
0.677

= 122.6 % substrat pati terhidrolisa

(0.718 𝑥 10)−(0.593 𝑥 10)
Tabung 5 = . 100%
0.718

= 174.09 % substrat pati terhidrolisa

 (0.732 𝑥 10)−(0.583 𝑥 10)
Tabung 6 = . 100%
0.732

= 203.55 % substrat pati terhidrolisa

(0.761 𝑥 10)−(0.559 𝑥 10)
Tabung 7 = . 100%
0.761

= 265.44 % substrat pati terhidrolisa

(0.741 𝑥 10)−(0.543 𝑥 10)
Tabung 8 = . 100%
0.741

= 267.21 % substrat pati terhidrolisa

(0.720 𝑥 10)−(0.542 𝑥 10)
Tabung 9 = . 100%
0.720

= 247.22 % substrat pati terhidrolisa

(0.725 𝑥 10)−(0.518 𝑥 10)
Tabung 10 = . 100%
0.725

= 285.52 % substrat pati terhidrolisa