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  • SJ-MM-019 - EN - Measurement of Hyaluronic Acid PDF

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    19 views1 page

    SJ-MM-019 - EN - Measurement of Hyaluronic Acid PDF

    Uploaded by

    Sandra

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Measurement of Hyaluronic acid [SJ-MM-019]

    2010-06-24
    Supersedes prior issues

    Quality Assurance
    Test procedure

    1. Add sample to 10ml volumetric flask.


    2. Add water and acetonitrile to 10ml. Solution and sonicate for 1hr.
    3. Centrifuge (3,500rpm, 10min) & Syringe filter (0.45um)
    4. Analysis
    5. Analysis condition
    Apparatus Model Agilent 1200 HPLC
    Column ZORBAX Eclipse XDB-C18 150 × 4.6mm I.D. 5um
    ZORBAX Eclipse XDB-C18 12.5 × 4.6mm I.D. 5um
    Mobile phase 10mM NaH2PO4 : Acetonitrile = 60 : 40
    Detector UV 210 nm
    Column oven temp 35℃
    Flow rate 0.45ml/min
    Injection volume 5ul

    SUNJIN CHEMICAL CO.,LTD.

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