Principle : spores would germinate after first steaming and destroyed on the next, three

steaming would eliminate all spores and their vegetative forms.

Low Temperature Steam.

this method is employed for sterilizing materials (blankets, polyethene tubing, etc).
which would be damaged at higher temperatures.

Steam at Temperatures Above 100 ° C (Autoclaving).

autoclaves are made of strong metal jackets; strong enough to withstand high pressures
requires (ffigs 2.1A to C). the autoclave door is hermetically sealed. it has safety valve
set to blow off at a predetermines pressure. the principle is that water boils when its
vapor pressure is equal to the pressure of the surrounding atmosphere. if the pressure
is raised inside a closed vessel, the temperature at which water boils will rise above
100ºC. at 15 lbs pressure water boils at 120ºC.

following are the measures that must be taken care of during autoclaving :

1. the steam must be saturated.

2. there must bes complete discharge of air from the sterilizing chamber.

3. the autoclave must be loaded in such a way that all the materials to be sterilized can
be adequately penetrated by steam.

4. the duration of autoclaving would depend on the pressure inside and hence on the
steam temperature.

Main idea : the principle of steam is spores would germinate after first
steaming and destroyed on the next, three steaming would eliminate all spores and their
vegetative forms. The principle of autoclave is that water boils when its vapor pressure
is equal to the pressure of the surrounding atmosphere

Supportive ide : Low Temperature Steam. This method is employed for sterilizing
materials (blankets, polyethene tubing, etc). which would be damaged at higher
temperatures. The autoclaves are made of strong metal jackets; strong enough to
withstand high pressures requires.
Conclusion : The autoclaves are made of strong metal jackets; strong enough
to withstand high pressures requires. if the pressure is raised inside a closed vessel, the
temperature at which water boils will rise above 100ºC. at 15 lbs pressure water boils at
120ºC. the principle of steam is spores would germinate after first steaming and
destroyed on the next, three steaming would eliminate all spores and their vegetative
forms.