‘ETiaINor TlpoooikHE ANAKOINOZHE Mopopohoyixit kat avoooiotoxnquxn jieAétn tou pvoKapsiou Tetpapar{aav ped tm Yopttynon weotoowepovns 2s Tlanaptroov ©, Aeprerifonotiov-Ceo8apifov M., Muaphayidvrns A., Karotfa A., Koravidou E., Mdvios A. Nepikhyh H reotootepéin evar 10 xipio avBpoyvo ae dpeva ahd xa Oitiea drowa Kat a€ ovvBuaou ste ov HeraBonien img 6ei6poreotoorepdvn aoxet avaBohixéc Ka avbipoyorKé emépdoerc Siapéoou tov unoboxéa tov avépoys ov anid xar péow pn youBiaxv Spspiov (non genomic effects). Meydho evbapépov undpxer otn piBhioypa- fa oxeuxd ye 1a anotehéopaca tne e€ayevouig xopriynane avapohukcr-awépoySvav atepoetSisv ato j1v0K4p510, Tous axeheukodc peg, 10 fap Kar dAAoUs ratoKe. Or HEXp! tsipa avaKoIoErG rapovoKAzoUY erepoyévera Ox zpos inv apélypn y1a to pruoxdip6i0 fn eniSpaon wav avopokixsy avSpoycva orepoeiBiv. Sxorée tc napob- ac pedéme trav va xataGerxOouv oF unepuNKpoOKOMKG en{neG0 o1 athoIioeIC MOU TUX6Y MpoxahowvTa! O10 _nvoxdp610 tou erituog ané tn xopmynon teovoorepding oe auvBvaa}6 pe tv avoooioroxnpunt Btepedvnon tg _netaBottic inc éegoaone tov avépoyornsot umoSoxéa. _XpnoiyionostiOnxav Aeuxol dppevec eviiduxee emiuvec tng puluic Wistar ot omofo1 xepto8nKav oe Bv0 opdbec. H pian opc6a anoredetto and enfues o1oue onofoug xopnynGnkav 12mg teorootepdine evGoqviKG y1a efkoo! (20) ouvexele nrépec evs n Bettepn opd6a anoréhece mv ondéa ehéyxou. Mecé mv Buata taw nexpayarsein Adri gOnoav puxpot peyéBoug rororendxra amd to puoxdipbi0 mpoc eneSepyaoria Kai napattipnon ovo mhextpovixs uxpooxdmo Srepxduevne Béoyine Kade Kat m rexviKt tne avoooioToxMHE!ag mpO¢ Mapattipnon FO ONUKE jt poox6mo pe e16ik6 povoxhovikd avnodpara évavn tou urofoxéa tav avépoyduav ae apatoam 1:20. Ennavaxés addoxdoers mapampsOnxav oe unepyuxpooKomns emine6o oro jtuordipbio. EnytensOnxe Srarapaxtt ‘orn Boyt wav capxopeplo Kat antoBiopydveon tng ypayyiie Z, Katd wéroue napovota o1SnparwSév Kat avknsé- vou peyéBouc nitoxovBplov Kai Kat prtOxdrEpIa Ge OUvINEN evs onHavUK fav Kat n UTapEn KOKKI@ YAUKO- yvou wera8i tov oapxopepiav. H avoooioroxnpuxh pehémn éBe1f mv mapovata tov avfpoyov1xos unoSoxéa oto xvttapénnaopa tav paptipav. Metd and tn xopriynon teotootepéene napamprénxe xard ténoue avényévn rcaon me avogoiotoxnyukg Xpdione 070 GapKéMAagHa Tw LvoKapDIaKGV KutEdpaW yeyoWK TOV avaxaromTpr- zr mnv ave éxppaon rou avSpoyovxo6 unoBoxéa. xoptiynon peydow Sdaewv woroorepéune otouc enfsves poxahel naBohoyink HoppohoVINE ahoxsoeIE O10 _Hvoxdp61o nou mBavic oxetizovtai pe mnv mapampotizem avknpévn éppaan rou av6poyovrKot uroboxéa. Ta evptipara curd unoompizouv ta anorerécprara didew pehersv nou ovaxetizouv mv efwyevti xopriynon Teotoote- pine pe uneprpowia rou puoKapBfou jécw tou avBpoyovIKos umoBoxéa. Aé&ere xde1616: teotootepdim, poppohoyiKs j1ehémn, avooojotoxnpK pehémn, pwoKdpé10, neypayatdzi0a TIATPIKR® 2006, 4: 24-28 Eroayaayh H Bioobveon ing teotootepdvng fexIv’ a6 tn x0- ‘dnovepéAn xa hapBaver xdpa ora éow yewnuxé xbeta- pa appévov Kar Onhéwv atdpav (xiittapa tou Leydig Kai @oOriKeE avtfotorxa) Kai otn SuxtUHTH zdvn TOU Gho108 tov emveppiBiav oe piKpétepes Hooduntes, Ot upidrepes Sptioesc efvar n Srappgwon wv Sevrepoye- Gv XepaKmnpiouKav tov @UAOU OTOUg dppeves KaL ‘ad€non xa1 B1atipnon tng libido, 01 avaBoiKég Bpd- oeig énwg n Biaripnon xa aifnon me puikig pazag at tng oomxtig muxWéentag Kat doKOSVTaL j1€00 TOU aV- Spoyov1Kos untoBoxéa.! Meré mv avaxdduifm tng testo- otepdvng to 1935 TAfOoc evepyenxay 161m éxouv, 23 Epyaonipo Iowioyias-Eyppvotoyias kat Avpenoioytas, Tua larpucs ALO, 2 | sofiaTBike Volunte |= Issue |, December 2006‘anoBo8ei ot xprion tng peta€é autav Oepaneia tng v- noyovipéuntag Kat tov Biatapaxdv ing libido wake Kan ‘avuynpavaxég 16iémnte¢? O1 avaBodaxég 16i6mnte¢ tv xaBiotouv 1baviK6 @appiaKevuxé j1é0 adEnong tg pU- lice rézag Kar anéBoan¢ otoug évtova aoxospevous a- Ohntés, Tig weheuraies 640 Bexaerieg weytiho népo¢ tng épewvag éxe1 emxevrpo8e! nepioosrepo ong Suopeveic emBpaoerg ing tectootepdvng nou Kupiiag efva n appe vortonuxh Spon ou yuvaikes, n KapBiaKi ueptpo- ia pe ouvéneia tny avénévn emintaon wv xapBiay- yeraxsv oupBapdrov? Kaxd mv xpévia efayevi xopri- yon Kai n peyaiétepn enirrison uneprpopiag tou npo- coxdtn.! Zug pépec jac onpexsiverat evbiagépov yia véec bide ing teotootepSvng Stag avuomnBayxIKés 1616- meg, n enfSpaon ov aOnpopauKA ihdKa, ym youre Biaxée erBpdoerc (non genomic effects) xaBkig Xai pe- raBohée omy éxppaon Kar Karavopt’ tov avépoyoviKod unoBoxéa oto xéttapo. Evd wéxpi npéuvos n nenof8n- on 61 n efwyevic xopnyodylevn teotootepévn fitav ev véhet toEiKh yia 10 puoKapBi0? véec pehétec tefvouv va avuxposoouy auuiv tnv ywéon. Ooov apopd om peta Bods ing éxppaong tov avépoyoriKos unoBoxéa onpel- Gvetar onpavuxs etepoyéveia ora eupiata neipayan- xév peReuiv. H napovoa epyacia éxet oxoné mn biepet- vnon popgohoyixsv unepyiKpooxomxy adhoidoewv Tou tux6v npoxahel n teotootepévn ve oUvéuaaLs je inv avoooiotoxnpuxth evtémon tov avSpoyovIKO UItO- Soxéa etd tnv xopriynon teotootepévnc, YAIKA KAI MEGOAOL Xpnorponoui@nxav dppeves Revxot entipveg ing gu- ig Wistar xapiopévor oe 860 od6ec, Fra neipaaté- 2a tng npdtng opd5ag xopnysiOnkav evSopuixd 10mg Teotootepévnc ent 20 ouvexelg mépec eved n Sedtepn ondba anoréhece thy onda edéyxov, Metd tnv tehev- tala npépa xopriynong, éha ta merpanarézea Buord- ‘omnxay etd and vépxaon kar éyive Alin tepaxiev tov apBiaKod ués. Opropéva e€ aura poviponorti@nxay ne didAvpa yhoutapaasediing 3% emi 840 Gpeg Kai pe Siddupa terpokeiSiov tov oopfou 1% ent pia dpa. Zn ‘ouvéxeia eyBantiornxay oe BidAupa ofexk00 oupavuhi- ‘ov 1% ent 16 pes (in tissue xpdon). AKodovnoe agu- Sdwwon pe vbauKd SrarGpata aibvAnKtig ahKosANG av- EaySpevng muxvémntag Kai eykheiopde Taw berypatov ‘oe pniives epon, Aemtés topes twv MapaoKevaopawv, orepa and xpdan pe xutpK6 phUB6O Kara Reynolds, napampAOnkav oe nhextpovik6 pKpooKdmo 61epxd- phevng Séonc, Adda 1ototeWdxta Kap51aKos j1v6¢ ton1o- Geci@nxay oe povoriointiKé 1éAULa PoPHEANG 10% nou napépervay Via xpovixé Sidompa 24-48 dpec, Axoho6Gnoe n cuviGng texvuxt erieRepyacia tov 1010- tepaxlov ya OXNLAUOL top KaI jUKPooxéman oto OTUXé pIKpooKSmO xaBKiG Kal N TEXVIKH tg avOOO!- Towns |= Teiyos |. AckévBios 2008 otoxnpeiag. Katé tv ywaou! avoooiotoxnpikth texvi- ah 01 topes KahGpOnKaY pe MpwCOVEVEs TOVIKS j10vO- xhuvixd aveiompa avépoyoviKos unodoxéa oe apafwon 1:20. Metd thy éxnttvon tov topdv éyive envdaon pe Seurepoyevés avtioopa (bionylated antibody on- gogenus) 0 Beppioxpacta Sopatiou ya 15 Aenea. H euipdvion tng avoooiatoxnpuxtic avtiSpaone éyrve pe e- Pappoytt Siaksparog xpapoysvou Siap1voBevzi5ivng DAB. Meté to Sérthupta tov topGv pe anootaypévo ve- 6 éyive ehagpd aveixpion pe arpato€vAivn Harris, a- @uBawoH Lous pe aviosa GeIpA aKooAdv KapBotv- Aovdhn xa xGAuN tv topSV pe B4Gayo tov Kava- 84. Axohov@noe n mapatiipnon oto oMUKS p1KPOOKS- mo. ATIOTEAEEMATA, ZayavUKh Uneprpopfa tov puoKapBiaKEy KUTKipaY kar ehdttwon tov Staxuttapixay Siaompdtav oe oxéon pe tou pdptupes onwerdOnke xatd inv rapacipnon oro OMUK6 |IKPOOKémO [le m xpdan aipatofuAivne-nadt- vn (EnKévec 1, 2). H avoooiotoxnjixti pekém mg evtémone tov av- Spoyovixod unoboxéa éBe1e mv snapEti tov ota guoio- doyiKd puoKapSiaKd Kittapa (Bem xpdon otOUG j1dp- Tupec Fixéva 3). Meta tm xopitynon teotootepévng na- patnpnhOnxe évtovn Geuxétnta tc avoooiatoxnpiKiie xpdons oto xutrapénihaopa onpavaKos apiSpod jivo- xapbiaxév xurtdpov (Enkéva 4). Haugen xpdon ep- nvedera o¢ abEnon tng éxcppaan¢ tou unoBoxéa. Katé my mapatiipnon oto nikextpovix6 p1KpooKS- mo anpeiOnkay onpavaxés noppohoyixés akoxsoe1¢ ‘o¢ oxéon pe ta puoxapBiaKa xoctapa in opdiac edgy” xou (Eva 5). 1 aihoxdoexc agopodoay Katd KipIo Ré6yo ta jt0- xév6p1a. AbEnon tov peyéBouc Kai tov apiOLod tv jutoxovdpiev Kau obvingn opiopévav e€ autdv (Exxva. 6) KaBa¢ enfong Kai Hapouofa or6npataBév pItOxoV- Exxéva 1. Yneprpogia tov pvoKap6iou, Aisatokudivn-noo!- vn x25 iaTn|Exx6va 2, Muoxap610 pdptupa. Amarofvhivn ewoivn X25. Splav pe ehdtwaon wav axpohopisy ing eowrepiKtic BI roxovBpiakiic pepBpdvne (Erkéva 7) Atay onpavuxé ‘evpripata. Annayés orn poppodoyia onyersOnxav Kat ota Gap- xouspia. Eupavizouy Batapaxi otn Boyt xat tov m1p0- cavatohopé tv puoiviBiav Kar anoBropyitvaon tn ‘yooupsic Z (Enxéva 8). Mera&s tv capxoyepiov rapa- mpeftar ofSnya Kai evaré0eon KoxKiav yAUKoyévoU (Exxéva 9). SYZHTHEH 01 avaBodsKée kar avépoyovixés Bpdgeig te te- crootepévng aoxodviai péow tov avBpoyoviKos uno- Boxéa. Adhes evépyeies aoxoivtai péow jin yovi61a- xév 8p6pav (non genomic effects) Snag n aénon tou evSoxuttdprou aoBeotiou Kai n evepyonoinon Tov Kivaodv wv npateiviv A Kai S.4 Enpavukés 6- og efvar 01 avagopé¢ rou unootpizouv tnv ager in Via to KapBiayyetaxs adotnpa eniBpaon tne te- owoatepévnc, Mehétn Katd mv onoia xopnyiiOnKe oe dppevec pe ota8epit omOdyxn SiabeppuKd xapnhi Eix6va 3, Qetuxi avocoiotoxnjuxsh xpdon oro puoK6ps10 ov paprupa, X25. 26) seat ra AEN La ie Lilie, Eix6va 4. Adgnon me évtaang mg avoooiotoxnpiKtic xpdong ped mn xopiynn coootepe, X28 {6an teotootepévnc éBeike otauoTIKGe onNavUK ab- €non tou xpévou ep@avionc mtdang tov ST>1mm Ka- 14 m Boxtpacia xénwong> AXAn pedérn avagéper pefoon ing abnpapanxig mhdKag oe KaAMEpyELeG KUTLAPAV MpoEPXopEvov and tv aopui KoviKhav, Spdon nov aoKeita: péow tou avBpoyoviKod unobo- xéas H Bocohoyia tng teotoatepdvnc oe Shec tic pene rec oxeuKd pe tc emBpacerg tg monKiher. Znyavaxd elvan nag xapniit Booohoyfa oBfynoe de mpootacia tov KapBlayye1aKos®® evs peydihec 6éaexc e”xav toEi- xée em8pdceig drag upBaiver ctouc abAntéc mou AapBavouv peyddec Béaer¢ avépoyévav. Ze auuh tv opd6a tov arépav undpxovy onpiavuxéc eménu0h0- yiKée adhd Kar arpoSwvapuxés peétee nov Seixvouv inv av€npévn entitaon epppdypiatog tou wuoxaps{ou He @voIohoyIKG otepaviaia ayyeia’ Kai ty Kap5iaKA uneprpopia,®? n onoia eivar avefdpmntog beixtng xapSiayyeraxfi¢ voonpérntag Kar Ovnorpétntac, To mio avnouxnuxé otorxeio eivar Ju o1 weraBoréc om Aenoupyia tov pwoKapBiou Kai n KapbLaKKi UNEpTPO- ia napapévouv ané Eva gag xa1 apKerd xpévia pera Exxéva 5. Tyiipa puoxapiaxot Kurtdpou opdba ehéyxov ‘votohoyixs ameiKévion Taw GapKOHEpIOV Kal Lav JUTOXOV- Spi. X10.000 Volume | — Issue |, December 2004Exxéva 6. Biivrinfn prroxov6pfov (BéAn), a’non vou api®pos Tov HITOXOVBpiav. Thinta yuoKapSraKO5 KUTpOU TieIpaya- "axtic op66a¢ X8000 tm Biaxorn tng xopriynonc teotootepévne,"? Exnv ma- potoa pehémn xopnyi@nxav peydher Bboeig teotoote- pdvng Mpocopordzovrac KataotdaeL¢ apEpores pe avréc mov Mpoxahotvta Katé ty Katéxpnon avafo- Iuxév avSpoysvev otepoeibiov. Or napamprioeic jag ia ug tofiKés Bpdaerg oe ureppIKpooKOMKS enineBo cuppavoiy pe autée GAdov epeuvntav mou onperd- ‘Vouv napépoia eupiizata pe peydan Bosohoyia teato- orepévng.? EvBiagépov oorxeso efvai n naparnpospevn ad- Enon ine éppaonc tov avépoyoviKod unoBoxéa. Mehétec pe PCR odtiynoay ae avexpoudpeva ano- tehéopata avefaptiitou tng apxiKeg xopnyotpevng 86on¢.6" Ané thy én n nooouxs pehém tou mR- NA tov avSpoyovixot umtooxéa pte PCR Sev oup- Badizer Kar’ avdyen pe tv Spaotuxétnta in vivo. Eun Sixt pag pedétn onpendverar avfnpévn évtaon tng avoooiotoxnpixiig xpdong oto xuttapémhaoya npaveKod apiOyos puoKapBraxdv xuTTapoVv peLd tm xoptiynon teotootepévng, H av€npévn éxgpaon Endiva 8. AnoBiopyéveon mc ypappig Z, Statapaxti ctov mpocavaronious tov yuoiviBiav. Nerpapatics onda 20000. tov vnoSoxéa tov avépoysveav mOaviic oxetizerat HE UG HoppOrOVIKEs aAAOISGeLG THOU TapatnpHOn- Kav 0 UnepUIKpooKoniKé enfe6o KaGig éxel arO- SerxOel and didouc epeuvntés 6u n umeprpogia TOV HUOKAPSIAKGV KUTtépeV aoKefta! Low tov av- Spoyovixos vno6oxéa Kai du n xopiiynon tov avea- Yoviott tov avBpoyévav (Kunpotepévn) anoxhefet Ihv uneprpogixt anévinon tov wuoKapSiou ctv teotootepdvn.? Tepaitépw perérn tov axpiBouc Broxnyxos Spspou péow tov onofou aoKosvtai o1 xapSioayyeiaxég Spdveig tng teotootepévng efvai avayxaia. H hentopeptic yvaon inc wpéhaine 6o- oohoyiag ahd Kai tov Buopevov emBpdoeav oto pvoxapS1ax6 xéttapo efvar amapaftnin yia inv TAnpéotepn xatavénon tov KiviKGY emmudoeov inc efoyevoc xopnyospevng teotoatepsvng Ge a- @antés, o€ vyith dtopa tpitng nhiKia we Gepaneia v- noKardotaong adAd Kai Ge KapBIOAoyIKOUG aode- vets. Eudva 7. Orény.aus6n jtoxsv6pra (BEn) pe eddtusem ww axpoloopisv ing eowrepinic prtoxovEpiaicic peppévne. er pantie opééa X20000. Tomos |= Tedyos |, AekénBpios 2005 Euxéva 9. Evboxuntdpio ofBnpia, napovata KoKkfuw yauKoyS- vou oto aapxérihagya pwoKapBiaKos KuedpoU HespazauKtig ‘oydibac X8000.Morphological and immunohistochemical study of the rat myocardium after testosterone administration Papamitsou 7, Deementzopoulou-Theodoridou M, BarlagiannisD, Kotouz A, Kotanidow B, Manthos A, Abstract ‘The principal androgen in male and female is testosterone, which along with its metabolite dihydrotestosterone, ‘exerts anabolic and androgenic effects through the androgen receptor. Non genomic effects are also well docu- ‘mented. Exogenous anabolic androgenic steroids and their influence on the performance and function of the heart, muscle, liver and other tissues have gained great interest. However, the usefulness of exogenous steroids jn the myocardium has not been documented. The aim of this study is to demonstrate the ultrastructural alter- ations caused in the rat heart when testosterone was administered in rats, as well as to explore immunohisto- chemically any changes in the expression of the androgen receptor. Two groups of adult male Wistar rats were used. The first group received an everyday intramuscular dose of 12mg testosterone enanthate for 20 days, hile the second group received placebo via the same route and for the same period of time, All rats were anaesthetized and sacrificed after the 20th day and tissue samples from the myocardium were received and ‘processed for transmission electron microscopy and immunohistochemistry using specific monocionic antibodies against the androgen receptor. Significant ultrastructural alterations were observed in the myocytes. We report pathological appearance of the sarcomeres’ structure including disorganization of the Z line. Mitochondrial ‘oedema and enlargement of the mitochondria were abundantly present. Mitochondrial fusion was significant as was the existence of glycogen particles in between the sarcomeres. Immunohistochemistry showed the expres- sion of the androgen receptor on the control group. When testosterone was administered, the staining for an- ‘drogen receptor in the myocytes was more dense which was considered as an increase in the expression of the androgen receptor. Exogenous testosterone produces pathological alterations in the structure of myocytes in the rat heart. The androgen receptor increases its expression and may play a role in the observed ultrastructural changes, These findings are in agreement with studies that report the androgen receptor mediated cardiac hy ertrophy caused by steroid abuse. Key words: testosterone, morphological study, immunohistochemical study, myocardium, rat 41, Anoatohdxng M. Erowyefa quatohoyiag tou vpsinou, Tonos [, 1996, SBN 960-85421- 13. 2. Payne JR, Kotwinski, Montogomery HE. Car- tac effects of anabolic steroids, Heart 2004 90 (6) A73:475. 3. Appel HJ, Hele-Umpfenbach B., Feraudl M, Weicker H. Ultrastructural and morphometric investigations on the eect of training and ad- ministration of anabolic steroids on the myo- cardium of guinea pigs, Internat J of Sport medicine 1983 4 (4): 268-274. 4. Heinlin GA, Chang C. The roles of androgen Receptor and androgen binding proteins in snongenomic androgen actions Molecular En- Aoctnology 2002 16(10) 2181-2187 28 apmiaTpiicn 5. English KM, Staeds RP, Jones TH, Diver MJ, Ghanner KS. Low dose transdermal ‘testosterone therapy improves angina thesh- ‘din men with chronic stable angina. Cr- culation 2000 102:1906, 8, Hanke H, Lenz C, Hess B, Spindler KD, Wel emann W. Efet of testosterone on plaque evelopment and androgen receptor expres- sion in arterial vessel wall. Circulation 2001 103: 1982-1385, 7. Parsinnen M, Kujala U, Vatiinen E, et a. Increased premature morally of compet tive powerifters suspected to have used an- abolic agents. Int J Sports Med 2000; 21: 225-227. 8, Thum , Borla J. Testosterone, Cytochrome 450 and cardiac hypertrophy. The FASEB ‘Juma, 2002;16:1537-1548. 9, Marsh JD, Lehmann MH, Rebecce HR, Gwath- ey JK, Green GE, Sshiebinger Rd. Androgen ‘receptors media hypertophy in carga myo cys, Creulton 1998 ; 98: 256-261 10. Urnausen A. Albers T and Kindermann W. Are the cardiac effects of anabolic steroid abuse in strength athletes reversible? Heart 2004;90:496-501. 11. Feldcoren Bl, Andresson S. Anabolic-andro- genic steroid interaction with rat androgen re- ceptor in vivo and in vitro: A comperative study. (article in press) J Steroid Biochem- isty & Molecular Biology (avalabe on line at vww.sciencedirect.com), Voluwe | — |ssue J, December 2006