Analytical Methods:

Reference:

Alen Y, Suarmin O, Suci LN, Rivai H. Analysis of fatty acids levels of freeze-dried termite queen
Macrotermes gilvus Hagen using gas chromatography-mass spectrometry. International Journal of
Indegenous Herbs and Drugs (J. Ind. Herbs. Drugs), 2017; 2(4): 1-4.

Fatty acids analysis

The oil, powder and fresh form in a portion, 5 g of the ground sample was extracted for 8 h with 20
mL of n-hexane. Then 0.8 g of the extracted oil was saponified for 1 h with 15 mL of KOH in methanol
at 90 oC, treated with 2 ml of 1.4 N HCl for another 1 h and allowed to cool to room temperature. To
the cooled solution, 15 mL of n-heptane and 200 mL of brine were added, mixed thoroughly and 5 μL
of the fatty acid methyl esters (FAMEs) solution was analyzed with the aid of a high-performance gas
chromatography system (Hawlet, USA) for the fatty acids content. The FAMEs extract was coanalyzed
with authentic FAME standards (Sigma Chemicals, USA) of known structures.