FASTA:

>KY244026.1 Triticum aestivum cultivar HD 2967 nitrate reductase (NR) mRNA,

partial cds
GGTTCCTTCCTTTCCTCTTCTGCGGCGACCAATCCAGAAACCATCCAACGCTTGTCCCACGACGCGGCAC
AGCGCACCATGGCTGCTTCGGTCGAGCCACGGCAGTCGTCGTTCGGCCGCCTCGACGCGCCGGCCACCGC
GCCGCCGGCACGCAACGGTTCCTCCAACGGCGGCATCCGTCGCCGCGCCGACTCGCCGGTGCGCGGCTGC
GGGTTCCCCCCGCTCATTTCGCCGCCGCGGAAGGCCATCGTCGAGGAAGAGGAGCAGGACGAGGAGGACG
AGGAGCCGGAGGAGGACTGGCGGGAGGCGTACGGCTCGCAGCTGCAGCTGGAGGTGGAGCCGTCGACGCG
CGACCCGCGGGACGAGGGCACGGCGGACGCCTGGATCGACCGCAACCCCTCGCTGATCCGCCTCACCGGC
AAGCACCCGCTCAACTGCGAGCCGCCGCTGGCGCGCCTCATGCACCACGGCTTCATCACGCCCGCGCCGC
TCCACTACGTGCGCAACCACGGCGCCGTGCCAAAGGGCGACTGGGCCACCTGGACGGTCGAGGTGACAGG
GCTCGTCCGCCGCCCCGCGCGGCTCACCATGGACGAGCTGGCCAACGGGTTCGCCGCCGCGGAGGTGCCC
GCCACGCTGGTGTGCGCCGGCAACCGCCGCAAGGAGCAGAACATGGTGCAGCAGACGGTGGGCTTCAACT
GGGGCGCGGCGGGGGTGTCCACCTCCGTCTGGCGCGGGGCGCGCCTCCGCGAAGTGCTGCTCCGGTGCGG
CATCATGTCGCGCAAGGGGCAGGCCCTGAACGTGTGCTTCGAGGGCGCCGAGGACCTCCCCGGCGGCGGC
GGCTCCAAGTACGGCACCAGCGTGACCCGGGAGTGGGCGCTCGACCCGTCCCGCGACATCCTGCTCGCCT
ACGCGCAGAACGGCGAGCCGCTGCTGCCCGACCACGGGTTCCCGGTGCGCGTGCTCATCCCCGGCTGCAT
CGGCGGCCGCATGGTCAAGTGGCTCCGGCGCATCGTCGTCACCCCCGCCGAGTCCGACAACTACTACCAC
TTCAAGGACAACCGGGTGCTGCCGTCGCACGTGGATGCGGAGCTGGCCAACGCGGAGGCGTGGTGGTACC
GGCCCGAGTACATCATCAACGAGCTCAACACCAACTCGGTGATCACCACGCCCGGGCACGACGAGATCCT
CCCCATCAACGCCTTCACCACCCAGCGCGCATACACCATGAAAGGATACGCATACTCCGGTGGTGGTAAG
AAGATCACACGAGTGGAAGTGACTCTGGACGGCGGCGAGAGCTGGATGTTGTGCACGCTCGACATCCCGG
AGAAGCCGAACAAGTACGGCCGGTACTGGTGCTGGTGCTTCTGGTCGGTGGAGATCGAGGTGCTGGACCT
CCTTGGGGCCAAGGAGGTGGCGGTGCGCGCGTGGGACCAGACCCACAACACCCAGCCGGAGAAGCTCATC
TGGAACCTCATGGGCATGATGAACAACTGCTGGT

Primers: start len tm gc% any 3' seq

LEFT PRIMER 949 20 57.98 55.00 5.00 3.00 GCCGAGTCCGACAACTACTA

RIGHT PRIMER 1403 20 56.33 45.00 4.00 2.00 ATGAGGTTCCAGATGAGCTT

PRODUCT SIZE: 455, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00