<> 1 2 3 4 5 6

A 0.376 0.381 1.094 0.857 0.623 0.549

B 0.388 0.387 1.218 0.91 0.662 0.553
C 0.497 0.817 0.467 0.565 0.563 0.683
D 0.505 0.546 0.462 0.564 0.56 0.667
E 0.505 0.619 0.508 0.637 0.53 0.635
F 0.547 0.636 0.543 0.642 0.532 0.623
G 0.541 0.692 0.545 0.727 0.498 0.741
H 0.534 0.669 0.536 0.618 0.475 0.575

[Prot] Media R. Patrón

0 0.382969227
0.05 0.4379223675
0.1 0.5509963702
0.15 0.6422040174
0.3 0.8831024856
0.5 1.1543361729
 7 8 9 10 11 12
0.461 0.507 0.539 0.435 0.529 0.038
0.416 0.501 0.555 0.451 0.532 0.038
0.465 0.647 0.496 0.65 0.464 0.578
0.462 0.653 0.591 0.726 0.537 0.559
0.446 0.62 0.472 0.646 0.038 0.038
0.432 0.617 0.493 0.631 0.044 0.038
0.418 0.576 0.503 0.643 0.039 0.038
0.36 0.479 0.569 0.635 0.041 0.038
 Leyenda:
Sensibles Resistentes
Línea SK
Línea EFM
R. Calibrado
<> 1 2 3 4 5 6
A 0.376 0.381 1.094 0.857 0.623 0.549
B 0.388 0.387 1.218 0.91 0.662 0.553
C 0.497 0.817 0.467 0.565 0.563 0.683
D 0.505 0.546 0.462 0.564 0.56 0.667
E 0.505 0.619 0.508 0.637 0.53 0.635
F 0.547 0.636 0.543 0.642 0.532 0.623
G 0.541 0.692 0.545 0.727 0.498 0.741
H 0.534 0.669 0.536 0.618 0.475 0.575

Recta patrón
[P] (mg/mL) Abs
Blanco 0.382 0 0.3829692
0.05 0.4379224
0.1 0.5509964
0.15 0.642204
0.3 0.8831025
0.5 1.1543362 SK Sensibles

1.4

1.2
R² ==0.9950034978
f(x) 1.5729494577x + 0.3868810395
1
SK Resistentes
0.8

0.6

0.4

0.2
EFM Sensibles
0
0 0.1 0.2 0.3 0.4 0.5 0.6

EFM Resistentes
 7 8 9 10 11 12
0.461 0.507 0.539 0.435 0.529 0.038
0.416 0.501 0.555 0.451 0.532 0.038
0.465 0.647 0.496 0.65 0.464 0.578
0.462 0.653 0.591 0.726 0.537 0.559
0.446 0.62 0.472 0.646 0.038 0.038
0.432 0.617 0.493 0.631 0.044 0.038
0.418 0.576 0.503 0.643 0.039 0.038
0.36 0.479 0.569 0.635 0.041 0.038

Abs media mg/mL mg/mL Real

Control 1/40 0.5039910713 0.07444279 2.97771178
2.4616260095
Control 1/20 0.546941496 0.10174931 2.03498628
H 1/40 0.4429277593 0.03562067 1.42482699
1.6253633292
H 1/20 0.5304978794 0.09129498 1.82589967
INK 1/40 0.5009840317 0.07253101 2.90124055
3.2370892459
INK 1/20 0.6678937041 0.1786469 3.57293794
H + I 1/40 0.4644932723 0.04933134 1.97325379
2.1157500359
H + I 1/20 0.5644997786 0.11291231 2.25824628
Control 1/40 0.5614979964 0.11100388 4.44015504 Exp. 405. N2
4.0327323758
Control 1/20 0.6749525909 0.18313471 3.66269427
H 1/40 0.4634975728 0.04869831 1.94793243
2.5527371291
H 1/20 0.6499930769 0.16726625 3.3453249
INK 1/40 0.5414203543 0.09823915 3.92956588
3.8719824916
INK 1/20 0.6869497798 0.19076215 3.81524292
H + I 1/40 0.4991673066 0.071376 2.8550399
2.5670451943
H + I 1/20 0.5684206189 0.11540506 2.3081012
Control 1/40 0.5255806313 0.08816875 3.52675011
3.2843363798
Control 1/20 0.6274424276 0.15292926 3.05858513
H 1/40 0.52520853 0.08793218 3.5172873
3.3610953738
H 1/20 0.6394951134 0.16059197 3.21183945
INK 1/40 0.5309990584 0.09161362 3.66454469
3.3585063809
INK 1/20 0.6289713825 0.15390132 3.07802635
H + I 1/40 0.4389441878 0.03308805 1.32352185
1.9742291988
H + I 1/20 0.6184981811 0.14724279 2.94485576
H 72h 1/40 0.4823857378 0.06070681 2.42827231
2.7869580524
H 72h 1/20 0.6384559499 0.15993131 3.1986261
Control 1/40 0.5374886045 0.09573947 3.8295786 Exp. 408. N1
3.780472437
Control 1/20 0.6804028219 0.1865998 3.73199595
H 1/40 0.540481267 0.09764211 3.9056842
3.7514894594
H 1/20 0.6702879978 0.18016911 3.60338226
INK 1/40 0.4863640612 0.0632361 2.52944399
2.9240889231
INK 1/20 0.6527442072 0.16901533 3.38030653
H + I 1/40 0.38791752 0.000647 0.025876
1.7593699164
H + I 1/20 0.5252656471 0.0879685 1.75936992
H 72h 1/40 0.5349831773 0.09414659 3.76586375
3.4743405236
H 72h 1/20 0.6389874803 0.16026924 3.20538471
 [µg/µl] prom nº geles vol total vol prot
Control 1/40 ### 5.00 300 48.75
H 1/40 ### 5.00 300 73.83
SK Sensibles
INK 1/40 ### 5.00 300 37.07
H + I 1/40 ### 5.00 300 56.72
Control 1/40 ### 5.00 300 29.76
H 1/40 ### 5.00 300 47.01
SK Resistentes
INK 1/40 ### 5.00 300 30.99
H + I 1/40 ### 5.00 300 46.75
Control 1/40 ### 5.00 300 36.54
H 1/40 ### 5.00 300 35.70
EFM Sensibles INK 1/40 ### 5.00 300 35.73
H + I 1/40 ### 5.00 300 60.78
H 72h 1/40 ### 5.00 300 43.06
Control 1/40 ### 5.00 300 31.74
H 1/40 ### 5.00 300 31.99
EFM Resistentes INK 1/40 ### 5.00 300 41.04
H + I 1/40 ### 5.00 300 68.21
H 72h 1/40 ### 5.00 300 34.54

* El 4X se compone de la solución
4X y de 7% de ß-Mercaptoetanol.
Ej. 69,75 µL de 4X + 5,25 µL de
ß-mercaptoetanol.
 Volumen x
vol H2O 4X*
pocillo
176.25 75.00 60 Minimo 15microG por pocillo
151.17 75.00 vol 20ul cantidad 20ug ug totales
187.93 75.00
168.28 75.00
195.24 75.00
177.99 75.00
194.01 75.00
178.25 75.00
188.46 75.00
189.30 75.00
189.27 75.00
164.22 75.00
181.94 75.00
193.26 75.00
193.01 75.00
183.96 75.00
156.79 75.00
190.46 75.00
120