SGOT (ASAT)KIT
(Mod, IFCC method}
Forth deteminton of SGOT (ASAT) aiviyn serum,
(For inviro Dagosic Use On)
Summary
‘SGOT Isanenymefound many inhertmusse, neces,
‘alta muscle ad rey. nyt ese dns resus
Inthe ras of the oxzymoin ood. Elevatd ives are
found. in myocardial infarction, Carése opratons,
Hepatts, Comes, acute panccalts, acate renal
iseases mary muscle Gseases, Deceased eves may
bsfeundinPregrancy es ar andDabetchooacioi
Principle
‘SGOT (ASAT) calles the transl of anino goup
between L-Aspariate and a Ketegutarate to fom
Onioaceate and Glusmate. The Oraoaclate formed
reactwi NADH nts prsenos of Malas Dehydrogenase
to fern NAD.The vate ‘of oxdaon of NADH to NAD is
measured a8. docreate in absorbance whch
Proportional lo tie SGOT (ASAT) actvyth sale.
Procedure
Wavelength fer Hoe
Temperature srcistcrae
Lghtpa ton
‘SubetrateSartAssay:
Pipette inioa candy teste labeled as Test).
‘addition a ym,
Sequence asciwe [arc
Enzyrs Reagent abn | cami
Sample ozm [orn
Treats ale aay leper fr thu and ad
Star Reagent 12) o2m | 02m
Mix wol and read he ill absarance A & repeat the
bsobance eag fer very 1,2, 83 minds, Calcul
mean abeabance change per minute
‘Sample Star Assay
Pipa into clean dy teste labotedas Tes):
‘Adlon o m
Soquence wciwe | are
Working Reagent | 10m Tom
Lisp» ergot Oninnitn + Ova
Normal eerence values
‘Seu (males) upto a7ULaLa7C
tamales)
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