Operator

instructions
Continuously
Adjustable
Pipettes

Jencons (Scientific) Ltd

Cherrycourt Way Ind. Est., Stanbridge Rd,
Leighton Buzzard, LU7 4UA, ENGLAND

Phone: 01342 826836 Int. Phone: +44 1525 372010

Fax: 01342 826771 Int. Fax: +44 1525 851461

Email: uksales@jencons.co.uk Website: www.jencons.co.uk

Int. Email: export@jencons.co.uk Online sales: www.ecomcat.co.uk

Jencons Scientific Inc (USA)

800 Bursca Drive, Suite 801
Bridgeville, PA 15017

Phone: 412 257-8861 Fax: 412 257-8809

Toll Free: 800 846-9959

Email: info@jenconsusa.com Website: www.jenconsusa.com

ISO 9001:2000

Ref: 401104
 Description
Contents
INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .This page
DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
VOLUME RANGES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 Push button Calibration screw
SETTING THE VOLUME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
PIPETTING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
GUIDELINES AND PRECAUTIONS . . . . . . . . . . . . . . . . . . . . . . .6
Tip ejector button
CALIBRATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
(not present on
PERFORMANCE SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . .7 480-205)
STORAGE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Volume
adjustment knob
LIMITATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Stainless steel
micrometer

Volume
INTRODUCTION indicator
Plastic handle
Thank you for purchasing our ACCURETTE single channel pipettor:
1. ACCURETTE aspirates and dispenses volumes of liquid
precisely and safely.
2. Six models of ACCURETTE continuously adjustable pipettes
cover the entire volume range from 1µl to 5000µl.
3. With the self-locking volume indicator, each model is Stainless steel
completely adjustable over its volume range. ejector arm
(not present on
4. A volume indicator makes it easy to set volume and to 480-205)
eliminate calculation errors.
5. A tip ejector button simplifies the discarding of the tip and
makes the operation safe. Plastic
shaft
6. Each ACCURETTE is factory calibrated and carefully checked
gravimetrically.

Disposable tip

Figure 1 (480-202 shown)

2 Operator Instructions Operator Instructions 3

VOLUME RANGES PIPETTING
Cat. No. Ranges 1. Hold ACCURETTE in one hand. With the other hand, turn the
480-200 1-10µl volume adjustment knob slowly until the volume indicator
480-201 2-20µl shows the desired volume. (Figure 2)
480-202 20-100µl 2. Attach a new disposable tip to the pipette cone. Press
480-203 50-200µl sufficiently to make a positive airtight seal. This avoid leaks or
poor accuracy.
480-204 100-1000µl
PRECAUTIONS:
480-205 1000-5000µl 1. The surfaces of tips, especially interior surface, must be clear, smooth and
hydrophobic to avoid retention of liquid. Too much retention results in poor
accuracy and reproducibility.
SETTING THE VOLUME 2. The tip orifice must be the correct size.
The required volume of liquid is set using the volume indicator.
3. Pre-rinse the tip by aspirating the first volume of liquid and
The volume indicator is read from top to bottom. For 480-200
then dispensing it back or to waste.
and 480-201, black digits indicate microlitres and red digits
4. Press the plunger to the first stop (position “1” shown in
tenths of microlitres; for 480-202 and 480-203, the bottom digits
Figure 3). This part of the stroke is the volume displayed on the
indicate microlitres; for 480-204 and 480-205, the bottom digits
volume indicator.
indicate ten times of microlitres.
5. Holding ACCURETTE vertically, immerse the tip into the liquid
480-200 480-201 480-202 480-203 480-204 480-205
to a depth of 2 – 4 mm. For 480-205, the immersion depth
0 1 0 1 0 1
2 2 2 2 2 2 should be 6 – 8 mm. (Jencons recommends a wide range of
5 5 5 5 5 5
tips for use with the Accurette range of pipettors).
2.5µl 12.5µl 25µl 125µl 250µl 1250µl
6. Wait 2 – 3 seconds. Allow the plunger to return slowly and
smoothly to the top position (position “0”). DO NOT let it snap
The volume is set by turning the volume adjustment knob
up. (Figure 4)
slowly to reach the required setting.
WARNING: 7. Withdraw the tip from the liquid after the full volume of
ALWAYS adjust the volume within the designated volume range. Do not turn the vol- sample is drawn into the tip.
ume adjustment knob with excessive force. This protects from inaccuracy and damage.
8. To dispense sample, place the tip end against the inside wall of
the recipient vessel at an angle of 10° to 40°. Depress the
plunger slowly and smoothly to the first stop (position “1”).
Wait 1 – 3 seconds. Then press the plunger to the second stop
(bottom of stroke – position “2”), expelling the residual liquid
from the tip. (Figure 5)
9. Withdraw ACCURETTE from the vessel carefully. Allow the
plunger to return to the top position (position “0”).
10. Discard the tip into suitable vessel by depressing the tip
ejector button. (Figure 6)

4 Operator Instructions Operator Instructions 5

Guidelines and Precautions Plunger Screw at
top of body
1. Select the suitable pipette and adjust the volume within the Upper part of
Screw
designed volume range. adjustment
knob
2. Change the tip for a different liquid to prevent sample carryover.
3. When using new tips, pre-rinse the tips by pipetting the liquid
several times to eliminate the chances of measuring error.
4. Change the tip if there is residual liquid at the end of tip.
5. Press and release the plunger at consistent speed and rhythm,
slowly and smoothly. Figure 7 Figure 8
6. NEVER invert or lay the ACCURETTE down with liquid in the
tip, thus avoiding liquid entering the plastic pipette shaft and Performance Specifications (Table 1)
damage to the piston.
When used in accordance with the pipetting procedures in this
7. When using a new ACCURETTE or a pipette which has not User’s Guide, ACCURETTE will perform to the specifications in
been used for a long time, press and release the plunger Table1.
several times before pipetting.
Cat. No. Volume Range Normal Volume Accuracy Precision
8. Do not pipette liquids which have extreme temperatures µl µl (mean error)% (repeatability) %
(>70°C, or <4°C) 1 ±4 ≤2.5
480-200 1-10 5 ±2 ≤1.0
9. ACCURETTE should never be used without a tip. 10 ±1.5 ≤0.8
2 ±5 ≤1.5
480-201 2-20 10 ±2 ≤1.0
20 ±2 ≤0.8
Calibration
20 ±2 ≤1.0
ACCURETTE is factory calibrated and carefully checked 480-202 20-100 50 ±1.2 ≤0.5
100 ±1.2 ≤0.3
gravimetrically before shipment using distilled water, an analytical 50 ±2 ≤1.0
480-203 50-200 100 ±1.0 ≤0.7
balance (accuracy is 1/10,000 to 1/100,000) and a beaker. 200 ±1.0 ≤0.5
100 ±1.5 ≤0.8
480-204 100-1000 500 ±0.9 ≤0.4
1000 ±0.9 ≤0.28
Recalibration
1000 ±1.1 ≤0.4
1. Loosen the three screws at the upper part of the volume 480-205 1000-5000 2000 ±0.7 ≤0.3
5000 ±0.7 ≤0.2
adjustment knob. (Figure 7).
2. Where the actual pipetting volume is less than that shown on NOTES:
1. Accuracy is the closeness of a measured volume to the true volume as specified by
the volume indicator, turn the screw positioned on top of the the volume setting of the pipette. Also known as “mean error”.
body counterclockwise (Figure 8); if the actual pipetting 2. Precision is the scatter of findings measurements around the mean of a large number
of replicate measurements of the same volume. Also known as “standard deviation”.
volume is more than that shown on the volume indicator, turn
the screw clockwise (Figure 8). Lock this adjustment, tighten the
three screws.
Storage
After use, store ACCURETTE in a clean, safe place.
3. Pipette the distilled water into the beaker following the
operation procedures and measure with an analytical balance.
Repeat the measurement six times. The results should be Limitations
consistent with the specifications in Table 1. Otherwise, calibrate
ACCURETTE will produce accurate and precise measurements of
and test again until the results are consistent with the
liquids with a density, viscosity and vapour pressure similar to
specifications in Table 1.
water.
PRECAUTION:
Water temperature and ambient conditions must be at 21.5°C ± 1°C before and
throughout the calibration procedures.

6 Operator Instructions Operator Instructions 7