NATIONAL INSTITUTE OF TECHNOLOGY ROURKELA

END-SEMESTER EXAMINATION, 2015

SESSION: 2015 – 2016 (autumn)
MSc. 3rd Semester

Subject code: LS-503 Subject Name: Plant Biotechnology Dept. Code: LS

No. of pages: 2 Full Marks: 50 Duration: 3 Hours

Figures at the right hand margin indicate marks.

All parts of a question should be answered at one place. Schematic diagram should be scientific.
Q. No Marks

1. A botanist is investigating a population of plants whose petal color is controlled by a

10
single gene whose two alleles (B & B1) are codominant. She finds 170 plants that are
homozygous brown, 340 plants that are homozygous purple and 21 plants whose
petals are purple-brown. Is this population in HWE (don't forget to do the proper
statistical test)? Calculate "F" (inbreeding coefficient) and explain what is happening
in this population.
Or,
A scientist has studied the amount of polymorphism in the alleles controlling the
enzyme Lactate Dehydrogenase (LDH) in a species of minnow. From one population,
1000 individuals were sampled. The scientist found the following frequencies of
genotypes: AA = 0.080, Aa = 0.280; aa = 0.640. From these data calculate the allele
frequencies of the "A" and "a" alleles in this population. Use the appropriate statistical
test to help you decide whether or not this population was in Hardy-Weinberg
equilibrium.

Chi-square table
2. What does it mean by speciation? Describe different types of reproductive isolating
10
barriers. How speciation is achieved in a population? (3+2+5)
3. What are the molecular markers based on PCR and use of restriction enzymes.
10
Explain them in detail. [3+7]
4. A. Provide the schematic diagram of the effects of non-disjunction in meiosis. [3
B. Explain homolog, ortholog, paralog with example. [3]
6
Or,
What are the reproductive isolation mechanisms? Explain them in detail. [6]

5. What is the difference between allopolyploidy and autopolyploidy? Explain 5

allopolyploidy with suitable example. [2+3]
6. How ionizing radiation causes mutation. Explain. [4]
4
Or,
How alkylating agents causes mutation? Explain. [4]
7. Explain codon volatility. [3]
3
Or,
Write notes on extranuclear inheritance. [3]
8. Explain the karyotype: 46,XX,del (5)(p25) in international description. [2]
Or, 2
What are the most frequent numerical anomalies in newborns? [2]