ERYTHROPOIESIS

STAGE SIZE NUCLEUS CYTOPLASM DIVISION LOCATION

SHAPE N:C COLOR CHROMATIN NUCLEOLI COLOR CONTENTS
PRONORMOBLAST -12-20µm -Round to -5:1 – 8:1 -Purple red -Open and fine but granular -1-2; have a -Dark/deep -Golgi complex -Mitosis; two -Present only in
slightly oval contains few fine clumps bluish tint blue -Mitochondria daughter Bone marrow
parachromatin is sparse pronormoblast

BASOPHILIC -10-15µm -Round; -4:1 – 6:1 -Purple -Coarse and begins to -0-1; but -Deep blue -Golgi complex -Mitosis; two -Present only in
NORMOBLAST Centered condense stains disappear later, -Many daughter Bone marrow
-Deep-purple red and usually not mitochondria polychromatic
visible normoblast
POLYCHROMATIC -10-12µm -Round; -1:1 – 4:1 -Red purple -Coarse and condense -None -Bluish pink to -Perinuclear halo -Last stage that -Present only in
NORMOBLAST Centered to -CHECKERBOARD grey-blue is capable of Bone marrow
eccentric APPEARANCE mitosis

ORTHOCHROMIC -8-10µm -Round; -1:4 – 1:2 -Blue purple -completely condensed and -None -Pink to -Hemoglobin -Not capable of -Present only in
NORMOBLAST Centered to homogenous (PYKNOTIC) orange-pink production mitosis Bone marrow
eccentric, may with a hint of increased
be fragmented blue -Organelles are
or extruding degraded
RETICULOCYTE -8-10µm -Pink with a tint -Remnants of golgi -Not capable of -Residues in
NO NUCLEUS of blue and mitochondria mitosis Bone marrow
-Residual RNA then moves into
peripheral
blood for about
1 day before
reaching
maturity
ERYTHROCYTE -6-8µm NO NUCLEUS -Salmon-pink -No mitochondria -Not capable of
with CENTRAL mitosis
PALLOR AREA
of about 1/3 of
the diameter of
the RBC
 LEUKOPOIESIS
STAGE SIZE NUCLEUS CYTOPLASM
SHAPE N:C COLOR CHROMATIN NUCLEOLI COLOR CONTENTS
1. MYELOBLAST 14-20µm -Round 8:1 – 4:1 -Reddish purple -Delicate and disperse -2-3 -Pale to -varying amounts of granules
deep blue
2. PROMYELOCYTE 16-25µm -Round or oval, eccentric 2:1 -Purple -Relatively fine -2-3 -Evenly -Full of primary or azurophilic
becoming coarser basophilic/ granules
blue
3. NEUTROPHILIC MYELOCYTE 12-18µm -Round, oval, or flattened on one 3:1 – 3:2 -Dark purple -Coarser chromatin -none visible -Pinkish -Variable number of non-
side pattern blue specific granules
4. NEUTROPHILIC 14-16µm -Typical kidney-bean, peanut- 7:3 – 1:1 -Dark purple -Coarse, blue-black -absent -Pinkish -Pinkish to reddish-blue
METAMYELOCYTE shaped or slightly indented chromatin blue granules
5. NEUTROPHILIC BAND CELL 9-12µm -Band-shaped, sausage-shaped, 1:1 – 1:2 -Pinkish-blue -Coarse, blue-black -absent -Pinkish -Pinkish to blue granules
markedly indented, highly chromatin blue
clumped; Indentation is greater
than half of the width of round
nucleus
6. POLYMORPHONUCLEAR 9-12µm -2-5 lobes connected by a 1:3 – 1:5 -Dark purple -Heavily clumped -None -Light pink -Many small evenly
NEUTROPHILS threadlike filament; nuclear to bluish distributed pink to rose-violet
indentation is > ½ of its diameter granules
3. EOSINOPHILIC MYELOCYTE 12-18µm -Round, oval, or flattened on one 3:1 – 3:2 -Dark purple -Coarser chromatin -None visible -Pinkish -Numerous large, round
side pattern blue specific granules staining
orange-brown to orange-blue;
variable numbers of non
specific granules
4. EOSINOPHILIC 14-16µm -Typical kidney-bean, peanut- 7:3 – 1:1 -Dark purple -Coarse, blue-black -Absent
METAMYELOCYTE shaped or slightly indented chromatin
5. EOSINOPHILIC BAND CELL 9-12µm -Band-shaped/ sausage-shaped/ 1:1 – 1:2 -Pinkish-blue -Coarse, blue-black -Pinkish -Numerous orange-red
markedly indented/ highly chromatin blue granules
clumped; Indentation is >1/2 of
the width of round nucleus
6. EOSINOPHILS 9-12µm -2 lobes usually 1:3 – 1:5 -Dark purple -Heavily clumped -None -Pink-blue -Many large, round, uniform
reddish-orange granules
3. BASOPHILIC MYELOCYTE 12-18µm -Round, oval, or flattened on one 3:1 – 3:2 -Dark purple -Coarser chromatin -None visible -Pinkish -Few large specific granules
side pattern blue staining dark bluish-purple to
bluish-black
4. BASOPHILIC METAMYELOCYTE 14-16µm -Typical kidney-bean, peanut- 7:3 – 1:1 -Dark purple -Coarse, blue-black -Absent -Pinkish -Few large, dark-blue black
shaped or slightly indented chromatin blue granules
5. BASOPHILIC BAND CELL 9-12µm -Band-shaped/ sausage-shaped/ 1:1 – 1:2 -Dark purple -Coarse, blue-black -None -Pink blue -Few large dark bluish-purple
markedly indented/ highly chromatin to bluish-black granules
clumped; indentation is > ½ of the
width of round nucleus
6. BASOPHILS 9-12µm -2 lobes usually obscured by 1:3 – 1:5 -Dark purple -Heavily clumped -None -Pink-blue -Numerous, dark blue-black
granules granules

7. MAST CELLS 9-12µm -Round 1:1 -Dark purple -Heavily clumped -None -Dark-blue -Many dark blue granules