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Vet Pathol 34:189-198 (1997) Encephalitozoon heilem in Budgerigars (Melopsittacus undulatus) S. S. Back, L. A. Stemoukr,! D. C. Bertucct, L. B, Rocers, aN E. S. DIDIER Diagnostic Laboratory Services (SSB) and Animal Health Center (LAS), College of Veterinary Medicine, Mississippi State University, Mississippi State, MS; and Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, LA (DCB, LBR, ESD) Abstract. Microsporidiasis with concurrent megahacteriosis in hulgerigar (Molopsittarus undulatus) chicks contributed to significant economic loss in a commercial pet bird aviary in Mississippi. Three budgerigar chicks, 1-2 weeks old, from the aviary were necropsied. Microscopic lesions in the chicks consisted of heavy infection of enterocytes with microsporidia (2/3; autolysis precluded critical evaluation of the intestine of chick No. 2), ‘multifocal hepatic necrosis and inflammation with intralesional microsporidia (1/3), spherical clusters of mi- ‘crosporidia in the hepatic sinusoids in the absence of inflammation (1/3), and gastric megabacteriosis (3/3). The ultrastructure of the microsporidian spores was consistent with an Encephalitozoon species. The polymerase chia reaction sand Souler blot atalysis were used w ideutify die microsporidian as Encephuliwozvo hellem, an organism that has only been identified in humans. Encephalitozoon hellem causes keratoconjunctivitis and respiratory infections in humane with acquired immunodeficiency syndrome. This report presente the first con- firmed case of microsporidiosis in budgerigars. The finding of E. hellem in pet birds may be important in

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