9.

why direct coomb’s test include in this

Answer

The coomb’s test (the anti globulin test) was introduced by coombs, Mourant and Race in 1945. The
was developed to detect antibodies which can bind the antigen on the surface of RBC’s but cannot
agglutination them (incomplete antibodies).

To identifity if a patient’s RBC’s are coated with immunoglobulin complement or both. AHG with
reactivity to human immunoglobulin and / or compliment is added to the patient’s RBC’s. If cross
linking and subsequent agglutination is present,direct coomb’s test is positive

Useful for the detection of immunoglobulin (IgG) or complement adsorbed into red cells. Used to
detect autoimmune hemolytic anemias causwd by antibody and/or complement components being
bound to the patient’s red cells (including drug induced),transfusion reaction and erythroblastosis
fetalis (hemolitic disease of the newborn). Coted red cells are said to be “sensitized”.

In a DAT (Direct Antiglobulin/Coombs Test), the test is for antibody atteched to the pattient’s red
cells in vivo. In an Antibody Screen (Indirect Antiglobulin Test),the antigen-antibody reaction accurs
in vitro and one tests patients serum for antibody with reagen t cell (the antigen). Refer to Direct
Coombs/Antibody Screen or Type and Direct Coombs for alternative test combination