Metode u DNA tehnologijama

Domaća zadaća 1: Pronalazak i analiza sekvence gena- baza podataka Ensembl

Ime i prezime studenta:

Akad. godina i smjer: 1.godina, Istraživanje i razvoj lijekova
Gen: TSPAN11
Organizam: čovjek

DIO 1: ANALIZA GENA

1. Na kojem kromosomu se nalazi gen? 12
2. Na kojem kraku kromosoma? p
3. Koliko je velik gen? 70171 pb
4. Koliko transkripata postoji? 4
5. Koliko transkripata kodira protein? 4
6. Koji transkript(i) kodira(ju) najduži protein? TSPAN11-001 i TSPAN11-002

DIO 2: ANALIZA TRANSKRIPTA

7. Navedite naziv analiziranog transkripta. TSPAN11-001
8. Koliko egzona ima navedeni transkript? 8
9. Koliko introna ima navedeni transkript? 7
10. Koji je najveći egzon i kolika mu je veličina? Egzon 8 (ENSE00002241795) veličine 296 pb
11. Koji je najveći intron i kolika mu je veličina? Intron 1-2 veličine 27184 pb
12. U kojem egzonu se nalazi prvi metionin? 2
13. Koliko je velika 5' UTR regija? 60 pb
14. Koliko je velika 3' UTR regija? 236 pb

Domaća zadaća 1 1
 Metode u DNA tehnologijama

DIO 3. PORAVNANJE SEKVENCI GENA ČOVJEKA I MIŠA

Rezultat poravnanja kodirajućih sekvenci čovjeka i miša.

CLUSTAL O(1.2.4) multiple sequence alignment

hTSPAN11-001 ATGGCCCACTATAAGACTGAGCAGGACGACTGGCTGATCATCTACTTGAAGTATTTACTC
mTspan11-001 ATGGCGCACTGCAAGACAGAGCAGGATGACTGGCTGCTGGCCCACCTGAAGTACCTGCTC
***** ****. *****:******** *********.* . * ** ******* *.***

hTSPAN11-001 TTTGTCTTCAACTTCTTCTTCTGGGTCGGGGGAGCAGCCGTCCTGGCTGTGGGCATCTGG
mTspan11-001 TTCATCTTCAACTTCTTCTTCTGGGTAGGTGGGGCAGCCGTCATGGCCGTGGGTATCTGG
** .**********************.** **.*********.**** ***** ******

hTSPAN11-001 ACCCTGGTGGAGAAGAGTGGCTACCTCAGCGTCCTGGCCTCCAGCACCTTTGCCGCCTCC
mTspan11-001 ACACTGGTGGAGAAGAGCGGTTACCTCAGCATCTTGGCCTCCAGCACGTTCGCTGCCTCT
**.************** ** *********.** ************* ** ** *****

hTSPAN11-001 GCCTACATCCTCATCTTTGCGGGCGTACTTGTCATGGTGACCGGCTTCCTGGGCTTCGGT
mTspan11-001 GCCTACATCCTCATTTTCGTTGGTGGTCTTGTCATGACAACTGGCTTCCTGGGCTTTGGG
************** ** * ** * :*********. .** ************** **

hTSPAN11-001 GCCATCCTCTGGGAGCGGAAGGGCTGCCTCTCCACGTATTTCTGCCTGTTGCTCGTCATC
mTspan11-001 GCCATCATCCGGGAGCAGAAGAGCTGCCTCTCCACATATTTCTGCCTGCTGCTGGTCATC
******.** ******.****.*************.************ **** ******

hTSPAN11-001 TTCCTGGTTGAGCTGGTGGCGGGAGTCCTGGCCCATGTGTATTACCAGAGGCTGAGTGAT
mTspan11-001 TTCCTTGTGGAGCTGGTGGCAGGGGTCCTGGCACATGTGTACTACCAGAGGCTGAGTGAC
***** ** ***********.**.********.******** *****************

hTSPAN11-001 GAACTGAAGCAGCACTTGAACCGGACTCTGGCTGAGAACTACGGGCAGCCCGGAGCCACG
mTspan11-001 GAGCTGAAGTGGCACCTGAACAGCACCCTGACTGAGCACTACGGACAGCCCAGGGCCGCA
**.****** .**** *****.* ** ***.*****.*******.******.*.***.*.

hTSPAN11-001 CAGATCACCGCCTCAGTGGACCGACTCCAGCAGGATTTCAAGTGCTGTGGAAGCAACAGC
mTspan11-001 GAGATCACAGCCTCGGTGGACCGGCTGCAGCAGGACTTCAAATGCTGTGGCAGTAACAGC
*******.*****.********.** ******** *****.********.** ******

hTSPAN11-001 TCAGCCGACTGGCAGCACAGCACGTACATCCTGTTGCGGGAGGCCGAGGGCCGCCAGGTG
mTspan11-001 TCAGCCGACTGGCAGCACAGCGCGTACATCTTGTCTCAGGAAGCCCTGGGTCGCCAGGTG
*********************.******** *** *.***.*** :*** *********

hTSPAN11-001 CCCGACAGCTGCTGCAAGACAGTGGTGGTGCGCTGCGGCCAGCGGGCCCACCCCTCCAAC
mTspan11-001 CCCGACAGCTGCTGCAAGACAGTGGTGGCCCGCTGTGGTCAGCGAGCCCACCCCTCCAAC
**************************** ***** ** *****.***************

hTSPAN11-001 ATCTATAAGGTGGAGGGAGGCTGCCTCACCAAGCTGGAGCAGTTCCTGGCCGACCACCTG
mTspan11-001 ATCTACAAGGTAGAGGGAGGCTGTATGGCCAAGCTGGAACAGTTCGTGGCTGACCACCTG
***** *****.*********** .* .**********.****** **** *********

hTSPAN11-001 CTGCTTATGGGGGCAGTGGGCATCGGGGTGGCCTGCCTGCAGATCTGCGGGATGGTTCTC
mTspan11-001 CTGCTCATGGGAGCGGTGGGTATTGGGGTAGCCTGTCTTCAGATCTGTGGGATGGTCCTC
***** *****.**.***** ** *****.***** ** ******** ******** ***

hTSPAN11-001 ACCTGCTGCTTGCACCAGAGGCTCCAGCGGCATTTTTACTAA
mTspan11-001 ACCTGCTGCTTGCACCGAAGGCTCCAGCAACAATTTTATTAA
****************..**********..**:***** ***

Domaća zadaća 1 2
 Metode u DNA tehnologijama

Rezultat poravnanja aminokiselinskih sekvenci čovjeka i miša.

CLUSTAL O(1.2.4) multiple sequence alignment

hTSPAN11-001 MAHYKTEQDDWLIIYLKYLLFVFNFFFWVGGAAVLAVGIWTLVEKSGYLSVLASSTFAAS
mTspan11-001 MAHCKTEQDDWLLAHLKYLLFIFNFFFWVGGAAVMAVGIWTLVEKSGYLSILASSTFAAS
*** ********: :******:************:***************:*********

hTSPAN11-001 AYILIFAGVLVMVTGFLGFGAILWERKGCLSTYFCLLLVIFLVELVAGVLAHVYYQRLSD
mTspan11-001 AYILIFVGGLVMTTGFLGFGAIIREQKSCLSTYFCLLLVIFLVELVAGVLAHVYYQRLSD
******.* ***.*********: *:*.********************************

hTSPAN11-001 ELKQHLNRTLAENYGQPGATQITASVDRLQQDFKCCGSNSSADWQHSTYILLREAEGRQV
mTspan11-001 ELKWHLNSTLTEHYGQPRAAEITASVDRLQQDFKCCGSNSSADWQHSAYILSQEALGRQV
*** *** **:*:**** *::**************************:*** :** ****

hTSPAN11-001 PDSCCKTVVVRCGQRAHPSNIYKVEGGCLTKLEQFLADHLLLMGAVGIGVACLQICGMVL
mTspan11-001 PDSCCKTVVARCGQRAHPSNIYKVEGGCMAKLEQFVADHLLLMGAVGIGVACLQICGMVL
*********.******************::*****:************************

hTSPAN11-001 TCCLHQRLQRHFY
mTspan11-001 TCCLHRRLQQQFY
*****:***::**

Domaća zadaća 1 3