a, Ribbon representations of the profilin–actin complexes.

(Kaiser & Bond, 1959) The overlays were

created by superimposing the actin structures to show the relative binding geometries of the profilins
(abbreviated as P).(motivation, n.d.) For clarity, only one actin is displayed. The positions of Loki profilin -
1 and Loki profilin-2 overlay well with the major interaction with subdomain 3. The rabbit α-actin
subdomains are indicated as black numbers.(Mita, 1996) Some flexibility is observed in the binding mode
of Loki profilin-2, as seen by a slight shift between the two complexes in the asymmetric unit (labelled
LokiP2A and LokiP2B). Human profilin-1 and Odin profilin adopt binding positions that also interact with
subdomain 3, but which additionally have more-intimate contacts with rabbit α-actin subdomain 1.(Geis,
Twichell, Efremow, Krohn, & Lyszczarz, 1996) The human profilin-1–rabbit α-actin and Odin profilin–
rabbit α-actin complexes are shown separately to aid in interpretation of the overlay. Bound calcium ions
are shown as black spheres and ATP in stick representation. b, Latrunculin B (LatB, cyan) binds to
analogous positions in rabbit α-actin in each complex. The Odin profilin–rabbit α-actin binds to a second
molecule of latrunculin B at the edge of the Odin profilin–rabbit α-actin interface. For clarity, two views of
Odin profilin–rabbit α-actin are shown. c–f, Cartoons of the profilin structures rotated by 180° around
the yaxis with respect to Fig. 1b, d, e, f. g–i, Cα traces of the superimpositions of human, Odin, Loki
profilin-1 and Loki profilin-2 structures (g), Loki profilin-1 and Loki profilin-2 structures (h) and the
human and Odin profilins (i) to highlight their similarities and differences. j–l, The 2Fo−Fc electron density
contoured at 1.0σ—indicating the quality of the structures—is shown surrounding the Loki-loop regions of
Loki profilin-1 (j) and Loki profilin-2 (k), along with the absence of the Loki-loop in this region in Odin
profilin (l).

References
Geis, M. W., Twichell, J. C., Efremow, N. N., Krohn, K., & Lyszczarz, T. M. (1996). Comparison of electric field
emission from nitrogen‐doped, type Ib diamond, and boron‐doped diamond. Applied Physics Letters, 68(16),
2294–2296. https://doi.org/10.1063/1.116168
Kaiser, W., & Bond, W. L. (1959). Nitrogen, A Major Impurity in Common Type I Diamond. Physical Review,
115(4), 857–863. https://doi.org/10.1103/PhysRev.115.857
Mita, Y. (1996). Change of absorption spectra in type-I b diamond with heavy neutron irradiation. Physical Review
B, 53(17), 11360–11364. https://doi.org/10.1103/PhysRevB.53.11360
motivation. (n.d.).