FIRA SYAFIQAH BINTI SAIFUL RAHIM (SIO160005)

Select a microbe for the sustainable management and utilisation of agroresidues.

Microbe selected - Agro-residue selected - Grape skin

pulp (Winery waste)
Aureobasidium pullulan
(fungus)

Technology - Solid-state fermentation

in which hot water extracts of the pulp
can be as a good substrate (Israilides
Value-added product - Pullulan, a polysaccharide et al., 1998) and can be recycled for
polymer consisting of maltotriose unit. Pullulan fermentation with Aureobasidium
produced from winery waste was of high molecular pullulan for pullulan production
weight (4.22 x 106) and is pure as what have been (Arapoglou et al, 2002).
illustrated by its gel elucidation profile, glucose
content and the number of residues in repeating
unit (Israilides et al., 1994) if compared to using
other materials including orange peel, apricot and
molasses (Le Duy & Boa, 1982). Pullulan has many
industrial applications in the food, pharmaceutical,
and other fields. In the food industry, it is mainly
used as a starch substitute in low calorie foods, and
also as a bulking, binding agent in tablet
manufacturing in the pharmaceutical industry.

REFERENCES:
Arapoglou,D.,Israilides, C.J., Bocari,M.,Scanlon, B., Smith,A. (2002). Recycling Of grape skin pulp wastes (GSPW)
for the production of pullulan. Proceedings of the International Conference "Protection and Restoration
of the Environment VI’, July 1-5, Skiathos, Greece, pp 469-476.

Israilides, C. (1994). Characterization of pullulans produced from agro-industrial wastes. Carbohydrate

Polymers, 25(3), 203-209. doi:10.1016/0144-8617(94)90205-4

Israilides, C.J., Smith, A., HarthillJ.E., Barnett, C., Bambalov, G., Scanlon, B. (1998). Pullulan content of the
ethanol precipitate from fermented agro-industrial waste. Applied Microbiology and Biotechnology, 49,
613-617

Le Duy, A. & Boa, J. (1982). Pullulan production from peat hydrolysate. Canadian Journal of Microbiology, 2,
143–146